The spike protein of infectious bronchitis virus is retained intracellularly by a tyrosine motif.
Identifieur interne : 001C49 ( PubMed/Curation ); précédent : 001C48; suivant : 001C50The spike protein of infectious bronchitis virus is retained intracellularly by a tyrosine motif.
Auteurs : Christine Winter [Allemagne] ; Christel Schwegmann-Wessels ; Ulrich Neumann ; Georg HerrlerSource :
- Journal of virology [ 1098-5514 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Cricetinae, Endocytose, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (métabolisme), Immunoprécipitation, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (métabolisme), Technique d'immunofluorescence, Tyrosine (métabolisme), Virus de la bronchite infectieuse (métabolisme).
- MESH :
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Tyrosine, Virus de la bronchite infectieuse.
- Animaux, Cellules cultivées, Cricetinae, Endocytose, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Immunoprécipitation, Protéines de l'enveloppe virale, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Cricetinae, Endocytosis, Fluorescent Antibody Technique, Immunoprecipitation, Infectious bronchitis virus (metabolism), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (metabolism), Spike Glycoprotein, Coronavirus, Tyrosine (metabolism), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- metabolism : Infectious bronchitis virus, Membrane Glycoproteins, Tyrosine, Viral Envelope Proteins.
- Animals, Cells, Cultured, Cricetinae, Endocytosis, Fluorescent Antibody Technique, Immunoprecipitation, Spike Glycoprotein, Coronavirus.
Abstract
We have analyzed the intracellular transport of the spike (S) protein of infectious bronchitis virus (IBV), an avian coronavirus. Surface expression was analyzed by immunofluorescence microscopy, by surface biotinylation, and by syncytium formation by S-expressing cells. By applying these methods, the S protein was found to be retained intracellularly. Tyr1143 in the cytoplasmic tail was shown to be a crucial component of the retention signal. Deletion of a dilysine motif that has previously been suggested to function as a retrieval signal did not abolish intracellular retention. Treatment of the S proteins with endoglycosidases did not reveal any differences between the parental and the mutant proteins. Furthermore, all S proteins analyzed were posttranslationally cleaved into the subunits S1 and S2. In coexpression experiments, the S protein was found to colocalize with a Golgi marker. Taken together, these results indicate that the S protein of IBV is retained at a late Golgi compartment. Therefore, this viral surface protein differs from the S proteins of transmissible gastroenteritis virus and severe acute respiratory syndrome coronavirus, which are retained at a pre-Golgi compartment or transported to the cell surface, respectively. The implications of these differences are discussed.
DOI: 10.1128/JVI.02064-07
PubMed: 18094153
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<author><name sortKey="Winter, Christine" sort="Winter, Christine" uniqKey="Winter C" first="Christine" last="Winter">Christine Winter</name>
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<author><name sortKey="Schwegmann Wessels, Christel" sort="Schwegmann Wessels, Christel" uniqKey="Schwegmann Wessels C" first="Christel" last="Schwegmann-Wessels">Christel Schwegmann-Wessels</name>
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<author><name sortKey="Neumann, Ulrich" sort="Neumann, Ulrich" uniqKey="Neumann U" first="Ulrich" last="Neumann">Ulrich Neumann</name>
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<affiliation wicri:level="1"><nlm:affiliation>Institut für Virologie, Tierärztliche Hochschule Hannover, Bünteweg 17, 30559 Hannover, Germany. Christine.Winter@tiho-hannover.de</nlm:affiliation>
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<term>Infectious bronchitis virus (metabolism)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Tyrosine (metabolism)</term>
<term>Viral Envelope Proteins (chemistry)</term>
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<term>Glycoprotéines membranaires ()</term>
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<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Technique d'immunofluorescence</term>
<term>Tyrosine (métabolisme)</term>
<term>Virus de la bronchite infectieuse (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Infectious bronchitis virus</term>
<term>Membrane Glycoproteins</term>
<term>Tyrosine</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Tyrosine</term>
<term>Virus de la bronchite infectieuse</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cells, Cultured</term>
<term>Cricetinae</term>
<term>Endocytosis</term>
<term>Fluorescent Antibody Technique</term>
<term>Immunoprecipitation</term>
<term>Spike Glycoprotein, Coronavirus</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules cultivées</term>
<term>Cricetinae</term>
<term>Endocytose</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Immunoprécipitation</term>
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<front><div type="abstract" xml:lang="en">We have analyzed the intracellular transport of the spike (S) protein of infectious bronchitis virus (IBV), an avian coronavirus. Surface expression was analyzed by immunofluorescence microscopy, by surface biotinylation, and by syncytium formation by S-expressing cells. By applying these methods, the S protein was found to be retained intracellularly. Tyr1143 in the cytoplasmic tail was shown to be a crucial component of the retention signal. Deletion of a dilysine motif that has previously been suggested to function as a retrieval signal did not abolish intracellular retention. Treatment of the S proteins with endoglycosidases did not reveal any differences between the parental and the mutant proteins. Furthermore, all S proteins analyzed were posttranslationally cleaved into the subunits S1 and S2. In coexpression experiments, the S protein was found to colocalize with a Golgi marker. Taken together, these results indicate that the S protein of IBV is retained at a late Golgi compartment. Therefore, this viral surface protein differs from the S proteins of transmissible gastroenteritis virus and severe acute respiratory syndrome coronavirus, which are retained at a pre-Golgi compartment or transported to the cell surface, respectively. The implications of these differences are discussed.</div>
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<Abstract><AbstractText>We have analyzed the intracellular transport of the spike (S) protein of infectious bronchitis virus (IBV), an avian coronavirus. Surface expression was analyzed by immunofluorescence microscopy, by surface biotinylation, and by syncytium formation by S-expressing cells. By applying these methods, the S protein was found to be retained intracellularly. Tyr1143 in the cytoplasmic tail was shown to be a crucial component of the retention signal. Deletion of a dilysine motif that has previously been suggested to function as a retrieval signal did not abolish intracellular retention. Treatment of the S proteins with endoglycosidases did not reveal any differences between the parental and the mutant proteins. Furthermore, all S proteins analyzed were posttranslationally cleaved into the subunits S1 and S2. In coexpression experiments, the S protein was found to colocalize with a Golgi marker. Taken together, these results indicate that the S protein of IBV is retained at a late Golgi compartment. Therefore, this viral surface protein differs from the S proteins of transmissible gastroenteritis virus and severe acute respiratory syndrome coronavirus, which are retained at a pre-Golgi compartment or transported to the cell surface, respectively. The implications of these differences are discussed.</AbstractText>
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<ReferenceList><Reference><Citation>J Biol Chem. 2001 May 25;276(21):17887-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11359789</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2007 Mar;81(5):2418-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17166901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2001 Aug 24;276(34):31642-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11418598</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jun;78(11):5913-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15140989</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Oct 15;279(42):43661-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15304515</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Cell Biol. 1984 Mar;33(2):281-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6325194</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virus Res. 1986 Feb;4(2):133-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3010595</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 1986 Dec;67 ( Pt 12):2825-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3025348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1994 Oct;68(10):6523-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8083990</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1995 May 15;14(10):2257-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7774584</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 1996;141(6):1123-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8712929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Exp Med Biol. 1998;440:341-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9782301</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 1999 May 31;145(5):923-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10352010</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1999 Jul 1;163(1):25-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10384095</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Nov;79(21):13209-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16227244</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2006 May;87(Pt 5):1209-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16603523</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2001 May 18;276(20):17515-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11278557</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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