Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
Identifieur interne : 000702 ( Istex/Corpus ); précédent : 000701; suivant : 000703Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium
Auteurs : Lili Liu ; Yanchun Yan ; Jian Wang ; Wei Wu ; Lei XuSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2016-08.
Abstract
Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066–2073. © 2016 SETAC
Url:
DOI: 10.1002/etc.3362
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ISTEX:26A25B813C67C5D10201FD831266E2AAAD1DFFCELe document en format XML
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However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an <hi rend="italic">mt:egfp</hi> transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. <hi rend="italic">Environ Toxicol Chem</hi> 2016;35:2066–2073. © 2016 SETAC</p></abstract><textClass><keywords xml:lang="en"><term xml:id="etc3362-kwd-0001">Transgenic zebrafish</term><term xml:id="etc3362-kwd-0002">Biomonitoring</term><term xml:id="etc3362-kwd-0003">Zinc</term><term xml:id="etc3362-kwd-0004">Cadmium</term><term xml:id="etc3362-kwd-0005">Water quality criteria</term></keywords><keywords rend="articleCategory"><term>Environmental Toxicology</term></keywords><keywords rend="tocHeading1"><term>Environmental Toxicology</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-TRX6S6PM-6/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" xml:lang="en" type="serialArticle" xml:id="etc3362"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1002/(ISSN)1552-8618</doi><issn type="print">0730-7268</issn><issn type="electronic">1552-8618</issn><idGroup><id type="product" value="ETC"></id></idGroup><titleGroup><title type="main" sort="ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY">Environmental Toxicology and Chemistry</title><title type="short">Environ Toxicol Chem</title></titleGroup></publicationMeta><publicationMeta level="part" position="80"><doi registered="yes">10.1002/etc.v35.8</doi><copyright ownership="publisher">© 2016 SETAC</copyright><numberingGroup><numbering type="journalVolume" number="35">35</numbering><numbering type="journalIssue">8</numbering></numberingGroup><coverDate startDate="2016-08">August 2016</coverDate></publicationMeta><publicationMeta level="unit" position="230" type="article" status="forIssue"><doi>10.1002/etc.3362</doi><idGroup><id type="unit" value="ETC3362"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="articleCategory">Environmental Toxicology</title><title type="tocHeading1">Environmental Toxicology</title></titleGroup><copyright ownership="publisher">© 2016 SETAC</copyright><eventGroup><event type="manuscriptReceived" date="2015-12-04"></event><event type="manuscriptRevised" date="2015-12-13"></event><event type="manuscriptAccepted" date="2016-01-04"></event><event type="xmlCreated" agent="Thomson Digital" date="2016-05-04"></event><event type="firstOnline" date="2016-05-10"></event><event type="publishedOnlineAccepted" date="2016-01-11"></event><event type="publishedOnlineEarlyUnpaginated" date="2016-05-10"></event><event type="publishedOnlineFinalForm" date="2016-07-21"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:5.0.6 mode:FullText" date="2017-02-09"></event></eventGroup><numberingGroup><numbering type="pageFirst">2066</numbering><numbering type="pageLast">2073</numbering></numberingGroup><correspondenceTo><lineatedText><line>Address correspondence to
<email>yanyanchun@caas.cn</email></line></lineatedText></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ETC.ETC3362.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Generation of <i>mt:egfp</i> transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium</title><title type="short">A biosensor for detecting heavy metals</title><title type="shortAuthors">L. Liu et al</title></titleGroup><creators><creator xml:id="etc3362-cr-0001" creatorRole="author" affiliationRef="#etc3362-aff-0001"><personName><givenNames>Lili</givenNames> <familyName>Liu</familyName></personName></creator><creator xml:id="etc3362-cr-0002" creatorRole="author" affiliationRef="#etc3362-aff-0001" corresponding="yes"><personName><givenNames>Yanchun</givenNames> <familyName>Yan</familyName></personName></creator><creator xml:id="etc3362-cr-0003" creatorRole="author" affiliationRef="#etc3362-aff-0001"><personName><givenNames>Jian</givenNames> <familyName>Wang</familyName></personName></creator><creator xml:id="etc3362-cr-0004" creatorRole="author" affiliationRef="#etc3362-aff-0001"><personName><givenNames>Wei</givenNames> <familyName>Wu</familyName></personName></creator><creator xml:id="etc3362-cr-0005" creatorRole="author" affiliationRef="#etc3362-aff-0001"><personName><givenNames>Lei</givenNames> <familyName>Xu</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="etc3362-aff-0001" countryCode="CN"><orgName>Graduate School of Chinese Academy of Agricultural Sciences</orgName><address><city>Beijing</city><country>China</country></address></affiliation></affiliationGroup><keywordGroup type="author" xml:lang="en"><keyword xml:id="etc3362-kwd-0001">Transgenic zebrafish</keyword><keyword xml:id="etc3362-kwd-0002">Biomonitoring</keyword><keyword xml:id="etc3362-kwd-0003">Zinc</keyword><keyword xml:id="etc3362-kwd-0004">Cadmium</keyword><keyword xml:id="etc3362-kwd-0005">Water quality criteria</keyword></keywordGroup><fundingInfo><fundingAgency>Hi‐Tech Research and Development Program of China</fundingAgency><fundingNumber>2008AA10Z402</fundingNumber></fundingInfo><fundingInfo><fundingAgency fundRefName="National Natural Science Foundation of China" funderDoi="10.13039/501100001809">National Natural Science Foundation of China</fundingAgency><fundingNumber>31170119</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Basic Research Fund of Chinese Academy of Agricultural Sciences</fundingAgency><fundingNumber>0042012003</fundingNumber><fundingNumber>0042011006</fundingNumber></fundingInfo><supportingInformation><p>This article includes online‐only Supplemental Data.</p><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:07307268:media:etc3362:etc3362-sup-0001-SuppData-S1"></mediaResource><caption>Supporting Information.</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><section xml:id="etc3362-sec-0001"><p>Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an <i>mt:egfp</i> transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. <i>Environ Toxicol Chem</i> 2016;35:2066–2073. © 2016 SETAC</p></section></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>A biosensor for detecting heavy metals</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium</title></titleInfo><name type="personal"><namePart type="given">Lili</namePart><namePart type="family">Liu</namePart><affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yanchun</namePart><namePart type="family">Yan</namePart><affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China</affiliation><affiliation>Address correspondence to</affiliation><affiliation>E-mail: yanyanchun@caas.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jian</namePart><namePart type="family">Wang</namePart><affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wei</namePart><namePart type="family">Wu</namePart><affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lei</namePart><namePart type="family">Xu</namePart><affiliation>Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2016-08</dateIssued><dateCreated encoding="w3cdtf">2016-05-04</dateCreated><dateCaptured encoding="w3cdtf">2015-12-04</dateCaptured><dateValid encoding="w3cdtf">2016-01-04</dateValid><copyrightDate encoding="w3cdtf">2016</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal‐sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1‐cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose‐ and time‐dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. 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