Optimization of a DNA vaccine against SARS.
Identifieur interne : 001D56 ( PubMed/Curation ); précédent : 001D55; suivant : 001D57Optimization of a DNA vaccine against SARS.
Auteurs : Alexander N. Zakhartchouk [Canada] ; Sathiyanarayanan Viswanathan ; Igor Moshynskyy ; Martin Petric ; Lorne A. BabiukSource :
- DNA and cell biology [ 1044-5498 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunité cellulaire, Plasmides, Production d'anticorps, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines virales structurales (génétique), Protéines virales structurales (immunologie), Signaux de triage des protéines, Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales structurales, Vaccins antiviraux, Vaccins à ADN.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales structurales, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS.
- Animaux, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Humains, Immunité cellulaire, Plasmides, Production d'anticorps, Signaux de triage des protéines, Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibody Formation, CD5 Antigens (genetics), Female, Humans, Immunity, Cellular, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred C57BL, Molecular Sequence Data, Plasmids, Protein Sorting Signals, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Structural Proteins (genetics), Viral Structural Proteins (immunology), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , genetics : CD5 Antigens, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins, Viral Structural Proteins, Viral Vaccines.
- chemical , immunology : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins, Viral Structural Proteins, Viral Vaccines.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Antibody Formation, Female, Humans, Immunity, Cellular, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Plasmids, Protein Sorting Signals, Spike Glycoprotein, Coronavirus.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) first appeared in Southern China in November 2002, and then quickly spread to 33 countries on five continents along international air travel routes. Although the SARS epidemic has been contained, there is a clear need for a safe and effective vaccine should an outbreak of a SARS-CoV infection reappear in human population. In this study, we tested four DNA-vaccine constructs: (1) pLL70, containing cDNA for the SARS-CoV spike (S) gene; (2) pcDNA-SS, containing codon-optimized S gene for SARS-CoV S protein (residues 12-1255) fused with a leader sequence derived from the human CD5 gene; (3) pcDNA-St, containing the gene encoding the N-portion of the codon-optimized S gene (residues 12-532) with the CD5 leader sequence; (4) pcDNA-St-VP22C, containing the gene encoding the N-portion of the codon-optimized S protein with the CD5 leader sequence fused with the C-terminal 138 amino acids of the bovine herpesvirus-1 (BHV-1) major tegument protein VP22. Each of these plasmids was intradermally administered to C57BL/6 mice in three separate immunizations. Analysis of humoral and cellular immune responses in immunized mice demonstrated that pcDNA-SS and pcDNA-St-VP22C are the most immunogenic SARS vaccine candidates.
DOI: 10.1089/dna.2007.0616
PubMed: 17665998
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pubmed:17665998Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) first appeared in Southern China in November 2002, and then quickly spread to 33 countries on five continents along international air travel routes. Although the SARS epidemic has been contained, there is a clear need for a safe and effective vaccine should an outbreak of a SARS-CoV infection reappear in human population. In this study, we tested four DNA-vaccine constructs: (1) pLL70, containing cDNA for the SARS-CoV spike (S) gene; (2) pcDNA-SS, containing codon-optimized S gene for SARS-CoV S protein (residues 12-1255) fused with a leader sequence derived from the human CD5 gene; (3) pcDNA-St, containing the gene encoding the N-portion of the codon-optimized S gene (residues 12-532) with the CD5 leader sequence; (4) pcDNA-St-VP22C, containing the gene encoding the N-portion of the codon-optimized S protein with the CD5 leader sequence fused with the C-terminal 138 amino acids of the bovine herpesvirus-1 (BHV-1) major tegument protein VP22. Each of these plasmids was intradermally administered to C57BL/6 mice in three separate immunizations. Analysis of humoral and cellular immune responses in immunized mice demonstrated that pcDNA-SS and pcDNA-St-VP22C are the most immunogenic SARS vaccine candidates.</div>
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<Abstract><AbstractText>Severe acute respiratory syndrome coronavirus (SARS-CoV) first appeared in Southern China in November 2002, and then quickly spread to 33 countries on five continents along international air travel routes. Although the SARS epidemic has been contained, there is a clear need for a safe and effective vaccine should an outbreak of a SARS-CoV infection reappear in human population. In this study, we tested four DNA-vaccine constructs: (1) pLL70, containing cDNA for the SARS-CoV spike (S) gene; (2) pcDNA-SS, containing codon-optimized S gene for SARS-CoV S protein (residues 12-1255) fused with a leader sequence derived from the human CD5 gene; (3) pcDNA-St, containing the gene encoding the N-portion of the codon-optimized S gene (residues 12-532) with the CD5 leader sequence; (4) pcDNA-St-VP22C, containing the gene encoding the N-portion of the codon-optimized S protein with the CD5 leader sequence fused with the C-terminal 138 amino acids of the bovine herpesvirus-1 (BHV-1) major tegument protein VP22. Each of these plasmids was intradermally administered to C57BL/6 mice in three separate immunizations. Analysis of humoral and cellular immune responses in immunized mice demonstrated that pcDNA-SS and pcDNA-St-VP22C are the most immunogenic SARS vaccine candidates.</AbstractText>
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