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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.

Identifieur interne : 000837 ( PubMed/Corpus ); précédent : 000836; suivant : 000838

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.

Auteurs : Victor M. Corman ; Olfert Landt ; Marco Kaiser ; Richard Molenkamp ; Adam Meijer ; Daniel Kw Chu ; Tobias Bleicker ; Sebastian Brünink ; Julia Schneider ; Marie Luisa Schmidt ; Daphne Gjc Mulders ; Bart L. Haagmans ; Bas Van Der Veer ; Sharon Van Den Brink ; Lisa Wijsman ; Gabriel Goderski ; Jean-Louis Romette ; Joanna Ellis ; Maria Zambon ; Malik Peiris ; Herman Goossens ; Chantal Reusken ; Marion Pg Koopmans ; Christian Drosten

Source :

RBID : pubmed:31992387

English descriptors

Abstract

The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.

DOI: 10.2807/1560-7917.ES.2020.25.3.2000045
PubMed: 31992387

Links to Exploration step

pubmed:31992387

Le document en format XML

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<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
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<name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name>
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<nlm:affiliation>Tib-Molbiol, Berlin, Germany.</nlm:affiliation>
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<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
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<name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name>
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<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
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<name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name>
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<name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name>
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<name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name>
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<name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name>
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<name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name>
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<name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name>
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<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
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<name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name>
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<name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name>
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<name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name>
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<name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name>
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<name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name>
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<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
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<name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name>
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<nlm:affiliation>Universite d Aix-Marseille, Marseille, France.</nlm:affiliation>
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<name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name>
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<nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation>
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<name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
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<nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation>
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<name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name>
<affiliation>
<nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation>
</affiliation>
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<name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name>
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<nlm:affiliation>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium.</nlm:affiliation>
</affiliation>
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<name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
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<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
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<name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name>
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<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
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<name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
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<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
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<name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name>
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<nlm:affiliation>Tib-Molbiol, Berlin, Germany.</nlm:affiliation>
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<name sortKey="Molenkamp, Richard" sort="Molenkamp, Richard" uniqKey="Molenkamp R" first="Richard" last="Molenkamp">Richard Molenkamp</name>
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<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
</affiliation>
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<name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name>
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<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
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<author>
<name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name>
<affiliation>
<nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation>
</affiliation>
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<author>
<name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name>
<affiliation>
<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
</affiliation>
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<author>
<name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name>
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<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
</affiliation>
</author>
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<name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name>
<affiliation>
<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name>
<affiliation>
<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name>
<affiliation>
<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name>
<affiliation>
<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
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<author>
<name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name>
<affiliation>
<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name>
<affiliation>
<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name>
<affiliation>
<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name>
<affiliation>
<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name>
<affiliation>
<nlm:affiliation>Universite d Aix-Marseille, Marseille, France.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name>
<affiliation>
<nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
<affiliation>
<nlm:affiliation>Public Health England, London, United Kingdom.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name>
<affiliation>
<nlm:affiliation>University of Hong Kong, Hong Kong, China.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name>
<affiliation>
<nlm:affiliation>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
<affiliation>
<nlm:affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name>
<affiliation>
<nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
<affiliation>
<nlm:affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</nlm:affiliation>
</affiliation>
</author>
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<series>
<title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title>
<idno type="eISSN">1560-7917</idno>
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<date when="2020" type="published">2020</date>
</imprint>
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<term>Clinical Laboratory Techniques (methods)</term>
<term>Coronavirus (classification)</term>
<term>Coronavirus (genetics)</term>
<term>Coronavirus (isolation & purification)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>RNA, Viral (analysis)</term>
<term>Real-Time Polymerase Chain Reaction (methods)</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en">
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en">
<term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en">
<term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en">
<term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en">
<term>Clinical Laboratory Techniques</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en">
<term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>Sensitivity and Specificity</term>
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<front>
<div type="abstract" xml:lang="en">The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</div>
</front>
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<MedlineCitation Status="MEDLINE" Owner="NLM">
<PMID Version="1">31992387</PMID>
<DateCompleted>
<Year>2020</Year>
<Month>02</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised>
<Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print">
<Journal>
<ISSN IssnType="Electronic">1560-7917</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>25</Volume>
<Issue>3</Issue>
<PubDate>
<Year>2020</Year>
<Month>01</Month>
</PubDate>
</JournalIssue>
<Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title>
<ISOAbbreviation>Euro Surveill.</ISOAbbreviation>
</Journal>
<ArticleTitle>Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.</ArticleTitle>
<ELocationID EIdType="doi" ValidYN="Y">10.2807/1560-7917.ES.2020.25.3.2000045</ELocationID>
<Abstract>
<AbstractText Label="BACKGROUND">The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</AbstractText>
<AbstractText Label="AIM">We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.</AbstractText>
<AbstractText Label="METHODS">Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.</AbstractText>
<AbstractText Label="RESULTS">The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project.</AbstractText>
<AbstractText Label="CONCLUSION">The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Corman</LastName>
<ForeName>Victor M</ForeName>
<Initials>VM</Initials>
<AffiliationInfo>
<Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Landt</LastName>
<ForeName>Olfert</ForeName>
<Initials>O</Initials>
<AffiliationInfo>
<Affiliation>Tib-Molbiol, Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Kaiser</LastName>
<ForeName>Marco</ForeName>
<Initials>M</Initials>
<AffiliationInfo>
<Affiliation>Tib-Molbiol, Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Molenkamp</LastName>
<ForeName>Richard</ForeName>
<Initials>R</Initials>
<AffiliationInfo>
<Affiliation>Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Meijer</LastName>
<ForeName>Adam</ForeName>
<Initials>A</Initials>
<AffiliationInfo>
<Affiliation>National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Chu</LastName>
<ForeName>Daniel Kw</ForeName>
<Initials>DK</Initials>
<AffiliationInfo>
<Affiliation>University of Hong Kong, Hong Kong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Bleicker</LastName>
<ForeName>Tobias</ForeName>
<Initials>T</Initials>
<AffiliationInfo>
<Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Brünink</LastName>
<ForeName>Sebastian</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Schneider</LastName>
<ForeName>Julia</ForeName>
<Initials>J</Initials>
<AffiliationInfo>
<Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Schmidt</LastName>
<ForeName>Marie Luisa</ForeName>
<Initials>ML</Initials>
<AffiliationInfo>
<Affiliation>Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
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