Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Identifieur interne : 000837 ( PubMed/Curation ); précédent : 000836; suivant : 000838Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Auteurs : Victor M. Corman [Allemagne] ; Olfert Landt [Allemagne] ; Marco Kaiser [Allemagne] ; Richard Molenkamp [Pays-Bas] ; Adam Meijer [Pays-Bas] ; Daniel Kw Chu [République populaire de Chine] ; Tobias Bleicker [Allemagne] ; Sebastian Brünink [Allemagne] ; Julia Schneider [Allemagne] ; Marie Luisa Schmidt [Allemagne] ; Daphne Gjc Mulders [Pays-Bas] ; Bart L. Haagmans [Pays-Bas] ; Bas Van Der Veer [Pays-Bas] ; Sharon Van Den Brink [Pays-Bas] ; Lisa Wijsman [Pays-Bas] ; Gabriel Goderski [Pays-Bas] ; Jean-Louis Romette [France] ; Joanna Ellis [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Malik Peiris [République populaire de Chine] ; Herman Goossens [Belgique] ; Chantal Reusken [Pays-Bas] ; Marion Pg Koopmans [Pays-Bas] ; Christian Drosten [Allemagne]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Flambées de maladies, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Techniques de laboratoire clinique ().
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections à coronavirus.
- Coronavirus, Flambées de maladies, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de laboratoire clinique.
English descriptors
- KwdEn :
- Clinical Laboratory Techniques (methods), Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Disease Outbreaks, Humans, RNA, Viral (analysis), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity.
- MESH :
- chemical , analysis : RNA, Viral.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Clinical Laboratory Techniques, Real-Time Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Disease Outbreaks, Humans, Sensitivity and Specificity.
Abstract
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.
DOI: 10.2807/1560-7917.ES.2020.25.3.2000045
PubMed: 31992387
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xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name><affiliation wicri:level="1"><nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" 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uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><affiliation wicri:level="1"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno 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Corman</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Landt, Olfert" sort="Landt, Olfert" uniqKey="Landt O" first="Olfert" last="Landt">Olfert Landt</name><affiliation wicri:level="1"><nlm:affiliation>Tib-Molbiol, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name><affiliation wicri:level="1"><nlm:affiliation>Tib-Molbiol, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Molenkamp, Richard" sort="Molenkamp, Richard" uniqKey="Molenkamp R" first="Richard" last="Molenkamp">Richard Molenkamp</name><affiliation wicri:level="1"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name><affiliation wicri:level="1"><nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author><author><name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name><affiliation wicri:level="1"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="1"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name><affiliation wicri:level="1"><nlm:affiliation>Universite d Aix-Marseille, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Universite d Aix-Marseille, Marseille</wicri:regionArea></affiliation></author><author><name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name><affiliation wicri:level="1"><nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name><affiliation wicri:level="1"><nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp</wicri:regionArea></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea></affiliation></author><author><name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><affiliation wicri:level="1"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea></affiliation></author></analytic><series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title><idno type="eISSN">1560-7917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clinical Laboratory Techniques (methods)</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Disease Outbreaks</term><term>Humans</term><term>RNA, Viral (analysis)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Flambées de maladies</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus</term><term>Flambées de maladies</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31992387</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1560-7917</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>01</Month></PubDate></JournalIssue><Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title><ISOAbbreviation>Euro Surveill.</ISOAbbreviation></Journal><ArticleTitle>Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.2807/1560-7917.ES.2020.25.3.2000045</ELocationID><Abstract><AbstractText Label="BACKGROUND">The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</AbstractText><AbstractText Label="AIM">We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.</AbstractText><AbstractText Label="METHODS">Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.</AbstractText><AbstractText Label="RESULTS">The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project.</AbstractText><AbstractText Label="CONCLUSION">The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Corman</LastName><ForeName>Victor M</ForeName><Initials>VM</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Landt</LastName><ForeName>Olfert</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Tib-Molbiol, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaiser</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Tib-Molbiol, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Molenkamp</LastName><ForeName>Richard</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meijer</LastName><ForeName>Adam</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chu</LastName><ForeName>Daniel Kw</ForeName><Initials>DK</Initials><AffiliationInfo><Affiliation>University of Hong Kong, Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bleicker</LastName><ForeName>Tobias</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brünink</LastName><ForeName>Sebastian</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schneider</LastName><ForeName>Julia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>Marie Luisa</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mulders</LastName><ForeName>Daphne Gjc</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haagmans</LastName><ForeName>Bart L</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van der Veer</LastName><ForeName>Bas</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van den Brink</LastName><ForeName>Sharon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wijsman</LastName><ForeName>Lisa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goderski</LastName><ForeName>Gabriel</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Romette</LastName><ForeName>Jean-Louis</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Universite d Aix-Marseille, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>Joanna</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Public Health England, London, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zambon</LastName><ForeName>Maria</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Public Health England, London, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peiris</LastName><ForeName>Malik</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University of Hong Kong, Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goossens</LastName><ForeName>Herman</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reusken</LastName><ForeName>Chantal</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koopmans</LastName><ForeName>Marion Pg</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Drosten</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Sweden</Country><MedlineTA>Euro Surveill</MedlineTA><NlmUniqueID>100887452</NlmUniqueID><ISSNLinking>1025-496X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Protocol" UI="C000657964">COVID-19 diagnostic testing</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Euro Surveill. 2020 Mar;25(9):</RefSource><PMID Version="1">32156329</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D019411" MajorTopicYN="Y">Clinical Laboratory Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">2019-nCoV</Keyword><Keyword MajorTopicYN="Y">RT-PCR</Keyword><Keyword MajorTopicYN="Y">Wuhan</Keyword><Keyword MajorTopicYN="Y">diagnostics</Keyword><Keyword MajorTopicYN="Y">laboratory</Keyword><Keyword MajorTopicYN="Y">novel coronavirus</Keyword><Keyword MajorTopicYN="Y">outbreak</Keyword><Keyword MajorTopicYN="Y">testing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId><ArticleId IdType="doi">10.2807/1560-7917.ES.2020.25.3.2000045</ArticleId><ArticleId IdType="pmc">PMC6988269</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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