SrasV1, PubMed, Corpus, bibRecord, 000640

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

Identifieur interne : 000640 ( PubMed/Corpus ); précédent : 000639; suivant : 000641

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

Auteurs : Shutoku Matsuyama ; Naganori Nao ; Kazuya Shirato ; Miyuki Kawase ; Shinji Saito ; Ikuyo Takayama ; Noriyo Nagata ; Tsuyoshi Sekizuka ; Hiroshi Katoh ; Fumihiro Kato ; Masafumi Sakata ; Maino Tahara ; Satoshi Kutsuna ; Norio Ohmagari ; Makoto Kuroda ; Tadaki Suzuki ; Tsutomu Kageyama ; Makoto Takeda

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.

RBID : pubmed:32165541

English descriptors

KwdEn :
- Animals, Betacoronavirus (isolation & purification), Cell Line, Chlorocebus aethiops, Coronavirus Infections (virology), Disease Outbreaks, Humans, Pandemics, Pneumonia, Viral (virology), RNA, Viral (metabolism), Serine Endopeptidases (metabolism), Vero Cells, Virus Cultivation.
MESH :
- chemical , metabolism : RNA, Viral, Serine Endopeptidases.
- isolation & purification : Betacoronavirus.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Animals, Cell Line, Chlorocebus aethiops, Disease Outbreaks, Humans, Pandemics, Vero Cells, Virus Cultivation.

Abstract

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

DOI: 10.1073/pnas.2002589117
PubMed: 32165541

Links to Exploration step

pubmed:32165541

Le document en format XML

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<front><div type="abstract" xml:lang="en">A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.</div>
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<Abstract><AbstractText>A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.</AbstractText>
<CopyrightInformation>Copyright © 2020 the Author(s). Published by PNAS.</CopyrightInformation>
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<ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):270-273</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32015507</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2020 Feb 22;395(10224):565-574</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32007145</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2018 Sep 12;92(19):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30021905</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1691-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690935</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11876-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16081529</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2010 Dec;84(24):12658-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20926566</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Arch Virol. 2007;152(5):1019-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17277901</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2011 Jan;85(2):873-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21068237</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Dec;87(23):12552-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24027332</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2019 Mar 5;93(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30626688</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Jpn J Infect Dis. 2020 Feb 18;:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32074516</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2020 Feb 20;382(8):727-733</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31978945</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 May;87(10):5502-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23468491</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):265-269</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32015508</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

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