Serveur d'exploration SRAS

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Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.

Identifieur interne : 001704 ( PubMed/Curation ); précédent : 001703; suivant : 001705

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.

Auteurs : Hyun Kyoung Lee [Corée du Sud] ; Byoung Hee Lee ; Seung Hyeok Seok ; Min Won Baek ; Hui Young Lee ; Dong Jae Kim ; Yi Rang Na ; Kyoung Jin Noh ; Sung Hoon Park ; Dutta Noton Kumar ; Hiroaki Kariwa ; Mina Nakauchi ; Suk Jin Heo ; Jae Hak Park

Source :

RBID : pubmed:20458159

Descripteurs français

English descriptors

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

DOI: 10.4142/jvs.2010.11.2.165
PubMed: 20458159

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pubmed:20458159

Le document en format XML

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<term>Cross Reactions</term>
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<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
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<term>SARS Virus (immunology)</term>
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<term>Coronavirus humain OC43 (immunologie)</term>
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<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Réactions croisées</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
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<div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.</div>
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