Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction.
Identifieur interne : 002559 ( PubMed/Corpus ); précédent : 002558; suivant : 002560Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction.
Auteurs : C C Loa ; T L Lin ; C C Wu ; T A Bryan ; T A Hooper ; D L SchraderSource :
- Journal of virological methods [ 0166-0934 ] ; 2006.
English descriptors
- KwdEn :
- Animals, Coronavirus Infections (diagnosis), Coronavirus, Bovine (chemistry), Coronavirus, Bovine (genetics), Coronavirus, Turkey (chemistry), Coronavirus, Turkey (genetics), DNA Primers, Genes, Viral, Infectious bronchitis virus (chemistry), Infectious bronchitis virus (genetics), Membrane Glycoproteins (genetics), Nucleocapsid Proteins (genetics), Polymerase Chain Reaction (methods), RNA, Viral (genetics), Sensitivity and Specificity, Species Specificity, Spike Glycoprotein, Coronavirus, Turkeys, Viral Envelope Proteins (genetics).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Nucleocapsid Proteins, RNA, Viral, Viral Envelope Proteins.
- chemical : DNA Primers, Spike Glycoprotein, Coronavirus.
- chemistry : Coronavirus, Bovine, Coronavirus, Turkey, Infectious bronchitis virus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus, Bovine, Coronavirus, Turkey, Infectious bronchitis virus.
- methods : Polymerase Chain Reaction.
- Animals, Genes, Viral, Sensitivity and Specificity, Species Specificity, Turkeys.
Abstract
The objective of the present study was to develop a multiplex polymerase chain reaction (PCR) method for differential detection of turkey coronavirus (TCoV), infectious bronchitis coronavirus (IBV), and bovine coronavirus (BCoV). Primers were designed from conserved or variable regions of nucleocapsid (N) or spike (S) protein gene among TCoV, IBV, and BCoV and used in the same PCR reaction. Reverse transcription followed by the PCR reaction was used to amplify a portion of N or S gene of the corresponding coronaviruses. The PCR products were detected on agarose gel stained with ethidium bromide. Two PCR products, a 356-bp band corresponding to N gene and a 727-bp band corresponding to S gene, were obtained for TCoV isolates. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene was obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene was obtained for BCoV. There were no PCR products with the same primers for Newcastle disease virus, Marek's disease virus, turkey pox virus, pigeon pox virus, fowl pox virus, reovirus, infectious bursal disease virus, enterovirus, astrovirus, Salmonella enterica, Escherichia coli, and Mycoplasma gallisepticum. Performance of the assay with serially diluted RNA demonstrated that the multiplex PCR could detect 4.8x10(-3) microg of TCoV RNA, 4.6x10(-4) microg of IBV RNA, and 8.0x10(-2) microg of BCoV RNA. These results indicated that the multiplex PCR as established in the present study is a rapid, sensitive, and specific method for differential detection of TCoV, IBV, and BCoV in a single PCR reaction.
DOI: 10.1016/j.jviromet.2005.07.007
PubMed: 16137773
Links to Exploration step
pubmed:16137773Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction.</title><author><name sortKey="Loa, C C" sort="Loa, C C" uniqKey="Loa C" first="C C" last="Loa">C C Loa</name><affiliation><nlm:affiliation>Department of Veterinary Pathobiology and Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907-2065, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lin, T L" sort="Lin, T L" uniqKey="Lin T" first="T L" last="Lin">T L Lin</name></author><author><name sortKey="Wu, C C" sort="Wu, C C" uniqKey="Wu C" first="C C" last="Wu">C C Wu</name></author><author><name sortKey="Bryan, T A" sort="Bryan, T A" uniqKey="Bryan T" first="T A" last="Bryan">T A Bryan</name></author><author><name sortKey="Hooper, T A" sort="Hooper, T A" uniqKey="Hooper T" first="T A" last="Hooper">T A Hooper</name></author><author><name sortKey="Schrader, D L" sort="Schrader, D L" uniqKey="Schrader D" first="D L" last="Schrader">D L Schrader</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16137773</idno><idno type="pmid">16137773</idno><idno type="doi">10.1016/j.jviromet.2005.07.007</idno><idno type="wicri:Area/PubMed/Corpus">002559</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002559</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction.</title><author><name sortKey="Loa, C C" sort="Loa, C C" uniqKey="Loa C" first="C C" last="Loa">C C Loa</name><affiliation><nlm:affiliation>Department of Veterinary Pathobiology and Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907-2065, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lin, T L" sort="Lin, T L" uniqKey="Lin T" first="T L" last="Lin">T L Lin</name></author><author><name sortKey="Wu, C C" sort="Wu, C C" uniqKey="Wu C" first="C C" last="Wu">C C Wu</name></author><author><name sortKey="Bryan, T A" sort="Bryan, T A" uniqKey="Bryan T" first="T A" last="Bryan">T A Bryan</name></author><author><name sortKey="Hooper, T A" sort="Hooper, T A" uniqKey="Hooper T" first="T A" last="Hooper">T A Hooper</name></author><author><name sortKey="Schrader, D L" sort="Schrader, D L" uniqKey="Schrader D" first="D L" last="Schrader">D L Schrader</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus, Bovine (chemistry)</term><term>Coronavirus, Bovine (genetics)</term><term>Coronavirus, Turkey (chemistry)</term><term>Coronavirus, Turkey (genetics)</term><term>DNA Primers</term><term>Genes, Viral</term><term>Infectious bronchitis virus (chemistry)</term><term>Infectious bronchitis virus (genetics)</term><term>Membrane Glycoproteins (genetics)</term><term>Nucleocapsid Proteins (genetics)</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Viral (genetics)</term><term>Sensitivity and Specificity</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term><term>Turkeys</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Coronavirus, Bovine</term><term>Coronavirus, Turkey</term><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus, Bovine</term><term>Coronavirus, Turkey</term><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genes, Viral</term><term>Sensitivity and Specificity</term><term>Species Specificity</term><term>Turkeys</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of the present study was to develop a multiplex polymerase chain reaction (PCR) method for differential detection of turkey coronavirus (TCoV), infectious bronchitis coronavirus (IBV), and bovine coronavirus (BCoV). Primers were designed from conserved or variable regions of nucleocapsid (N) or spike (S) protein gene among TCoV, IBV, and BCoV and used in the same PCR reaction. Reverse transcription followed by the PCR reaction was used to amplify a portion of N or S gene of the corresponding coronaviruses. The PCR products were detected on agarose gel stained with ethidium bromide. Two PCR products, a 356-bp band corresponding to N gene and a 727-bp band corresponding to S gene, were obtained for TCoV isolates. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene was obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene was obtained for BCoV. There were no PCR products with the same primers for Newcastle disease virus, Marek's disease virus, turkey pox virus, pigeon pox virus, fowl pox virus, reovirus, infectious bursal disease virus, enterovirus, astrovirus, Salmonella enterica, Escherichia coli, and Mycoplasma gallisepticum. Performance of the assay with serially diluted RNA demonstrated that the multiplex PCR could detect 4.8x10(-3) microg of TCoV RNA, 4.6x10(-4) microg of IBV RNA, and 8.0x10(-2) microg of BCoV RNA. These results indicated that the multiplex PCR as established in the present study is a rapid, sensitive, and specific method for differential detection of TCoV, IBV, and BCoV in a single PCR reaction.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16137773</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0166-0934</ISSN><JournalIssue CitedMedium="Print"><Volume>131</Volume><Issue>1</Issue><PubDate><Year>2006</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction.</ArticleTitle><Pagination><MedlinePgn>86-91</MedlinePgn></Pagination><Abstract><AbstractText>The objective of the present study was to develop a multiplex polymerase chain reaction (PCR) method for differential detection of turkey coronavirus (TCoV), infectious bronchitis coronavirus (IBV), and bovine coronavirus (BCoV). Primers were designed from conserved or variable regions of nucleocapsid (N) or spike (S) protein gene among TCoV, IBV, and BCoV and used in the same PCR reaction. Reverse transcription followed by the PCR reaction was used to amplify a portion of N or S gene of the corresponding coronaviruses. The PCR products were detected on agarose gel stained with ethidium bromide. Two PCR products, a 356-bp band corresponding to N gene and a 727-bp band corresponding to S gene, were obtained for TCoV isolates. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene was obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene was obtained for BCoV. There were no PCR products with the same primers for Newcastle disease virus, Marek's disease virus, turkey pox virus, pigeon pox virus, fowl pox virus, reovirus, infectious bursal disease virus, enterovirus, astrovirus, Salmonella enterica, Escherichia coli, and Mycoplasma gallisepticum. Performance of the assay with serially diluted RNA demonstrated that the multiplex PCR could detect 4.8x10(-3) microg of TCoV RNA, 4.6x10(-4) microg of IBV RNA, and 8.0x10(-2) microg of BCoV RNA. These results indicated that the multiplex PCR as established in the present study is a rapid, sensitive, and specific method for differential detection of TCoV, IBV, and BCoV in a single PCR reaction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Loa</LastName><ForeName>C C</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Department of Veterinary Pathobiology and Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907-2065, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>T L</ForeName><Initials>TL</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>C C</ForeName><Initials>CC</Initials></Author><Author ValidYN="Y"><LastName>Bryan</LastName><ForeName>T A</ForeName><Initials>TA</Initials></Author><Author ValidYN="Y"><LastName>Hooper</LastName><ForeName>T A</ForeName><Initials>TA</Initials></Author><Author ValidYN="Y"><LastName>Schrader</LastName><ForeName>D L</ForeName><Initials>DL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>08</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName 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