Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Identifieur interne : 000640 ( PubMed/Curation ); précédent : 000639; suivant : 000641Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Auteurs : Shutoku Matsuyama [Japon] ; Naganori Nao [Japon] ; Kazuya Shirato [Japon] ; Miyuki Kawase [Japon] ; Shinji Saito [Japon] ; Ikuyo Takayama [Japon] ; Noriyo Nagata [Japon] ; Tsuyoshi Sekizuka [Japon] ; Hiroshi Katoh [Japon] ; Fumihiro Kato [Japon] ; Masafumi Sakata [Japon] ; Maino Tahara [Japon] ; Satoshi Kutsuna [Japon] ; Norio Ohmagari [Japon] ; Makoto Kuroda [Japon] ; Tadaki Suzuki [Japon] ; Tsutomu Kageyama [Japon] ; Makoto Takeda [Japon]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN viral, Serine endopeptidases.
- virologie : Infections à coronavirus, Pneumopathie virale.
- Animaux, Cellules Vero, Culture virale, Flambées de maladies, Humains, Lignée cellulaire, Pandémies.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : RNA, Viral, Serine Endopeptidases.
- isolation & purification : Betacoronavirus.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Animals, Cell Line, Chlorocebus aethiops, Disease Outbreaks, Humans, Pandemics, Vero Cells, Virus Cultivation.
Abstract
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
DOI: 10.1073/pnas.2002589117
PubMed: 32165541
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pubmed:32165541Le document en format XML
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<author><name sortKey="Sekizuka, Tsuyoshi" sort="Sekizuka, Tsuyoshi" uniqKey="Sekizuka T" first="Tsuyoshi" last="Sekizuka">Tsuyoshi Sekizuka</name>
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<author><name sortKey="Sakata, Masafumi" sort="Sakata, Masafumi" uniqKey="Sakata M" first="Masafumi" last="Sakata">Masafumi Sakata</name>
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<author><name sortKey="Tahara, Maino" sort="Tahara, Maino" uniqKey="Tahara M" first="Maino" last="Tahara">Maino Tahara</name>
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<author><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name>
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<author><name sortKey="Takeda, Makoto" sort="Takeda, Makoto" uniqKey="Takeda M" first="Makoto" last="Takeda">Makoto Takeda</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.</title>
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<author><name sortKey="Sekizuka, Tsuyoshi" sort="Sekizuka, Tsuyoshi" uniqKey="Sekizuka T" first="Tsuyoshi" last="Sekizuka">Tsuyoshi Sekizuka</name>
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<author><name sortKey="Katoh, Hiroshi" sort="Katoh, Hiroshi" uniqKey="Katoh H" first="Hiroshi" last="Katoh">Hiroshi Katoh</name>
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<author><name sortKey="Kato, Fumihiro" sort="Kato, Fumihiro" uniqKey="Kato F" first="Fumihiro" last="Kato">Fumihiro Kato</name>
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<author><name sortKey="Sakata, Masafumi" sort="Sakata, Masafumi" uniqKey="Sakata M" first="Masafumi" last="Sakata">Masafumi Sakata</name>
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<author><name sortKey="Tahara, Maino" sort="Tahara, Maino" uniqKey="Tahara M" first="Maino" last="Tahara">Maino Tahara</name>
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<author><name sortKey="Kutsuna, Satoshi" sort="Kutsuna, Satoshi" uniqKey="Kutsuna S" first="Satoshi" last="Kutsuna">Satoshi Kutsuna</name>
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<author><name sortKey="Ohmagari, Norio" sort="Ohmagari, Norio" uniqKey="Ohmagari N" first="Norio" last="Ohmagari">Norio Ohmagari</name>
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<author><name sortKey="Kuroda, Makoto" sort="Kuroda, Makoto" uniqKey="Kuroda M" first="Makoto" last="Kuroda">Makoto Kuroda</name>
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<author><name sortKey="Suzuki, Tadaki" sort="Suzuki, Tadaki" uniqKey="Suzuki T" first="Tadaki" last="Suzuki">Tadaki Suzuki</name>
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<author><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name>
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<author><name sortKey="Takeda, Makoto" sort="Takeda, Makoto" uniqKey="Takeda M" first="Makoto" last="Takeda">Makoto Takeda</name>
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<term>Betacoronavirus (isolation & purification)</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus Infections (virology)</term>
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>Pandemics</term>
<term>Pneumonia, Viral (virology)</term>
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<term>Cellules Vero</term>
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<front><div type="abstract" xml:lang="en">A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.</div>
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<Month>03</Month>
<Day>31</Day>
</PubDate>
</JournalIssue>
<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
<ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation>
</Journal>
<ArticleTitle>Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.</ArticleTitle>
<Pagination><MedlinePgn>7001-7003</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.2002589117</ELocationID>
<Abstract><AbstractText>A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.</AbstractText>
<CopyrightInformation>Copyright © 2020 the Author(s). Published by PNAS.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Matsuyama</LastName>
<ForeName>Shutoku</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nao</LastName>
<ForeName>Naganori</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shirato</LastName>
<ForeName>Kazuya</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kawase</LastName>
<ForeName>Miyuki</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Saito</LastName>
<ForeName>Shinji</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Takayama</LastName>
<ForeName>Ikuyo</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nagata</LastName>
<ForeName>Noriyo</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sekizuka</LastName>
<ForeName>Tsuyoshi</ForeName>
<Initials>T</Initials>
<Identifier Source="ORCID">0000-0002-1302-5472</Identifier>
<AffiliationInfo><Affiliation>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Katoh</LastName>
<ForeName>Hiroshi</ForeName>
<Initials>H</Initials>
<Identifier Source="ORCID">0000-0002-8613-4717</Identifier>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kato</LastName>
<ForeName>Fumihiro</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sakata</LastName>
<ForeName>Masafumi</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tahara</LastName>
<ForeName>Maino</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kutsuna</LastName>
<ForeName>Satoshi</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ohmagari</LastName>
<ForeName>Norio</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kuroda</LastName>
<ForeName>Makoto</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0002-0487-4405</Identifier>
<AffiliationInfo><Affiliation>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Suzuki</LastName>
<ForeName>Tadaki</ForeName>
<Initials>T</Initials>
<Identifier Source="ORCID">0000-0002-3820-9542</Identifier>
<AffiliationInfo><Affiliation>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kageyama</LastName>
<ForeName>Tsutomu</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Takeda</LastName>
<ForeName>Makoto</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">0000-0002-8194-7727</Identifier>
<AffiliationInfo><Affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>03</Month>
<Day>12</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Proc Natl Acad Sci U S A</MedlineTA>
<NlmUniqueID>7505876</NlmUniqueID>
<ISSNLinking>0027-8424</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.21.-</RegistryNumber>
<NameOfSubstance UI="D012697">Serine Endopeptidases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.21.-</RegistryNumber>
<NameOfSubstance UI="C421305">TMPRSS2 protein, human</NameOfSubstance>
</Chemical>
</ChemicalList>
<SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName>
<SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName>
</SupplMeshList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012697" MajorTopicYN="N">Serine Endopeptidases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014776" MajorTopicYN="N">Virus Cultivation</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword>
<Keyword MajorTopicYN="Y">TMPRSS2</Keyword>
<Keyword MajorTopicYN="Y">VeroE6</Keyword>
<Keyword MajorTopicYN="Y">coronavirus</Keyword>
<Keyword MajorTopicYN="Y">outbreak</Keyword>
</KeywordList>
<CoiStatement>The authors declare no competing interest.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>3</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>4</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>3</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32165541</ArticleId>
<ArticleId IdType="pii">2002589117</ArticleId>
<ArticleId IdType="doi">10.1073/pnas.2002589117</ArticleId>
<ArticleId IdType="pmc">PMC7132130</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):270-273</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32015507</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2020 Feb 22;395(10224):565-574</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32007145</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2018 Sep 12;92(19):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30021905</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1691-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690935</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11876-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16081529</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2010 Dec;84(24):12658-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20926566</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Arch Virol. 2007;152(5):1019-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17277901</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2011 Jan;85(2):873-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21068237</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Dec;87(23):12552-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24027332</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2019 Mar 5;93(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30626688</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Jpn J Infect Dis. 2020 Feb 18;:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32074516</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2020 Feb 20;382(8):727-733</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31978945</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 May;87(10):5502-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23468491</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):265-269</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32015508</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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