SrasV1, PubMed, Corpus, bibRecord, 002493

A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study.

Identifieur interne : 002493 ( PubMed/Corpus ); précédent : 002492; suivant : 002494

A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study.

Auteurs : Grace T Y. Chung ; Rossa W K. Chiu ; Jo L K. Cheung ; Yongjie Jin ; Stephen S C. Chim ; Paul K S. Chan ; Y M Dennis Lo

Source :

BMC infectious diseases [ 1471-2334 ] ; 2005.

RBID : pubmed:16229749

English descriptors

KwdEn :
- Alleles, Evolution, Molecular, Genes, Viral (genetics), Genotype, Humans, Oligonucleotide Probes (analysis), Oligonucleotide Probes (genetics), Phylogeny, SARS Virus (genetics), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Time Factors.
MESH :
- chemical , analysis : Oligonucleotide Probes.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : Genes, Viral, Oligonucleotide Probes, SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Alleles, Evolution, Molecular, Genotype, Humans, Phylogeny, Time Factors.

Abstract

The Severe Acute Respiratory Syndrome (SARS) was a newly emerged infectious disease which caused a global epidemic in 2002-2003. Sequence analysis of SARS-coronavirus isolates revealed that specific genotypes predominated at different periods of the epidemic. This information can be used as a footprint for tracing the epidemiology of infections and monitor viral evolution. However, direct sequencing analysis of a large number of clinical samples is cumbersome and time consuming. We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination.

DOI: 10.1186/1471-2334-5-87
PubMed: 16229749

Links to Exploration step

pubmed:16229749

Le document en format XML

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<author><name sortKey="Chung, Grace T Y" sort="Chung, Grace T Y" uniqKey="Chung G" first="Grace T Y" last="Chung">Grace T Y. Chung</name>
<affiliation><nlm:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong. gracehung@cuhk.edu.hk</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Chiu, Rossa W K" sort="Chiu, Rossa W K" uniqKey="Chiu R" first="Rossa W K" last="Chiu">Rossa W K. Chiu</name>
</author>
<author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name>
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<author><name sortKey="Jin, Yongjie" sort="Jin, Yongjie" uniqKey="Jin Y" first="Yongjie" last="Jin">Yongjie Jin</name>
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<author><name sortKey="Chim, Stephen S C" sort="Chim, Stephen S C" uniqKey="Chim S" first="Stephen S C" last="Chim">Stephen S C. Chim</name>
</author>
<author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name>
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<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name>
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<author><name sortKey="Chung, Grace T Y" sort="Chung, Grace T Y" uniqKey="Chung G" first="Grace T Y" last="Chung">Grace T Y. Chung</name>
<affiliation><nlm:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong. gracehung@cuhk.edu.hk</nlm:affiliation>
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<author><name sortKey="Chiu, Rossa W K" sort="Chiu, Rossa W K" uniqKey="Chiu R" first="Rossa W K" last="Chiu">Rossa W K. Chiu</name>
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<author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name>
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<author><name sortKey="Jin, Yongjie" sort="Jin, Yongjie" uniqKey="Jin Y" first="Yongjie" last="Jin">Yongjie Jin</name>
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<author><name sortKey="Chim, Stephen S C" sort="Chim, Stephen S C" uniqKey="Chim S" first="Stephen S C" last="Chim">Stephen S C. Chim</name>
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<author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name>
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<author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name>
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<series><title level="j">BMC infectious diseases</title>
<idno type="eISSN">1471-2334</idno>
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<term>Evolution, Molecular</term>
<term>Genes, Viral (genetics)</term>
<term>Genotype</term>
<term>Humans</term>
<term>Oligonucleotide Probes (analysis)</term>
<term>Oligonucleotide Probes (genetics)</term>
<term>Phylogeny</term>
<term>SARS Virus (genetics)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligonucleotide Probes</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Viral</term>
<term>Oligonucleotide Probes</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Alleles</term>
<term>Evolution, Molecular</term>
<term>Genotype</term>
<term>Humans</term>
<term>Phylogeny</term>
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<front><div type="abstract" xml:lang="en">The Severe Acute Respiratory Syndrome (SARS) was a newly emerged infectious disease which caused a global epidemic in 2002-2003. Sequence analysis of SARS-coronavirus isolates revealed that specific genotypes predominated at different periods of the epidemic. This information can be used as a footprint for tracing the epidemiology of infections and monitor viral evolution. However, direct sequencing analysis of a large number of clinical samples is cumbersome and time consuming. We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination.</div>
</front>
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<DateCompleted><Year>2006</Year>
<Month>05</Month>
<Day>05</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2334</ISSN>
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<PubDate><Year>2005</Year>
<Month>Oct</Month>
<Day>18</Day>
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<Title>BMC infectious diseases</Title>
<ISOAbbreviation>BMC Infect. Dis.</ISOAbbreviation>
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<ArticleTitle>A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study.</ArticleTitle>
<Pagination><MedlinePgn>87</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The Severe Acute Respiratory Syndrome (SARS) was a newly emerged infectious disease which caused a global epidemic in 2002-2003. Sequence analysis of SARS-coronavirus isolates revealed that specific genotypes predominated at different periods of the epidemic. This information can be used as a footprint for tracing the epidemiology of infections and monitor viral evolution. However, direct sequencing analysis of a large number of clinical samples is cumbersome and time consuming. We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Thirty SARS patients were recruited. Allelic discrimination assays were developed based on the use of fluorogenic oligonucleotide probes (TaqMan). Genotyping of the SARS-coronavirus isolates obtained from these patients were carried out by the allelic discrimination assays and confirmed by direct sequencing.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Genotyping based on the allelic discrimination assays were fully concordant with direct sequencing. All of the 30 SARS-coronavirus genotypes studied were characteristic of genotypes previously documented to be associated with the latter part of the epidemic. Seven of the isolates contained a previously reported major deletion but in patients not epidemiologically related to the previously studied cohort.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">We have developed a simple and accurate method for the characterization and screening of SARS-coronavirus genotypes. It is a promising tool for the study of epidemiological relationships between documented cases during an outbreak.</AbstractText>
</Abstract>
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<ForeName>Grace T Y</ForeName>
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<AffiliationInfo><Affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong. gracehung@cuhk.edu.hk</Affiliation>
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<Language>eng</Language>
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A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study.

A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki