The protein X4 of severe acute respiratory syndrome-associated coronavirus is expressed on both virus-infected cells and lung tissue of severe acute respiratory syndrome patients and inhibits growth of Balb/c 3T3 cell line.
Identifieur interne : 002866 ( PubMed/Corpus ); précédent : 002865; suivant : 002867The protein X4 of severe acute respiratory syndrome-associated coronavirus is expressed on both virus-infected cells and lung tissue of severe acute respiratory syndrome patients and inhibits growth of Balb/c 3T3 cell line.
Auteurs : Ying-Yu Chen ; Bao Shuang ; Ya-Xia Tan ; Min-Jie Meng ; Pu Han ; Xiao-Ning Mo ; Quan-Sheng Song ; Xiao-Yan Qiu ; Xin Luo ; Qi-Ni Gan ; Xin Zhang ; Ying Zheng ; Shun-Ai Liu ; Xiao-Ning Wang ; Nan-Shan Zhong ; Da-Long MaSource :
- Chinese medical journal [ 0366-6999 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, BALB 3T3 Cells, Chlorocebus aethiops, Growth Inhibitors (analysis), Growth Inhibitors (physiology), HeLa Cells, Humans, Immunohistochemistry, Lung (chemistry), Mice, Molecular Sequence Data, SARS Virus (chemistry), Severe Acute Respiratory Syndrome (metabolism), Vero Cells, Viral Structural Proteins (analysis), Viral Structural Proteins (physiology).
- MESH :
- chemical , analysis : Growth Inhibitors, Viral Structural Proteins.
- chemical , physiology : Growth Inhibitors, Viral Structural Proteins.
- chemistry : Lung, SARS Virus.
- metabolism : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, BALB 3T3 Cells, Chlorocebus aethiops, HeLa Cells, Humans, Immunohistochemistry, Mice, Molecular Sequence Data, Vero Cells.
Abstract
The genome of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) includes sequences encoding the putative protein X4 (ORF8, ORF7a), consisting of 122 amino acids. The deduced sequence contains a probable cleaved signal peptide sequence and a C-terminal transmembrane helix, indicating that protein X4 is likely to be a type I membrane protein. This study was conducted to demonstrate whether the protein X4 was expressed and its essential function in the process of SARS-CoV infection.
PubMed: 15740663
Links to Exploration step
pubmed:15740663Le document en format XML
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<author><name sortKey="Chen, Ying Yu" sort="Chen, Ying Yu" uniqKey="Chen Y" first="Ying-Yu" last="Chen">Ying-Yu Chen</name>
<affiliation><nlm:affiliation>Center for Human Disease Genomics, Peking University, Beijing 100083, China.</nlm:affiliation>
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<author><name sortKey="Shuang, Bao" sort="Shuang, Bao" uniqKey="Shuang B" first="Bao" last="Shuang">Bao Shuang</name>
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<author><name sortKey="Tan, Ya Xia" sort="Tan, Ya Xia" uniqKey="Tan Y" first="Ya-Xia" last="Tan">Ya-Xia Tan</name>
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<author><name sortKey="Meng, Min Jie" sort="Meng, Min Jie" uniqKey="Meng M" first="Min-Jie" last="Meng">Min-Jie Meng</name>
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<author><name sortKey="Han, Pu" sort="Han, Pu" uniqKey="Han P" first="Pu" last="Han">Pu Han</name>
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<author><name sortKey="Mo, Xiao Ning" sort="Mo, Xiao Ning" uniqKey="Mo X" first="Xiao-Ning" last="Mo">Xiao-Ning Mo</name>
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<author><name sortKey="Song, Quan Sheng" sort="Song, Quan Sheng" uniqKey="Song Q" first="Quan-Sheng" last="Song">Quan-Sheng Song</name>
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<author><name sortKey="Qiu, Xiao Yan" sort="Qiu, Xiao Yan" uniqKey="Qiu X" first="Xiao-Yan" last="Qiu">Xiao-Yan Qiu</name>
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<author><name sortKey="Luo, Xin" sort="Luo, Xin" uniqKey="Luo X" first="Xin" last="Luo">Xin Luo</name>
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<author><name sortKey="Gan, Qi Ni" sort="Gan, Qi Ni" uniqKey="Gan Q" first="Qi-Ni" last="Gan">Qi-Ni Gan</name>
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<author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name>
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<author><name sortKey="Zheng, Ying" sort="Zheng, Ying" uniqKey="Zheng Y" first="Ying" last="Zheng">Ying Zheng</name>
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<author><name sortKey="Liu, Shun Ai" sort="Liu, Shun Ai" uniqKey="Liu S" first="Shun-Ai" last="Liu">Shun-Ai Liu</name>
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<author><name sortKey="Wang, Xiao Ning" sort="Wang, Xiao Ning" uniqKey="Wang X" first="Xiao-Ning" last="Wang">Xiao-Ning Wang</name>
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<author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name>
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<author><name sortKey="Ma, Da Long" sort="Ma, Da Long" uniqKey="Ma D" first="Da-Long" last="Ma">Da-Long Ma</name>
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<author><name sortKey="Chen, Ying Yu" sort="Chen, Ying Yu" uniqKey="Chen Y" first="Ying-Yu" last="Chen">Ying-Yu Chen</name>
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<author><name sortKey="Mo, Xiao Ning" sort="Mo, Xiao Ning" uniqKey="Mo X" first="Xiao-Ning" last="Mo">Xiao-Ning Mo</name>
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<author><name sortKey="Song, Quan Sheng" sort="Song, Quan Sheng" uniqKey="Song Q" first="Quan-Sheng" last="Song">Quan-Sheng Song</name>
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<author><name sortKey="Qiu, Xiao Yan" sort="Qiu, Xiao Yan" uniqKey="Qiu X" first="Xiao-Yan" last="Qiu">Xiao-Yan Qiu</name>
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<author><name sortKey="Luo, Xin" sort="Luo, Xin" uniqKey="Luo X" first="Xin" last="Luo">Xin Luo</name>
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<author><name sortKey="Gan, Qi Ni" sort="Gan, Qi Ni" uniqKey="Gan Q" first="Qi-Ni" last="Gan">Qi-Ni Gan</name>
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<author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name>
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<author><name sortKey="Zheng, Ying" sort="Zheng, Ying" uniqKey="Zheng Y" first="Ying" last="Zheng">Ying Zheng</name>
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<author><name sortKey="Liu, Shun Ai" sort="Liu, Shun Ai" uniqKey="Liu S" first="Shun-Ai" last="Liu">Shun-Ai Liu</name>
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<author><name sortKey="Wang, Xiao Ning" sort="Wang, Xiao Ning" uniqKey="Wang X" first="Xiao-Ning" last="Wang">Xiao-Ning Wang</name>
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<author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name>
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<author><name sortKey="Ma, Da Long" sort="Ma, Da Long" uniqKey="Ma D" first="Da-Long" last="Ma">Da-Long Ma</name>
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<series><title level="j">Chinese medical journal</title>
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<term>Animals</term>
<term>BALB 3T3 Cells</term>
<term>Chlorocebus aethiops</term>
<term>Growth Inhibitors (analysis)</term>
<term>Growth Inhibitors (physiology)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Lung (chemistry)</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>SARS Virus (chemistry)</term>
<term>Severe Acute Respiratory Syndrome (metabolism)</term>
<term>Vero Cells</term>
<term>Viral Structural Proteins (analysis)</term>
<term>Viral Structural Proteins (physiology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Growth Inhibitors</term>
<term>Viral Structural Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Growth Inhibitors</term>
<term>Viral Structural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Lung</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>BALB 3T3 Cells</term>
<term>Chlorocebus aethiops</term>
<term>HeLa Cells</term>
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<front><div type="abstract" xml:lang="en">The genome of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) includes sequences encoding the putative protein X4 (ORF8, ORF7a), consisting of 122 amino acids. The deduced sequence contains a probable cleaved signal peptide sequence and a C-terminal transmembrane helix, indicating that protein X4 is likely to be a type I membrane protein. This study was conducted to demonstrate whether the protein X4 was expressed and its essential function in the process of SARS-CoV infection.</div>
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<DateCompleted><Year>2005</Year>
<Month>04</Month>
<Day>28</Day>
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<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
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<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Feb</Month>
<Day>20</Day>
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<Title>Chinese medical journal</Title>
<ISOAbbreviation>Chin. Med. J.</ISOAbbreviation>
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<ArticleTitle>The protein X4 of severe acute respiratory syndrome-associated coronavirus is expressed on both virus-infected cells and lung tissue of severe acute respiratory syndrome patients and inhibits growth of Balb/c 3T3 cell line.</ArticleTitle>
<Pagination><MedlinePgn>267-74</MedlinePgn>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The genome of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) includes sequences encoding the putative protein X4 (ORF8, ORF7a), consisting of 122 amino acids. The deduced sequence contains a probable cleaved signal peptide sequence and a C-terminal transmembrane helix, indicating that protein X4 is likely to be a type I membrane protein. This study was conducted to demonstrate whether the protein X4 was expressed and its essential function in the process of SARS-CoV infection.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The prokaryotic and eukaryotic protein X4-expressing plasmids were constructed. Recombinant soluble protein X4 was purified from E. coli using ion exchange chromatography, and the preparation was injected into chicken for rising specific polyclonal antibodies. The expression of protein X4 in SARS-CoV-infected Vero E6 cells and lung tissues from patients with SARS was performed using immunofluorescence assay and immunohistochemistry technique. The preliminary function of protein X4 was evaluated by treatment with and over-expression of protein X4 in cell lines. Western blot was employed to evaluate the expression of protein X4 in SARS-CoV particles.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">We expressed and purified soluble recombinant protein X4 from E.coli, and generated specific antibodies against protein X4. Western blot proved that the protein X4 was not assembled in the SARS-CoV particles. Indirect immunofluorescence assays revealed that the expression of protein X4 was detected at 8 hours after infection in SARS-CoV-infected Vero E6 cells. It was also detected in the lung tissues from patients with SARS. Treatment with and overexpression of protein X4 inhibited the growth of Balb/c 3T3 cells as determined by cell counting and MTT assays.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The results provide the evidence of protein X4 expression following SARS-CoV infection, and may facilitate further investigation of the immunopathological mechanism of SARS.</AbstractText>
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