Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.
Identifieur interne : 002A15 ( PubMed/Corpus ); précédent : 002A14; suivant : 002A16Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.
Auteurs : Ping Zhao ; Jie Cao ; Lan-Juan Zhao ; Zhao-Lin Qin ; Jin-Shan Ke ; Wei Pan ; Hao Ren ; Jian-Guo Yu ; Zhong-Tian QiSource :
- Virology [ 0042-6822 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), Antibodies, Viral (blood), COS Cells, Cell Division (immunology), Cell Line, Tumor, Chlorocebus aethiops, Cytokines (metabolism), Escherichia coli, Female, Hypersensitivity, Delayed (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Spleen (cytology), Spleen (immunology), T-Lymphocytes, Cytotoxic (immunology), Vaccines, DNA (adverse effects), Vaccines, DNA (immunology), Viral Vaccines (adverse effects), Viral Vaccines (immunology).
- MESH :
- chemical , adverse effects : Vaccines, DNA, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Viral.
- chemical , blood : Antibodies, Viral.
- cytology : Spleen.
- immunology : Cell Division, Hypersensitivity, Delayed, Nucleocapsid Proteins, Recombinant Proteins, SARS Virus, Spleen, T-Lymphocytes, Cytotoxic, Vaccines, DNA, Viral Vaccines.
- chemical , metabolism : Cytokines.
- Animals, COS Cells, Cell Line, Tumor, Chlorocebus aethiops, Escherichia coli, Female, Mice, Mice, Inbred BALB C.
Abstract
The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DH5alpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobulins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity (DTH) and CD8(+) CTL responses to N protein. The study shows that N protein of SARS-CoV not only is an important B cell immunogen, but also can elicit broad-based cellular immune responses. The results indicate that the N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS.
DOI: 10.1016/j.virol.2004.10.016
PubMed: 15582659
Links to Exploration step
pubmed:15582659Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.</title><author><name sortKey="Zhao, Ping" sort="Zhao, Ping" uniqKey="Zhao P" first="Ping" last="Zhao">Ping Zhao</name><affiliation><nlm:affiliation>Department of Microbiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name></author><author><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></author><author><name sortKey="Qin, Zhao Lin" sort="Qin, Zhao Lin" uniqKey="Qin Z" first="Zhao-Lin" last="Qin">Zhao-Lin Qin</name></author><author><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name></author><author><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name></author><author><name sortKey="Ren, Hao" sort="Ren, Hao" uniqKey="Ren H" first="Hao" last="Ren">Hao Ren</name></author><author><name sortKey="Yu, Jian Guo" sort="Yu, Jian Guo" uniqKey="Yu J" first="Jian-Guo" last="Yu">Jian-Guo Yu</name></author><author><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15582659</idno><idno type="pmid">15582659</idno><idno type="doi">10.1016/j.virol.2004.10.016</idno><idno type="wicri:Area/PubMed/Corpus">002A15</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A15</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.</title><author><name sortKey="Zhao, Ping" sort="Zhao, Ping" uniqKey="Zhao P" first="Ping" last="Zhao">Ping Zhao</name><affiliation><nlm:affiliation>Department of Microbiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name></author><author><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></author><author><name sortKey="Qin, Zhao Lin" sort="Qin, Zhao Lin" uniqKey="Qin Z" first="Zhao-Lin" last="Qin">Zhao-Lin Qin</name></author><author><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name></author><author><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name></author><author><name sortKey="Ren, Hao" sort="Ren, Hao" uniqKey="Ren H" first="Hao" last="Ren">Hao Ren</name></author><author><name sortKey="Yu, Jian Guo" sort="Yu, Jian Guo" uniqKey="Yu J" first="Jian-Guo" last="Yu">Jian-Guo Yu</name></author><author><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (blood)</term><term>COS Cells</term><term>Cell Division (immunology)</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Cytokines (metabolism)</term><term>Escherichia coli</term><term>Female</term><term>Hypersensitivity, Delayed (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Spleen (cytology)</term><term>Spleen (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Vaccines, DNA (adverse effects)</term><term>Vaccines, DNA (immunology)</term><term>Viral Vaccines (adverse effects)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cell Division</term><term>Hypersensitivity, Delayed</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Spleen</term><term>T-Lymphocytes, Cytotoxic</term><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Escherichia coli</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DH5alpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobulins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity (DTH) and CD8(+) CTL responses to N protein. The study shows that N protein of SARS-CoV not only is an important B cell immunogen, but also can elicit broad-based cellular immune responses. The results indicate that the N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15582659</PMID><DateCompleted><Year>2005</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>331</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month><Day>05</Day></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine.</ArticleTitle><Pagination><MedlinePgn>128-35</MedlinePgn></Pagination><Abstract><AbstractText>The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DH5alpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobulins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity (DTH) and CD8(+) CTL responses to N protein. The study shows that N protein of SARS-CoV not only is an important B cell immunogen, but also can elicit broad-based cellular immune responses. The results indicate that the N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Lan-Juan</ForeName><Initials>LJ</Initials></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Zhao-Lin</ForeName><Initials>ZL</Initials></Author><Author ValidYN="Y"><LastName>Ke</LastName><ForeName>Jin-Shan</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Hao</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Jian-Guo</ForeName><Initials>JG</Initials></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Zhong-Tian</ForeName><Initials>ZT</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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