[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].
Identifieur interne : 002911 ( PubMed/Corpus ); précédent : 002910; suivant : 002912[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].
Auteurs : Jian-Na Li ; Kai-Jun Xiang ; Rong Zhou ; Chun-Hua Huang ; Yong-Qiang Ding ; Qi-Yi Zeng ; Qiu-Ping ZhongSource :
- Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [ 1000-2588 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Escherichia coli (genetics), Escherichia coli (metabolism), Genetic Vectors, Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Prokaryotic Cells (metabolism), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (genetics), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vaccines, DNA (biosynthesis), Vaccines, DNA (immunology), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (biosynthesis), Viral Vaccines (immunology).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- genetics : Escherichia coli, Membrane Glycoproteins, Recombinant Proteins, SARS Virus, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- metabolism : Escherichia coli, Prokaryotic Cells.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Genetic Vectors, Humans, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
To study the immunological characteristics of the spike (S) protein of SARS coronavirus (SARS-CoV) and analyze the feasibility of using this protein as the component for SARS vaccine development.
PubMed: 15683993
Links to Exploration step
pubmed:15683993Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].</title><author><name sortKey="Li, Jian Na" sort="Li, Jian Na" uniqKey="Li J" first="Jian-Na" last="Li">Jian-Na Li</name><affiliation><nlm:affiliation>Southern China University of Tropical Agriculture, Danzhou 571737, China. christina0125@163.com</nlm:affiliation></affiliation></author><author><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name></author><author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name></author><author><name sortKey="Huang, Chun Hua" sort="Huang, Chun Hua" uniqKey="Huang C" first="Chun-Hua" last="Huang">Chun-Hua Huang</name></author><author><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name></author><author><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name></author><author><name sortKey="Zhong, Qiu Ping" sort="Zhong, Qiu Ping" uniqKey="Zhong Q" first="Qiu-Ping" last="Zhong">Qiu-Ping Zhong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15683993</idno><idno type="pmid">15683993</idno><idno type="wicri:Area/PubMed/Corpus">002911</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002911</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].</title><author><name sortKey="Li, Jian Na" sort="Li, Jian Na" uniqKey="Li J" first="Jian-Na" last="Li">Jian-Na Li</name><affiliation><nlm:affiliation>Southern China University of Tropical Agriculture, Danzhou 571737, China. christina0125@163.com</nlm:affiliation></affiliation></author><author><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name></author><author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name></author><author><name sortKey="Huang, Chun Hua" sort="Huang, Chun Hua" uniqKey="Huang C" first="Chun-Hua" last="Huang">Chun-Hua Huang</name></author><author><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name></author><author><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name></author><author><name sortKey="Zhong, Qiu Ping" sort="Zhong, Qiu Ping" uniqKey="Zhong Q" first="Qiu-Ping" last="Zhong">Qiu-Ping Zhong</name></author></analytic><series><title level="j">Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA</title><idno type="ISSN">1000-2588</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Genetic Vectors</term><term>Humans</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Prokaryotic Cells (metabolism)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccines, DNA (biosynthesis)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (biosynthesis)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Prokaryotic Cells</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genetic Vectors</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To study the immunological characteristics of the spike (S) protein of SARS coronavirus (SARS-CoV) and analyze the feasibility of using this protein as the component for SARS vaccine development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15683993</PMID><DateCompleted><Year>2007</Year><Month>05</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-2588</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA</Title><ISOAbbreviation>Di Yi Jun Yi Da Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].</ArticleTitle><Pagination><MedlinePgn>33-6</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study the immunological characteristics of the spike (S) protein of SARS coronavirus (SARS-CoV) and analyze the feasibility of using this protein as the component for SARS vaccine development.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The two truncated fragments of S gene were separately cloned into the prokaryotic expression vector pET-15b and expressed in E.coli. The resulting recombinant proteins, rS(a) and rS(b), were purified by affinity chromatography. The full-length S gene was cloned into the eukaryotic expression plasmid pSecTagB to prepare recombinant plasmid pSecS as the DNA vaccine to immunize BALB/c mice for inducing the secretion of anti-SARS-CoV protein. The immunological effect of anti-SARS-CoV antibody was tested with purified rS(a) and rS(b) proteins by enzyme-linked immunosorbent assay (ELISA).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Both the truncated recombinant proteins were expressed in soluble forms and reacted specifically with the sera from immunized pSecS mice and clinically diagnosed SARS patients. The prokaryotically expressed recombinant truncated S protein had similar antigenicity with SARS-CoV S protein.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The recombinant protein could be used as an antigen for detecting the serum of SARS CoV-infected patients. The SARS-CoV S gene vaccine could induce the production of specific antibody, which offers clues for the research of SARS DNA vaccine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jian-na</ForeName><Initials>JN</Initials><AffiliationInfo><Affiliation>Southern China University of Tropical Agriculture, Danzhou 571737, China. christina0125@163.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Kai-jun</ForeName><Initials>KJ</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Rong</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Chun-hua</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Yong-qiang</ForeName><Initials>YQ</Initials></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Qi-yi</ForeName><Initials>QY</Initials></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Qiu-ping</ForeName><Initials>QP</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Di Yi Jun Yi Da Xue Xue Bao</MedlineTA><NlmUniqueID>9426110</NlmUniqueID><ISSNLinking>1000-2588</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011387" MajorTopicYN="N">Prokaryotic Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>2</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>5</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>2</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15683993</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002911 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002911 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15683993
|texte= [Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15683993" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |