rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens.
Identifieur interne : 002782 ( PubMed/Corpus ); précédent : 002781; suivant : 002783rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens.
Auteurs : Angus J. Macdonald ; Long Cao ; Yuxian He ; Qian Zhao ; Shibo Jiang ; Sara LustigmanSource :
- Vaccine [ 0264-410X ] ; 2005.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Animals, Antigens, Helminth (administration & dosage), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (pharmacology), HIV-1 (drug effects), HIV-1 (immunology), Helminth Proteins (pharmacology), Mice, Ovalbumin (chemistry), Ovalbumin (immunology), Recombinant Proteins (immunology), Recombinant Proteins (pharmacology), SARS Virus (drug effects), SARS Virus (immunology), Secretory Vesicles (metabolism), Th1 Cells (immunology), Th2 Cells (immunology), Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (immunology).
- MESH :
- chemical , administration & dosage : Antigens, Helminth, Vaccines, Synthetic.
- chemical , chemistry : Ovalbumin.
- chemical , genetics : Antigens, Helminth.
- chemical , immunology : Antigens, Helminth, Ovalbumin, Recombinant Proteins, Vaccines, Synthetic.
- chemical , pharmacology : Adjuvants, Immunologic, Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- drug effects : HIV-1, SARS Virus.
- immunology : HIV-1, SARS Virus, Th1 Cells, Th2 Cells.
- metabolism : Secretory Vesicles.
- Animals, Mice.
Abstract
We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.
DOI: 10.1016/j.vaccine.2005.01.098
PubMed: 15837368
Links to Exploration step
pubmed:15837368Le document en format XML
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<author><name sortKey="Cao, Long" sort="Cao, Long" uniqKey="Cao L" first="Long" last="Cao">Long Cao</name>
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<author><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name>
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<author><name sortKey="Zhao, Qian" sort="Zhao, Qian" uniqKey="Zhao Q" first="Qian" last="Zhao">Qian Zhao</name>
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<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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<author><name sortKey="Zhao, Qian" sort="Zhao, Qian" uniqKey="Zhao Q" first="Qian" last="Zhao">Qian Zhao</name>
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<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
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<term>Antigens, Helminth (administration & dosage)</term>
<term>Antigens, Helminth (genetics)</term>
<term>Antigens, Helminth (immunology)</term>
<term>Antigens, Helminth (pharmacology)</term>
<term>HIV-1 (drug effects)</term>
<term>HIV-1 (immunology)</term>
<term>Helminth Proteins (pharmacology)</term>
<term>Mice</term>
<term>Ovalbumin (chemistry)</term>
<term>Ovalbumin (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Recombinant Proteins (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (immunology)</term>
<term>Secretory Vesicles (metabolism)</term>
<term>Th1 Cells (immunology)</term>
<term>Th2 Cells (immunology)</term>
<term>Vaccines, Synthetic (administration & dosage)</term>
<term>Vaccines, Synthetic (immunology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Antigens, Helminth</term>
<term>Vaccines, Synthetic</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ovalbumin</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term>
<term>Ovalbumin</term>
<term>Recombinant Proteins</term>
<term>Vaccines, Synthetic</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term>
<term>Antigens, Helminth</term>
<term>Helminth Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>HIV-1</term>
<term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV-1</term>
<term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Secretory Vesicles</term>
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<term>Mice</term>
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<front><div type="abstract" xml:lang="en">We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.</div>
</front>
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<ArticleTitle>rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens.</ArticleTitle>
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<Abstract><AbstractText>We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.</AbstractText>
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