[Detection of RNA of SARS coronavirus in hospital sewage].
Identifieur interne : 002C16 ( PubMed/Curation ); précédent : 002C15; suivant : 002C17[Detection of RNA of SARS coronavirus in hospital sewage].
Auteurs : Xin-Wei Wang [République populaire de Chine] ; Jin-Song Li ; Ting-Kai Guo ; Bei Zhen ; Qing-Xin Kong ; Bang Yi ; Zhong Li ; Nong Song ; Min Jin ; Wen-Jun Xiao ; Xiu-Mei Zhu ; Chang-Qing Gu ; Jing Yin ; Wei Wei ; Wei Yao ; Chao Liu ; Jian-Feng Li ; Guo-Rong Ou ; Min-Nian Wang ; Tong-Yu Fang ; Gui-Jie Wang ; Yao-Hui Qiu ; Huai-Huan Wu ; Fu-Huan Chao ; Jun-Wen LiSource :
- Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral, Nucléocapside.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Eaux d'égout, Syndrome respiratoire aigu sévère.
- Humains, Hôpitaux, RT-PCR.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- analysis : Nucleocapsid.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome, Sewage.
- Hospitals, Humans, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.
PubMed: 15312586
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002C16
Links to Exploration step
pubmed:15312586Le document en format XML
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<term>Humans</term>
<term>Nucleocapsid (analysis)</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
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<term>Eaux d'égout (virologie)</term>
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<term>Hôpitaux</term>
<term>Nucléocapside (analyse)</term>
<term>RT-PCR</term>
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<front><div type="abstract" xml:lang="en">In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.</div>
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<Month>01</Month>
<Day>07</Day>
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<Month>11</Month>
<Day>15</Day>
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<ArticleTitle>[Detection of RNA of SARS coronavirus in hospital sewage].</ArticleTitle>
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<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">A novel electropositive filter was used to concentrate the SARS-CoV from the sewage of two hospitals administered SARS patients in Beijing, including twelve 2,500 ml sewage samples from the hospitals before disinfection, and ten 25,000 ml samples after disinfection; as well as cell culture, RT-PCR and sequencing of gene to detect and identify the viruses from sewage.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">There was no live SARS-CoV detected in the sewage in this study. The nucleic acid of SARS-CoV had been found in the 12 sewage samples before disinfection from both hospitals by semi-nested PCR. After disinfection, SARS-CoV RNA could only be detected from the samples from the 309th Hospital, and the others were negative.</AbstractText>
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