[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Identifieur interne : 002678 ( PubMed/Corpus ); précédent : 002677; suivant : 002679[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Auteurs : Yan-Ping Yi ; Chu-Fang Li ; Yu-Ling Shi ; Lin-Hai Li ; Ping Li ; Wei Huang ; Sheng-Qi Wang ; Qing-Jun Ma ; Cheng CaoSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2003.
English descriptors
- KwdEn :
- Chromatography, Affinity, Chromatography, Ion Exchange, Enzyme-Linked Immunosorbent Assay, Escherichia coli (genetics), Escherichia coli (metabolism), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (isolation & purification), Nucleocapsid Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (metabolism), Viral Structural Proteins (genetics), Viral Structural Proteins (isolation & purification), Viral Structural Proteins (metabolism).
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Structural Proteins.
- genetics : Escherichia coli, SARS Virus.
- chemical , isolation & purification : Nucleocapsid Proteins, Viral Structural Proteins.
- metabolism : Escherichia coli, Nucleocapsid Proteins, SARS Virus, Viral Structural Proteins.
- Chromatography, Affinity, Chromatography, Ion Exchange, Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.
PubMed: 15969052
Links to Exploration step
pubmed:15969052Le document en format XML
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last="Huang">Wei Huang</name></author><author><name sortKey="Wang, Sheng Qi" sort="Wang, Sheng Qi" uniqKey="Wang S" first="Sheng-Qi" last="Wang">Sheng-Qi Wang</name></author><author><name sortKey="Ma, Qing Jun" sort="Ma, Qing Jun" uniqKey="Ma Q" first="Qing-Jun" last="Ma">Qing-Jun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:15969052</idno><idno type="pmid">15969052</idno><idno type="wicri:Area/PubMed/Corpus">002678</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002678</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].</title><author><name sortKey="Yi, Yan Ping" sort="Yi, Yan Ping" uniqKey="Yi Y" first="Yan-Ping" last="Yi">Yan-Ping Yi</name><affiliation><nlm:affiliation>Beijing Institute of Biotechnology, Beijing 100850, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Chu Fang" sort="Li, Chu Fang" uniqKey="Li C" first="Chu-Fang" last="Li">Chu-Fang Li</name></author><author><name sortKey="Shi, Yu Ling" sort="Shi, Yu Ling" uniqKey="Shi Y" first="Yu-Ling" last="Shi">Yu-Ling Shi</name></author><author><name sortKey="Li, Lin Hai" sort="Li, Lin Hai" uniqKey="Li L" first="Lin-Hai" last="Li">Lin-Hai Li</name></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Huang, Wei" sort="Huang, Wei" uniqKey="Huang W" first="Wei" last="Huang">Wei Huang</name></author><author><name sortKey="Wang, Sheng Qi" sort="Wang, Sheng Qi" uniqKey="Wang S" first="Sheng-Qi" last="Wang">Sheng-Qi Wang</name></author><author><name sortKey="Ma, Qing Jun" sort="Ma, Qing Jun" uniqKey="Ma Q" first="Qing-Jun" last="Ma">Qing-Jun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></analytic><series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title><idno type="ISSN">1000-3061</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, Affinity</term><term>Chromatography, Ion Exchange</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Viral Structural Proteins (genetics)</term><term>Viral Structural Proteins (isolation & purification)</term><term>Viral Structural Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Nucleocapsid Proteins</term><term>SARS Virus</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Affinity</term><term>Chromatography, Ion Exchange</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15969052</PMID><DateCompleted><Year>2010</Year><Month>04</Month><Day>29</Day></DateCompleted><DateRevised><Year>2005</Year><Month>06</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-3061</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>4</Issue><PubDate><Year>2003</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title><ISOAbbreviation>Sheng Wu Gong Cheng Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].</ArticleTitle><Pagination><MedlinePgn>392-6</MedlinePgn></Pagination><Abstract><AbstractText>Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Yan-Ping</ForeName><Initials>YP</Initials><AffiliationInfo><Affiliation>Beijing Institute of Biotechnology, Beijing 100850, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chu-Fang</ForeName><Initials>CF</Initials></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Yu-Ling</ForeName><Initials>YL</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Lin-Hai</ForeName><Initials>LH</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ping</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Sheng-Qi</ForeName><Initials>SQ</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Qing-Jun</ForeName><Initials>QJ</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Cheng</ForeName><Initials>C</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Sheng Wu Gong Cheng Xue Bao</MedlineTA><NlmUniqueID>9426463</NlmUniqueID><ISSNLinking>1000-3061</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015678">Viral Structural Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002852" MajorTopicYN="N">Chromatography, Ion Exchange</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015678" MajorTopicYN="N">Viral Structural Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>6</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>6</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15969052</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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