SARS antibody test for serosurveillance.
Identifieur interne : 002A95 ( PubMed/Corpus ); précédent : 002A94; suivant : 002A96SARS antibody test for serosurveillance.
Auteurs : Po-Ren Hsueh ; Chuan-Liang Kao ; Chun-Nan Lee ; Li-Kuan Chen ; Mei-Shang Ho ; Charles Sia ; Xin De Fang ; Shugene Lynn ; Tseng Yyuan Chang ; Shi Kau Liu ; Alan M. Walfield ; Chang Yi WangSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Disease Outbreaks, Enzyme-Linked Immunosorbent Assay (methods), Humans, Population Surveillance (methods), Retrospective Studies, SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology).
- MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay, Population Surveillance.
- Disease Outbreaks, Humans, Retrospective Studies, Sensitivity and Specificity.
Abstract
A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.
DOI: 10.3201/eid1009.040101
PubMed: 15498156
Links to Exploration step
pubmed:15498156Le document en format XML
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Walfield</name></author><author><name sortKey="Wang, Chang Yi" sort="Wang, Chang Yi" uniqKey="Wang C" first="Chang Yi" last="Wang">Chang Yi Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15498156</idno><idno type="pmid">15498156</idno><idno type="doi">10.3201/eid1009.040101</idno><idno type="wicri:Area/PubMed/Corpus">002A95</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS antibody test for serosurveillance.</title><author><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name><affiliation><nlm:affiliation>National Taiwan University College of Medicine, Taipei, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name></author><author><name sortKey="Lee, Chun Nan" sort="Lee, Chun Nan" uniqKey="Lee C" first="Chun-Nan" last="Lee">Chun-Nan Lee</name></author><author><name sortKey="Chen, Li Kuan" sort="Chen, Li Kuan" uniqKey="Chen L" first="Li-Kuan" last="Chen">Li-Kuan Chen</name></author><author><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name></author><author><name sortKey="Sia, Charles" sort="Sia, Charles" uniqKey="Sia C" first="Charles" last="Sia">Charles Sia</name></author><author><name sortKey="Fang, Xin De" sort="Fang, Xin De" uniqKey="Fang X" first="Xin De" last="Fang">Xin De Fang</name></author><author><name sortKey="Lynn, Shugene" sort="Lynn, Shugene" uniqKey="Lynn S" first="Shugene" last="Lynn">Shugene Lynn</name></author><author><name sortKey="Chang, Tseng Yyuan" sort="Chang, Tseng Yyuan" uniqKey="Chang T" first="Tseng Yyuan" last="Chang">Tseng Yyuan Chang</name></author><author><name sortKey="Liu, Shi Kau" sort="Liu, Shi Kau" uniqKey="Liu S" first="Shi Kau" last="Liu">Shi Kau Liu</name></author><author><name sortKey="Walfield, Alan M" sort="Walfield, Alan M" uniqKey="Walfield A" first="Alan M" last="Walfield">Alan M. Walfield</name></author><author><name sortKey="Wang, Chang Yi" sort="Wang, Chang Yi" uniqKey="Wang C" first="Chang Yi" last="Wang">Chang Yi Wang</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Disease Outbreaks</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Population Surveillance (methods)</term><term>Retrospective Studies</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Population Surveillance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>Humans</term><term>Retrospective Studies</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15498156</PMID><DateCompleted><Year>2004</Year><Month>12</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1080-6040</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>9</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Emerging infectious diseases</Title><ISOAbbreviation>Emerging Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>SARS antibody test for serosurveillance.</ArticleTitle><Pagination><MedlinePgn>1558-62</MedlinePgn></Pagination><Abstract><AbstractText>A peptide-based enzyme-linked immunosorbent assay (ELISA) can be used for retrospective serosurveillance of severe acute respiratory syndrome (SARS) by helping identify undetected chains of disease transmission. The assay was developed by epitope mapping, using synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-associated coronavirus. The new peptide ELISA consistently detected seroconversion by week 2 of onset of fever, and seropositivity remained through day 100. Specificity was 100% on normal blood donor samples, on serum samples associated with infection by other pathogens, and on an interference panel. The peptide-based test has advantages of safety, standardization, and automation over previous immunoassays for SARS. The assay was used for a retrospective survey of healthy healthcare workers in Taiwan who treated SARS patients. Asymptomatic seroconversions were detected in two hospitals that had nosocomial disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hsueh</LastName><ForeName>Po-Ren</ForeName><Initials>PR</Initials><AffiliationInfo><Affiliation>National Taiwan University College of Medicine, Taipei, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kao</LastName><ForeName>Chuan-Liang</ForeName><Initials>CL</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Chun-Nan</ForeName><Initials>CN</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Li-Kuan</ForeName><Initials>LK</Initials></Author><Author ValidYN="Y"><LastName>Ho</LastName><ForeName>Mei-Shang</ForeName><Initials>MS</Initials></Author><Author ValidYN="Y"><LastName>Sia</LastName><ForeName>Charles</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Xin De</ForeName><Initials>XD</Initials></Author><Author ValidYN="Y"><LastName>Lynn</LastName><ForeName>Shugene</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Tseng Yyuan</ForeName><Initials>TY</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Shi Kau</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>Walfield</LastName><ForeName>Alan M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Chang Yi</ForeName><Initials>CY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Infect Dis</MedlineTA><NlmUniqueID>9508155</NlmUniqueID><ISSNLinking>1080-6040</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011159" MajorTopicYN="N">Population Surveillance</DescriptorName><QualifierName UI="Q000379" 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