SrasV1, PubMed, Curation, bibRecord, 002305

[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].

Identifieur interne : 002305 ( PubMed/Curation ); précédent : 002304; suivant : 002306

[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].

Auteurs : Li-Wen Qiu [République populaire de Chine] ; Ya-Di Wang ; Zhi-Yong Liao ; Kun Wen ; Xiao-Yan Che

Source :

Nan fang yi ke da xue xue bao = Journal of Southern Medical University [ 1673-4254 ] ; 2006.

RBID : pubmed:16546729

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Blotting, Western, Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (immunology), Coronavirus OC43, Human (genetics), Coronavirus OC43, Human (immunology), Cross Reactions (immunology), Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology).
MESH :
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal, Nucleocapsid Proteins, Recombinant Proteins.
- genetics : Coronavirus 229E, Human, Coronavirus OC43, Human, SARS Virus.
- immunology : Coronavirus 229E, Human, Coronavirus OC43, Human, Cross Reactions, SARS Virus.
- Animals, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Mice, Mice, Inbred BALB C.

Abstract

To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.

PubMed: 16546729

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Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].</title>
<author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name>
<affiliation wicri:level="1"><nlm:affiliation>Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Ya Di" sort="Wang, Ya Di" uniqKey="Wang Y" first="Ya-Di" last="Wang">Ya-Di Wang</name>
</author>
<author><name sortKey="Liao, Zhi Yong" sort="Liao, Zhi Yong" uniqKey="Liao Z" first="Zhi-Yong" last="Liao">Zhi-Yong Liao</name>
</author>
<author><name sortKey="Wen, Kun" sort="Wen, Kun" uniqKey="Wen K" first="Kun" last="Wen">Kun Wen</name>
</author>
<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
</author>
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<publicationStmt><idno type="wicri:source">PubMed</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].</title>
<author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name>
<affiliation wicri:level="1"><nlm:affiliation>Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wang, Ya Di" sort="Wang, Ya Di" uniqKey="Wang Y" first="Ya-Di" last="Wang">Ya-Di Wang</name>
</author>
<author><name sortKey="Liao, Zhi Yong" sort="Liao, Zhi Yong" uniqKey="Liao Z" first="Zhi-Yong" last="Liao">Zhi-Yong Liao</name>
</author>
<author><name sortKey="Wen, Kun" sort="Wen, Kun" uniqKey="Wen K" first="Kun" last="Wen">Kun Wen</name>
</author>
<author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name>
</author>
</analytic>
<series><title level="j">Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title>
<idno type="ISSN">1673-4254</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Blotting, Western</term>
<term>Coronavirus 229E, Human (genetics)</term>
<term>Coronavirus 229E, Human (immunology)</term>
<term>Coronavirus OC43, Human (genetics)</term>
<term>Coronavirus OC43, Human (immunology)</term>
<term>Cross Reactions (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Female</term>
<term>Fluorescent Antibody Technique, Indirect</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Coronavirus humain 229E (génétique)</term>
<term>Coronavirus humain 229E (immunologie)</term>
<term>Coronavirus humain OC43 (génétique)</term>
<term>Coronavirus humain OC43 (immunologie)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Réactions croisées (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Technique d'immunofluorescence indirecte</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Nucleocapsid Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus 229E, Human</term>
<term>Coronavirus OC43, Human</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus humain 229E</term>
<term>Coronavirus humain OC43</term>
<term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term>
<term>Coronavirus humain 229E</term>
<term>Coronavirus humain OC43</term>
<term>Protéines nucléocapside</term>
<term>Protéines recombinantes</term>
<term>Réactions croisées</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus 229E, Human</term>
<term>Coronavirus OC43, Human</term>
<term>Cross Reactions</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Female</term>
<term>Fluorescent Antibody Technique, Indirect</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Femelle</term>
<term>Humains</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Technique d'immunofluorescence indirecte</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16546729</PMID>
<DateCompleted><Year>2008</Year>
<Month>04</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>03</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1673-4254</ISSN>
<JournalIssue CitedMedium="Print"><Volume>26</Volume>
<Issue>3</Issue>
<PubDate><Year>2006</Year>
<Month>Mar</Month>
</PubDate>
</JournalIssue>
<Title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</Title>
<ISOAbbreviation>Nan Fang Yi Ke Da Xue Xue Bao</ISOAbbreviation>
</Journal>
<ArticleTitle>[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].</ArticleTitle>
<Pagination><MedlinePgn>290-3</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qiu</LastName>
<ForeName>Li-wen</ForeName>
<Initials>LW</Initials>
<AffiliationInfo><Affiliation>Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Ya-di</ForeName>
<Initials>YD</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liao</LastName>
<ForeName>Zhi-yong</ForeName>
<Initials>ZY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wen</LastName>
<ForeName>Kun</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Che</LastName>
<ForeName>Xiao-yan</ForeName>
<Initials>XY</Initials>
</Author>
</AuthorList>
<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Nan Fang Yi Ke Da Xue Xue Bao</MedlineTA>
<NlmUniqueID>101266132</NlmUniqueID>
<ISSNLinking>1673-4254</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D028941" MajorTopicYN="N">Coronavirus 229E, Human</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D028962" MajorTopicYN="N">Coronavirus OC43, Human</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003429" MajorTopicYN="N">Cross Reactions</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>3</Month>
<Day>21</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2008</Year>
<Month>4</Month>
<Day>15</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
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<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16546729</ArticleId>
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[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].

[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki