Serveur d'exploration SRAS

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[Detection of RNA of SARS coronavirus in hospital sewage].

Identifieur interne : 002C16 ( PubMed/Curation ); précédent : 002C15; suivant : 002C17

[Detection of RNA of SARS coronavirus in hospital sewage].

Auteurs : Xin-Wei Wang [République populaire de Chine] ; Jin-Song Li ; Ting-Kai Guo ; Bei Zhen ; Qing-Xin Kong ; Bang Yi ; Zhong Li ; Nong Song ; Min Jin ; Wen-Jun Xiao ; Xiu-Mei Zhu ; Chang-Qing Gu ; Jing Yin ; Wei Wei ; Wei Yao ; Chao Liu ; Jian-Feng Li ; Guo-Rong Ou ; Min-Nian Wang ; Tong-Yu Fang ; Gui-Jie Wang ; Yao-Hui Qiu ; Huai-Huan Wu ; Fu-Huan Chao ; Jun-Wen Li

Source :

RBID : pubmed:15312586

Descripteurs français

English descriptors

Abstract

In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.

PubMed: 15312586

Links toward previous steps (curation, corpus...)


Links to Exploration step

pubmed:15312586

Le document en format XML

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<name sortKey="Li, Jin Song" sort="Li, Jin Song" uniqKey="Li J" first="Jin-Song" last="Li">Jin-Song Li</name>
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<name sortKey="Zhen, Bei" sort="Zhen, Bei" uniqKey="Zhen B" first="Bei" last="Zhen">Bei Zhen</name>
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<name sortKey="Li, Zhong" sort="Li, Zhong" uniqKey="Li Z" first="Zhong" last="Li">Zhong Li</name>
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<name sortKey="Gu, Chang Qing" sort="Gu, Chang Qing" uniqKey="Gu C" first="Chang-Qing" last="Gu">Chang-Qing Gu</name>
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<name sortKey="Yin, Jing" sort="Yin, Jing" uniqKey="Yin J" first="Jing" last="Yin">Jing Yin</name>
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<name sortKey="Wu, Huai Huan" sort="Wu, Huai Huan" uniqKey="Wu H" first="Huai-Huan" last="Wu">Huai-Huan Wu</name>
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<name sortKey="Chao, Fu Huan" sort="Chao, Fu Huan" uniqKey="Chao F" first="Fu-Huan" last="Chao">Fu-Huan Chao</name>
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<name sortKey="Li, Jun Wen" sort="Li, Jun Wen" uniqKey="Li J" first="Jun-Wen" last="Li">Jun-Wen Li</name>
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<title level="j">Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</title>
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<term>Hospitals</term>
<term>Humans</term>
<term>Nucleocapsid (analysis)</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Sewage (virology)</term>
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<term>ARN viral (analyse)</term>
<term>Eaux d'égout (virologie)</term>
<term>Humains</term>
<term>Hôpitaux</term>
<term>Nucléocapside (analyse)</term>
<term>RT-PCR</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<term>RNA, Viral</term>
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<term>ARN viral</term>
<term>Nucléocapside</term>
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<keywords scheme="MESH" qualifier="analysis" xml:lang="en">
<term>Nucleocapsid</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>SARS Virus</term>
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<term>Syndrome respiratoire aigu sévère</term>
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<term>Severe Acute Respiratory Syndrome</term>
<term>Sewage</term>
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<term>Hospitals</term>
<term>Humans</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
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<front>
<div type="abstract" xml:lang="en">In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.</div>
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<DateCompleted>
<Year>2005</Year>
<Month>01</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised>
<Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
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<ArticleTitle>[Detection of RNA of SARS coronavirus in hospital sewage].</ArticleTitle>
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<AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">In order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">A novel electropositive filter was used to concentrate the SARS-CoV from the sewage of two hospitals administered SARS patients in Beijing, including twelve 2,500 ml sewage samples from the hospitals before disinfection, and ten 25,000 ml samples after disinfection; as well as cell culture, RT-PCR and sequencing of gene to detect and identify the viruses from sewage.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">There was no live SARS-CoV detected in the sewage in this study. The nucleic acid of SARS-CoV had been found in the 12 sewage samples before disinfection from both hospitals by semi-nested PCR. After disinfection, SARS-CoV RNA could only be detected from the samples from the 309th Hospital, and the others were negative.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">It provides evidence that there is no live SARS-Cov in the sewage from hospitals with SARS patients though SARS-CoV RNA can be detected.</AbstractText>
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HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i   -Sk "pubmed:15312586" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1 

Wicri

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