The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production.
Identifieur interne : 001156 ( PubMed/Curation ); précédent : 001155; suivant : 001157The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production.
Auteurs : Ronghua Zhang [République populaire de Chine] ; Kai Wang [République populaire de Chine] ; Wei Lv [République populaire de Chine] ; Wenjing Yu [République populaire de Chine] ; Shiqi Xie [République populaire de Chine] ; Ke Xu [République populaire de Chine] ; Wolfgang Schwarz [République populaire de Chine] ; Sidong Xiong [République populaire de Chine] ; Bing Sun [République populaire de Chine]Source :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Viral Matrix Proteins.
- chemistry : Coronavirus 229E, Human.
- physiology : Coronavirus 229E, Human, Viral Matrix Proteins.
- Amino Acid Sequence, HEK293 Cells, Humans, Molecular Sequence Data, Protein Multimerization.
Abstract
In addition to a set of canonical genes, coronaviruses encode additional accessory proteins. A locus located between the spike and envelope genes is conserved in all coronaviruses and contains a complete or truncated open reading frame (ORF). Previously, we demonstrated that this locus, which contains the gene for accessory protein 3a from severe acute respiratory syndrome coronavirus (SARS-CoV), encodes a protein that forms ion channels and regulates virus release. In the current study, we explored whether the ORF4a protein of HCoV-229E has similar functions. Our findings revealed that the ORF4a proteins were expressed in infected cells and localized at the endoplasmic reticulum/Golgi intermediate compartment (ERGIC). The ORF4a proteins formed homo-oligomers through disulfide bridges and possessed ion channel activity in both Xenopus oocytes and yeast. Based on the measurement of conductance to different monovalent cations, the ORF4a was suggested to form a non-selective channel for monovalent cations, although Li(+) partially reduced the inward current. Furthermore, viral production decreased when the ORF4a protein expression was suppressed by siRNA in infected cells. Collectively, this evidence indicates that the HCoV-229E ORF4a protein is functionally analogous to the SARS-CoV 3a protein, which also acts as a viroporin that regulates virus production. This article is part of a Special Issue entitled: Viral Membrane Proteins - Channels for Cellular Networking.
DOI: 10.1016/j.bbamem.2013.07.025
PubMed: 23906728
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Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China; Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Kai" sort="Wang, Kai" uniqKey="Wang K" first="Kai" last="Wang">Kai Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Lv, Wei" sort="Lv, Wei" uniqKey="Lv W" first="Wei" last="Lv">Wei Lv</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Wenjing" sort="Yu, Wenjing" uniqKey="Yu W" first="Wenjing" last="Yu">Wenjing Yu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Shiqi" sort="Xie, Shiqi" uniqKey="Xie S" first="Shiqi" last="Xie">Shiqi Xie</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025</wicri:regionArea></affiliation></author><author><name sortKey="Schwarz, Wolfgang" sort="Schwarz, Wolfgang" uniqKey="Schwarz W" first="Wolfgang" last="Schwarz">Wolfgang Schwarz</name><affiliation wicri:level="1"><nlm:affiliation>Goethe-University Frankfurt, Institute for Biophysics, Max-von-Laue-Str. 1, D-60438 Frankfurt am Main, Germany; Shanghai Research Center for Acupuncture and Meridian, 199 Guoshoujing Road, Shanghai 201023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Goethe-University Frankfurt, Institute for Biophysics, Max-von-Laue-Str. 1, D-60438 Frankfurt am Main, Germany; Shanghai Research Center for Acupuncture and Meridian, 199 Guoshoujing Road, Shanghai 201023</wicri:regionArea></affiliation></author><author><name sortKey="Xiong, Sidong" sort="Xiong, Sidong" uniqKey="Xiong S" first="Sidong" last="Xiong">Sidong Xiong</name><affiliation wicri:level="1"><nlm:affiliation>Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China. Electronic address: sdxiongfd@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China; State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: bsun@sibs.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China; State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031</wicri:regionArea></affiliation></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Coronavirus 229E, Human (chemistry)</term><term>Coronavirus 229E, Human (physiology)</term><term>HEK293 Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Protein Multimerization</term><term>Viral Matrix Proteins (chemistry)</term><term>Viral Matrix Proteins (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules HEK293</term><term>Coronavirus humain 229E ()</term><term>Coronavirus humain 229E (physiologie)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Multimérisation de protéines</term><term>Protéines de la matrice virale ()</term><term>Protéines de la matrice virale (physiologie)</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Coronavirus 229E, Human</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus humain 229E</term><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus 229E, Human</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>HEK293 Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Protein Multimerization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules HEK293</term><term>Coronavirus humain 229E</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Multimérisation de protéines</term><term>Protéines de la matrice virale</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In addition to a set of canonical genes, coronaviruses encode additional accessory proteins. A locus located between the spike and envelope genes is conserved in all coronaviruses and contains a complete or truncated open reading frame (ORF). Previously, we demonstrated that this locus, which contains the gene for accessory protein 3a from severe acute respiratory syndrome coronavirus (SARS-CoV), encodes a protein that forms ion channels and regulates virus release. In the current study, we explored whether the ORF4a protein of HCoV-229E has similar functions. Our findings revealed that the ORF4a proteins were expressed in infected cells and localized at the endoplasmic reticulum/Golgi intermediate compartment (ERGIC). The ORF4a proteins formed homo-oligomers through disulfide bridges and possessed ion channel activity in both Xenopus oocytes and yeast. Based on the measurement of conductance to different monovalent cations, the ORF4a was suggested to form a non-selective channel for monovalent cations, although Li(+) partially reduced the inward current. Furthermore, viral production decreased when the ORF4a protein expression was suppressed by siRNA in infected cells. Collectively, this evidence indicates that the HCoV-229E ORF4a protein is functionally analogous to the SARS-CoV 3a protein, which also acts as a viroporin that regulates virus production. This article is part of a Special Issue entitled: Viral Membrane Proteins - Channels for Cellular Networking. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23906728</PMID><DateCompleted><Year>2014</Year><Month>04</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0006-3002</ISSN><JournalIssue CitedMedium="Print"><Volume>1838</Volume><Issue>4</Issue><PubDate><Year>2014</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Biochimica et biophysica acta</Title><ISOAbbreviation>Biochim. Biophys. Acta</ISOAbbreviation></Journal><ArticleTitle>The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production.</ArticleTitle><Pagination><MedlinePgn>1088-95</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbamem.2013.07.025</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0005-2736(13)00271-X</ELocationID><Abstract><AbstractText>In addition to a set of canonical genes, coronaviruses encode additional accessory proteins. A locus located between the spike and envelope genes is conserved in all coronaviruses and contains a complete or truncated open reading frame (ORF). Previously, we demonstrated that this locus, which contains the gene for accessory protein 3a from severe acute respiratory syndrome coronavirus (SARS-CoV), encodes a protein that forms ion channels and regulates virus release. In the current study, we explored whether the ORF4a protein of HCoV-229E has similar functions. Our findings revealed that the ORF4a proteins were expressed in infected cells and localized at the endoplasmic reticulum/Golgi intermediate compartment (ERGIC). The ORF4a proteins formed homo-oligomers through disulfide bridges and possessed ion channel activity in both Xenopus oocytes and yeast. Based on the measurement of conductance to different monovalent cations, the ORF4a was suggested to form a non-selective channel for monovalent cations, although Li(+) partially reduced the inward current. Furthermore, viral production decreased when the ORF4a protein expression was suppressed by siRNA in infected cells. Collectively, this evidence indicates that the HCoV-229E ORF4a protein is functionally analogous to the SARS-CoV 3a protein, which also acts as a viroporin that regulates virus production. This article is part of a Special Issue entitled: Viral Membrane Proteins - Channels for Cellular Networking. </AbstractText><CopyrightInformation>© 2013.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ronghua</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China; Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Kai</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Wenjing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Shiqi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ke</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schwarz</LastName><ForeName>Wolfgang</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Goethe-University Frankfurt, Institute for Biophysics, Max-von-Laue-Str. 1, D-60438 Frankfurt am Main, Germany; Shanghai Research Center for Acupuncture and Meridian, 199 Guoshoujing Road, Shanghai 201023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Sidong</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China. Electronic address: sdxiongfd@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Bing</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China; State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: bsun@sibs.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 AA020103</GrantID><Acronym>AA</Acronym><Agency>NIAAA NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>07</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biochim Biophys Acta</MedlineTA><NlmUniqueID>0217513</NlmUniqueID><ISSNLinking>0006-3002</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014763">Viral Matrix Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName 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IdType="pubmed">17658949</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 1997 Jan 10;272(2):1011-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8995396</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2009 Sep;5(9):e1000574</Citation><ArticleIdList><ArticleId IdType="pubmed">19730691</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2000 Jul 14;275(28):21203-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10747899</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochim Biophys Acta. 2002 Mar 19;1561(1):27-45</Citation><ArticleIdList><ArticleId IdType="pubmed">11988179</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2010 Sep 02;6(9):e1001087</Citation><ArticleIdList><ArticleId IdType="pubmed">20824094</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2011 Apr;90(1):64-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21356245</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochim Biophys Acta. 2011 Feb;1808(2):561-71</Citation><ArticleIdList><ArticleId IdType="pubmed">20546700</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Dec;79(23):14909-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16282490</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2012 Jun;86(12):6778-91</Citation><ArticleIdList><ArticleId IdType="pubmed">22496228</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Host Microbe. 2008 Apr 17;3(4):245-52</Citation><ArticleIdList><ArticleId IdType="pubmed">18342597</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Physiol. 2011 May;137(5):415-26</Citation><ArticleIdList><ArticleId IdType="pubmed">21518830</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Acta Pharmacol Sin. 2003 Oct;24(10):1051-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14531951</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochim Biophys Acta. 2011 Feb;1808(2):510-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20478263</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Rev Microbiol. 2012 Jul 02;10(8):563-74</Citation><ArticleIdList><ArticleId IdType="pubmed">22751485</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2010 Jan 15;7:6</Citation><ArticleIdList><ArticleId IdType="pubmed">20078868</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem J. 2009 May 13;420(2):295-303</Citation><ArticleIdList><ArticleId IdType="pubmed">19267691</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12540-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16894145</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2010 Mar 12;6(3):e1000801</Citation><ArticleIdList><ArticleId IdType="pubmed">20300659</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Vaccine. 2003 May 16;21(17-18):1833-42</Citation><ArticleIdList><ArticleId IdType="pubmed">12706667</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antimicrob Agents Chemother. 2010 Feb;54(2):835-45</Citation><ArticleIdList><ArticleId IdType="pubmed">19995924</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Adv Virus Res. 2006;66:193-292</Citation><ArticleIdList><ArticleId IdType="pubmed">16877062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2010 May;86(2):144-53</Citation><ArticleIdList><ArticleId IdType="pubmed">20156486</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Int Rev Cell Mol Biol. 2009;275:35-63</Citation><ArticleIdList><ArticleId IdType="pubmed">19491052</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2004 May 7;565(1-3):111-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15135062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2000 Mar 3;287(5458):1641-4</Citation><ArticleIdList><ArticleId IdType="pubmed">10698737</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proteins. 2008 Mar;70(4):1488-97</Citation><ArticleIdList><ArticleId IdType="pubmed">17910056</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Genes. 2008 Feb;36(1):95-104</Citation><ArticleIdList><ArticleId IdType="pubmed">17932736</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Cell Biol. 2001 May;3(5):473-83</Citation><ArticleIdList><ArticleId IdType="pubmed">11331875</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2003 Jan 30;535(1-3):34-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12560074</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 1992 May 1;69(3):517-28</Citation><ArticleIdList><ArticleId IdType="pubmed">1374685</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2009 Aug;83(16):7931-47</Citation><ArticleIdList><ArticleId IdType="pubmed">19515779</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1996 Oct;70(10):7108-15</Citation><ArticleIdList><ArticleId IdType="pubmed">8794357</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1967 Dec;58(6):2268-73</Citation><ArticleIdList><ArticleId IdType="pubmed">4298953</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Host Microbe. 2007 Sep 13;2(3):193-203</Citation><ArticleIdList><ArticleId IdType="pubmed">18005734</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2012 Nov 20;3(6):</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Int J Biochem Cell Biol. 2009 Nov;41(11):2232-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19398035</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2007 Nov;88(Pt 11):3067-77</Citation><ArticleIdList><ArticleId IdType="pubmed">17947532</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2004 Jul 30;341(1):271-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15312778</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):6723-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15194747</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Res. 2011 Aug;21(8):1271-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21747413</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Arch Virol. 2012 Jul;157(7):1411-22</Citation><ArticleIdList><ArticleId IdType="pubmed">22481600</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2004 Apr;10(4):368-73</Citation><ArticleIdList><ArticleId IdType="pubmed">15034574</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Jan;79(2):884-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15613317</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biophys J. 2010 Sep 22;99(6):1718-25</Citation><ArticleIdList><ArticleId IdType="pubmed">20858415</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2012 Feb 17;586(4):384-91</Citation><ArticleIdList><ArticleId IdType="pubmed">22245155</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 1992 May 15;69(4):577-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1375129</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 1991 Feb;180(2):617-24</Citation><ArticleIdList><ArticleId IdType="pubmed">1989386</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Membr Biol. 1983;76(3):197-225</Citation><ArticleIdList><ArticleId IdType="pubmed">6100862</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2010 Nov 30;1(5):</Citation><ArticleIdList><ArticleId IdType="pubmed">21151776</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Soc Exp Biol Med. 1966 Jan;121(1):190-3</Citation><ArticleIdList><ArticleId IdType="pubmed">4285768</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2003 Sep 18;552(1):28-34</Citation><ArticleIdList><ArticleId IdType="pubmed">12972148</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2004 Jan 16;557(1-3):99-103</Citation><ArticleIdList><ArticleId IdType="pubmed">14741348</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2011 Jun;7(6):e1002116</Citation><ArticleIdList><ArticleId IdType="pubmed">21738474</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell. 2004 Apr 23;14(2):259-67</Citation><ArticleIdList><ArticleId IdType="pubmed">15099524</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 May 13;100(10):6104-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12719519</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2005 May;109(2):191-202</Citation><ArticleIdList><ArticleId IdType="pubmed">15763150</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS Pathog. 2009 May;5(5):e1000450</Citation><ArticleIdList><ArticleId IdType="pubmed">19478868</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId 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