SrasV1, PubMed, Corpus, bibRecord, 002781

[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].

Identifieur interne : 002781 ( PubMed/Corpus ); précédent : 002780; suivant : 002782

[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].

Auteurs : Lei Wang ; Ying-Song Wu ; Yong-Ping Tang ; Ming Li

Source :

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [ 1000-2588 ] ; 2005.

RBID : pubmed:15837647

English descriptors

KwdEn :
- Antibodies, Monoclonal (immunology), Fluoroimmunoassay (methods), Humans, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (virology).
MESH :
- chemical , analysis : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal, Nucleocapsid Proteins.
- isolation & purification : SARS Virus.
- methods : Fluoroimmunoassay.
- virology : Severe Acute Respiratory Syndrome.
- Humans.

Abstract

To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA).

PubMed: 15837647

Links to Exploration step

pubmed:15837647

Le document en format XML

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<author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name>
<affiliation><nlm:affiliation>Institute of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Wu, Ying Song" sort="Wu, Ying Song" uniqKey="Wu Y" first="Ying-Song" last="Wu">Ying-Song Wu</name>
</author>
<author><name sortKey="Tang, Yong Ping" sort="Tang, Yong Ping" uniqKey="Tang Y" first="Yong-Ping" last="Tang">Yong-Ping Tang</name>
</author>
<author><name sortKey="Li, Ming" sort="Li, Ming" uniqKey="Li M" first="Ming" last="Li">Ming Li</name>
</author>
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<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].</title>
<author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name>
<affiliation><nlm:affiliation>Institute of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Wu, Ying Song" sort="Wu, Ying Song" uniqKey="Wu Y" first="Ying-Song" last="Wu">Ying-Song Wu</name>
</author>
<author><name sortKey="Tang, Yong Ping" sort="Tang, Yong Ping" uniqKey="Tang Y" first="Yong-Ping" last="Tang">Yong-Ping Tang</name>
</author>
<author><name sortKey="Li, Ming" sort="Li, Ming" uniqKey="Li M" first="Ming" last="Li">Ming Li</name>
</author>
</analytic>
<series><title level="j">Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA</title>
<idno type="ISSN">1000-2588</idno>
<imprint><date when="2005" type="published">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (immunology)</term>
<term>Fluoroimmunoassay (methods)</term>
<term>Humans</term>
<term>Nucleocapsid Proteins (analysis)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluoroimmunoassay</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
</keywords>
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<front><div type="abstract" xml:lang="en">To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA).</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15837647</PMID>
<DateCompleted><Year>2007</Year>
<Month>05</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2005</Year>
<Month>04</Month>
<Day>19</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-2588</ISSN>
<JournalIssue CitedMedium="Print"><Volume>25</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Apr</Month>
</PubDate>
</JournalIssue>
<Title>Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA</Title>
<ISOAbbreviation>Di Yi Jun Yi Da Xue Xue Bao</ISOAbbreviation>
</Journal>
<ArticleTitle>[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].</ArticleTitle>
<Pagination><MedlinePgn>429-31, 434</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA).</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit.</AbstractText>
<AbstractText Label="RESULT" NlmCategory="RESULTS">The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%;-6.2%;, and coefficient of variability between days of 5.3%;-9.6%;. The results of detection were consistent between ELISA and TRFIA.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Lei</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Institute of Tropical Medicine, Southern Medical University, Guangzhou 510515, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Ying-Song</ForeName>
<Initials>YS</Initials>
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<Author ValidYN="Y"><LastName>Tang</LastName>
<ForeName>Yong-Ping</ForeName>
<Initials>YP</Initials>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Ming</ForeName>
<Initials>M</Initials>
</Author>
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<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Di Yi Jun Yi Da Xue Xue Bao</MedlineTA>
<NlmUniqueID>9426110</NlmUniqueID>
<ISSNLinking>1000-2588</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015200" MajorTopicYN="Y">Fluoroimmunoassay</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>4</Month>
<Day>20</Day>
<Hour>9</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="medline"><Year>2007</Year>
<Month>5</Month>
<Day>2</Day>
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<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15837647</ArticleId>
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[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].

[Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki