SARS-CoV infection was from at least two origins in the Taiwan area.
Identifieur interne : 002806 ( PubMed/Curation ); précédent : 002805; suivant : 002807SARS-CoV infection was from at least two origins in the Taiwan area.
Auteurs : Mu-Chin Shih [Taïwan] ; Konan Peck ; Wen-Ling Chan ; Yen-Ping Chu ; Jui-Chang Chen ; Chang-Hai Tsai ; Jan-Gowth ChangSource :
- Intervirology [ 0300-5526 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN complémentaire (), ADN complémentaire (isolement et purification), ARN viral (génétique), ARN viral (isolement et purification), ARN viral (métabolisme), Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Données de séquences moléculaires, Délétion de séquence, Femelle, Humains, Mutation ponctuelle, Mâle, Phylogénie, RT-PCR, Réaction de polymérisation en chaîne, Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Taïwan, Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Épidémiologie moléculaire.
- MESH :
- génétique : ARN viral, Virus du SRAS.
- isolement et purification : ADN complémentaire, ARN viral, Virus du SRAS.
- métabolisme : ARN viral.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- ADN complémentaire, Adulte, Adulte d'âge moyen, Analyse de séquence d'ADN, Données de séquences moléculaires, Délétion de séquence, Femelle, Humains, Mutation ponctuelle, Mâle, Phylogénie, RT-PCR, Réaction de polymérisation en chaîne, Taïwan, Épidémiologie moléculaire.
- Wicri :
- geographic : Taïwan.
English descriptors
- KwdEn :
- Adult, DNA, Complementary (chemistry), DNA, Complementary (isolation & purification), Female, Humans, Male, Middle Aged, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, Point Mutation, Polymerase Chain Reaction, RNA, Viral (genetics), RNA, Viral (isolation & purification), RNA, Viral (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Analysis, DNA, Sequence Deletion, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Taiwan.
- MESH :
- chemical , chemistry : DNA, Complementary.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : DNA, Complementary, RNA, Viral.
- chemical , metabolism : RNA, Viral.
- geographic : Taiwan.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Female, Humans, Male, Middle Aged, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, Point Mutation, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Deletion.
Abstract
Severe acute respiratory syndrome (SARS) is caused by a new coronavirus. Genomic sequence analysis will provide the molecular epidemiology and help to develop vaccines.
DOI: 10.1159/000081739
PubMed: 15812185
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pubmed:15812185Le document en format XML
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<author><name sortKey="Shih, Mu Chin" sort="Shih, Mu Chin" uniqKey="Shih M" first="Mu-Chin" last="Shih">Mu-Chin Shih</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Medicine, China Medical University Hospital, Taichung, Taiwan.</nlm:affiliation>
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<wicri:regionArea>Department of Molecular Medicine, China Medical University Hospital, Taichung</wicri:regionArea>
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<author><name sortKey="Peck, Konan" sort="Peck, Konan" uniqKey="Peck K" first="Konan" last="Peck">Konan Peck</name>
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<author><name sortKey="Chan, Wen Ling" sort="Chan, Wen Ling" uniqKey="Chan W" first="Wen-Ling" last="Chan">Wen-Ling Chan</name>
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<author><name sortKey="Chen, Jui Chang" sort="Chen, Jui Chang" uniqKey="Chen J" first="Jui-Chang" last="Chen">Jui-Chang Chen</name>
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<term>Humans</term>
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<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
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<term>Molecular Epidemiology</term>
<term>Molecular Sequence Data</term>
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<term>Polymerase Chain Reaction</term>
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<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Analyse de séquence d'ADN</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
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<term>Phylogénie</term>
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<term>Réaction de polymérisation en chaîne</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by a new coronavirus. Genomic sequence analysis will provide the molecular epidemiology and help to develop vaccines.</div>
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<Month>08</Month>
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<DateRevised><Year>2011</Year>
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<ArticleTitle>SARS-CoV infection was from at least two origins in the Taiwan area.</ArticleTitle>
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<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Severe acute respiratory syndrome (SARS) is caused by a new coronavirus. Genomic sequence analysis will provide the molecular epidemiology and help to develop vaccines.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">We developed a rapid method to amplify and sequence the whole SARS-CoV genome from clinical specimens. The technique employed one-step multiplex RT-PCR to amplify the whole SARS-CoV genome, and then nested PCR was performed to amplify a 2-kb region separately. The PCR products were sequenced.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">We sequenced the genomes of SARS-CoV from 3 clinical specimens obtained in Taiwan. The sequences were similar to those reported by other groups, except that 17 single nucleotide variations and two 2-nucleotide deletions, and a 1-nucleotide deletion were found. All the variations in the clinical specimens did not alter the amino acid sequence. Of these 17 sequenced variants, two loci (positions 26203 and 27812) were segregated together as a specific genotype - T:T or C:C. Phylogenetic analysis showed two major clusters of SARS patients in Taiwan.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">We developed a very economical and rapid method to sequence the whole genome of SARS-CoV, which can avoid cultural influence. From our results, SARS patients in Taiwan may be infected from two different origins.</AbstractText>
<CopyrightInformation>Copyright (c) 2005 S. Karger AG, Basel.</CopyrightInformation>
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