Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
Identifieur interne : 000701 ( Istex/Curation ); précédent : 000700; suivant : 000702Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
Auteurs : Lili Liu [République populaire de Chine] ; Yanchun Yan [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Wei Wu [République populaire de Chine] ; Lei Xu [République populaire de Chine]Source :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2016-08.
Abstract
Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066–2073. © 2016 SETAC
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DOI: 10.1002/etc.3362
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type="main">Environmental Toxicology and Chemistry</title><title level="j" type="alt">ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY</title><idno type="ISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><imprint><biblScope unit="vol">35</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="2066">2066</biblScope><biblScope unit="page" to="2073">2073</biblScope><biblScope unit="page-count">8</biblScope><date type="published" when="2016-08">2016-08</date></imprint><idno type="ISSN">0730-7268</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0730-7268</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066–2073. © 2016 SETAC</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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