An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.
Identifieur interne : 002C66 ( PubMed/Corpus ); précédent : 002C65; suivant : 002C67An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.
Auteurs : Elisabetta Traggiai ; Stephan Becker ; Kanta Subbarao ; Larissa Kolesnikova ; Yasushi Uematsu ; Maria Rita Gismondo ; Brian R. Murphy ; Rino Rappuoli ; Antonio LanzavecchiaSource :
- Nature medicine [ 1078-8956 ] ; 2004.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Monoclonal (isolation & purification), B-Lymphocytes (immunology), Chlorocebus aethiops, DNA Primers, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Herpesvirus 4, Human (metabolism), Humans, Immunization, Passive (methods), Immunologic Memory (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, Microscopy, Immunoelectron, Neutralization Tests, Polymerase Chain Reaction, Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Transformation, Genetic, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), alpha-Macroglobulins (metabolism).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , isolation & purification : Antibodies, Monoclonal.
- immunology : B-Lymphocytes, Immunologic Memory, Severe Acute Respiratory Syndrome.
- metabolism : Herpesvirus 4, Human, Membrane Glycoproteins, Viral Envelope Proteins, alpha-Macroglobulins.
- methods : Immunization, Passive.
- Adult, Animals, Chlorocebus aethiops, DNA Primers, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Microscopy, Immunoelectron, Neutralization Tests, Polymerase Chain Reaction, Spike Glycoprotein, Coronavirus, Transformation, Genetic, Vero Cells.
Abstract
Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.
DOI: 10.1038/nm1080
PubMed: 15247913
Links to Exploration step
pubmed:15247913Le document en format XML
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Murphy</name></author><author><name sortKey="Rappuoli, Rino" sort="Rappuoli, Rino" uniqKey="Rappuoli R" first="Rino" last="Rappuoli">Rino Rappuoli</name></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15247913</idno><idno type="pmid">15247913</idno><idno type="doi">10.1038/nm1080</idno><idno type="wicri:Area/PubMed/Corpus">002C66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.</title><author><name sortKey="Traggiai, Elisabetta" sort="Traggiai, Elisabetta" uniqKey="Traggiai E" first="Elisabetta" last="Traggiai">Elisabetta Traggiai</name><affiliation><nlm:affiliation>Institute for Research in Biomedicine, Via Vela 6, CH 6500 Belllinzona, Switzerland.</nlm:affiliation></affiliation></author><author><name sortKey="Becker, Stephan" sort="Becker, Stephan" uniqKey="Becker S" first="Stephan" last="Becker">Stephan Becker</name></author><author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name></author><author><name sortKey="Kolesnikova, Larissa" sort="Kolesnikova, Larissa" uniqKey="Kolesnikova L" first="Larissa" last="Kolesnikova">Larissa Kolesnikova</name></author><author><name sortKey="Uematsu, Yasushi" sort="Uematsu, Yasushi" uniqKey="Uematsu Y" first="Yasushi" last="Uematsu">Yasushi Uematsu</name></author><author><name sortKey="Gismondo, Maria Rita" sort="Gismondo, Maria Rita" uniqKey="Gismondo M" first="Maria Rita" last="Gismondo">Maria Rita Gismondo</name></author><author><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R" last="Murphy">Brian R. Murphy</name></author><author><name sortKey="Rappuoli, Rino" sort="Rappuoli, Rino" uniqKey="Rappuoli R" first="Rino" last="Rappuoli">Rino Rappuoli</name></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name></author></analytic><series><title level="j">Nature medicine</title><idno type="ISSN">1078-8956</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies, Monoclonal (isolation & purification)</term><term>B-Lymphocytes (immunology)</term><term>Chlorocebus aethiops</term><term>DNA Primers</term><term>Disease Models, Animal</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Herpesvirus 4, Human (metabolism)</term><term>Humans</term><term>Immunization, Passive (methods)</term><term>Immunologic Memory (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Microscopy, Immunoelectron</term><term>Neutralization Tests</term><term>Polymerase Chain Reaction</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Transformation, Genetic</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term><term>alpha-Macroglobulins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Immunologic Memory</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Herpesvirus 4, Human</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>alpha-Macroglobulins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Passive</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Chlorocebus aethiops</term><term>DNA Primers</term><term>Disease Models, Animal</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Mice</term><term>Microscopy, Immunoelectron</term><term>Neutralization Tests</term><term>Polymerase Chain Reaction</term><term>Spike Glycoprotein, Coronavirus</term><term>Transformation, Genetic</term><term>Vero Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15247913</PMID><DateCompleted><Year>2004</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1078-8956</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>8</Issue><PubDate><Year>2004</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Nature medicine</Title><ISOAbbreviation>Nat. Med.</ISOAbbreviation></Journal><ArticleTitle>An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus.</ArticleTitle><Pagination><MedlinePgn>871-5</MedlinePgn></Pagination><Abstract><AbstractText>Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Traggiai</LastName><ForeName>Elisabetta</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute for Research in Biomedicine, Via Vela 6, CH 6500 Belllinzona, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Becker</LastName><ForeName>Stephan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Subbarao</LastName><ForeName>Kanta</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kolesnikova</LastName><ForeName>Larissa</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Uematsu</LastName><ForeName>Yasushi</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Gismondo</LastName><ForeName>Maria Rita</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Murphy</LastName><ForeName>Brian R</ForeName><Initials>BR</Initials></Author><Author ValidYN="Y"><LastName>Rappuoli</LastName><ForeName>Rino</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Lanzavecchia</LastName><ForeName>Antonio</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>07</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Nat 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