[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Identifieur interne : 002678 ( PubMed/Corpus ); précédent : 002677; suivant : 002679[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Auteurs : Yan-Ping Yi ; Chu-Fang Li ; Yu-Ling Shi ; Lin-Hai Li ; Ping Li ; Wei Huang ; Sheng-Qi Wang ; Qing-Jun Ma ; Cheng CaoSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2003.
English descriptors
- KwdEn :
- Chromatography, Affinity, Chromatography, Ion Exchange, Enzyme-Linked Immunosorbent Assay, Escherichia coli (genetics), Escherichia coli (metabolism), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (isolation & purification), Nucleocapsid Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (metabolism), Viral Structural Proteins (genetics), Viral Structural Proteins (isolation & purification), Viral Structural Proteins (metabolism).
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Structural Proteins.
- genetics : Escherichia coli, SARS Virus.
- chemical , isolation & purification : Nucleocapsid Proteins, Viral Structural Proteins.
- metabolism : Escherichia coli, Nucleocapsid Proteins, SARS Virus, Viral Structural Proteins.
- Chromatography, Affinity, Chromatography, Ion Exchange, Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.
PubMed: 15969052
Links to Exploration step
pubmed:15969052Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].</title>
<author><name sortKey="Yi, Yan Ping" sort="Yi, Yan Ping" uniqKey="Yi Y" first="Yan-Ping" last="Yi">Yan-Ping Yi</name>
<affiliation><nlm:affiliation>Beijing Institute of Biotechnology, Beijing 100850, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Li, Chu Fang" sort="Li, Chu Fang" uniqKey="Li C" first="Chu-Fang" last="Li">Chu-Fang Li</name>
</author>
<author><name sortKey="Shi, Yu Ling" sort="Shi, Yu Ling" uniqKey="Shi Y" first="Yu-Ling" last="Shi">Yu-Ling Shi</name>
</author>
<author><name sortKey="Li, Lin Hai" sort="Li, Lin Hai" uniqKey="Li L" first="Lin-Hai" last="Li">Lin-Hai Li</name>
</author>
<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
</author>
<author><name sortKey="Huang, Wei" sort="Huang, Wei" uniqKey="Huang W" first="Wei" last="Huang">Wei Huang</name>
</author>
<author><name sortKey="Wang, Sheng Qi" sort="Wang, Sheng Qi" uniqKey="Wang S" first="Sheng-Qi" last="Wang">Sheng-Qi Wang</name>
</author>
<author><name sortKey="Ma, Qing Jun" sort="Ma, Qing Jun" uniqKey="Ma Q" first="Qing-Jun" last="Ma">Qing-Jun Ma</name>
</author>
<author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].</title>
<author><name sortKey="Yi, Yan Ping" sort="Yi, Yan Ping" uniqKey="Yi Y" first="Yan-Ping" last="Yi">Yan-Ping Yi</name>
<affiliation><nlm:affiliation>Beijing Institute of Biotechnology, Beijing 100850, China.</nlm:affiliation>
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<author><name sortKey="Li, Chu Fang" sort="Li, Chu Fang" uniqKey="Li C" first="Chu-Fang" last="Li">Chu-Fang Li</name>
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<author><name sortKey="Shi, Yu Ling" sort="Shi, Yu Ling" uniqKey="Shi Y" first="Yu-Ling" last="Shi">Yu-Ling Shi</name>
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<author><name sortKey="Li, Lin Hai" sort="Li, Lin Hai" uniqKey="Li L" first="Lin-Hai" last="Li">Lin-Hai Li</name>
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<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
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<author><name sortKey="Huang, Wei" sort="Huang, Wei" uniqKey="Huang W" first="Wei" last="Huang">Wei Huang</name>
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<author><name sortKey="Wang, Sheng Qi" sort="Wang, Sheng Qi" uniqKey="Wang S" first="Sheng-Qi" last="Wang">Sheng-Qi Wang</name>
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<author><name sortKey="Ma, Qing Jun" sort="Ma, Qing Jun" uniqKey="Ma Q" first="Qing-Jun" last="Ma">Qing-Jun Ma</name>
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<author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name>
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<series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title>
<idno type="ISSN">1000-3061</idno>
<imprint><date when="2003" type="published">2003</date>
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<term>Chromatography, Ion Exchange</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (isolation & purification)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Viral Structural Proteins (genetics)</term>
<term>Viral Structural Proteins (isolation & purification)</term>
<term>Viral Structural Proteins (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Structural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>SARS Virus</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Structural Proteins</term>
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<term>Nucleocapsid Proteins</term>
<term>SARS Virus</term>
<term>Viral Structural Proteins</term>
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<front><div type="abstract" xml:lang="en">Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.</div>
</front>
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<Month>04</Month>
<Day>29</Day>
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<DateRevised><Year>2005</Year>
<Month>06</Month>
<Day>22</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-3061</ISSN>
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<Issue>4</Issue>
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<Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title>
<ISOAbbreviation>Sheng Wu Gong Cheng Xue Bao</ISOAbbreviation>
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<ArticleTitle>[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].</ArticleTitle>
<Pagination><MedlinePgn>392-6</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.</AbstractText>
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