SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity.
Identifieur interne : 002307 ( PubMed/Corpus ); précédent : 002306; suivant : 002308SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity.
Auteurs : Mi-Fang Liang ; Run-Lei Du ; Jing-Zhi Liu ; Chuan Li ; Quan-Fu Zhang ; Lu-Lu Han ; Jian-Shi Yu ; Shu-Min Duan ; Xiao-Fang Wang ; Kong-Xing Wu ; Zhao-Hui Xiong ; Qi Jin ; De-Xin LiSource :
- Biomedical and environmental sciences : BES [ 0895-3988 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (immunology), Chlorocebus aethiops, Humans, Membrane Glycoproteins (immunology), Neutralization Tests, Peptide Library, Protein Binding, Protein Engineering, Recombinant Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (immunology), Viral Matrix Proteins (immunology).
- MESH :
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Humans, Neutralization Tests, Peptide Library, Protein Binding, Protein Engineering, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
To develop a specific SARS virus-targeted antibody preparation for emergent prophylaxis and treatment of SARS virus infection.
PubMed: 16544518
Links to Exploration step
pubmed:16544518Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity.</title>
<author><name sortKey="Liang, Mi Fang" sort="Liang, Mi Fang" uniqKey="Liang M" first="Mi-Fang" last="Liang">Mi-Fang Liang</name>
<affiliation><nlm:affiliation>State Key Laboratory for Infectious Disease Control and Prevention, Institute for Viral Disease Control and Prevention, Beijing 100052, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Du, Run Lei" sort="Du, Run Lei" uniqKey="Du R" first="Run-Lei" last="Du">Run-Lei Du</name>
</author>
<author><name sortKey="Liu, Jing Zhi" sort="Liu, Jing Zhi" uniqKey="Liu J" first="Jing-Zhi" last="Liu">Jing-Zhi Liu</name>
</author>
<author><name sortKey="Li, Chuan" sort="Li, Chuan" uniqKey="Li C" first="Chuan" last="Li">Chuan Li</name>
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<author><name sortKey="Zhang, Quan Fu" sort="Zhang, Quan Fu" uniqKey="Zhang Q" first="Quan-Fu" last="Zhang">Quan-Fu Zhang</name>
</author>
<author><name sortKey="Han, Lu Lu" sort="Han, Lu Lu" uniqKey="Han L" first="Lu-Lu" last="Han">Lu-Lu Han</name>
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<author><name sortKey="Yu, Jian Shi" sort="Yu, Jian Shi" uniqKey="Yu J" first="Jian-Shi" last="Yu">Jian-Shi Yu</name>
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<author><name sortKey="Duan, Shu Min" sort="Duan, Shu Min" uniqKey="Duan S" first="Shu-Min" last="Duan">Shu-Min Duan</name>
</author>
<author><name sortKey="Wang, Xiao Fang" sort="Wang, Xiao Fang" uniqKey="Wang X" first="Xiao-Fang" last="Wang">Xiao-Fang Wang</name>
</author>
<author><name sortKey="Wu, Kong Xing" sort="Wu, Kong Xing" uniqKey="Wu K" first="Kong-Xing" last="Wu">Kong-Xing Wu</name>
</author>
<author><name sortKey="Xiong, Zhao Hui" sort="Xiong, Zhao Hui" uniqKey="Xiong Z" first="Zhao-Hui" last="Xiong">Zhao-Hui Xiong</name>
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<author><name sortKey="Jin, Qi" sort="Jin, Qi" uniqKey="Jin Q" first="Qi" last="Jin">Qi Jin</name>
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<author><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity.</title>
<author><name sortKey="Liang, Mi Fang" sort="Liang, Mi Fang" uniqKey="Liang M" first="Mi-Fang" last="Liang">Mi-Fang Liang</name>
<affiliation><nlm:affiliation>State Key Laboratory for Infectious Disease Control and Prevention, Institute for Viral Disease Control and Prevention, Beijing 100052, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Du, Run Lei" sort="Du, Run Lei" uniqKey="Du R" first="Run-Lei" last="Du">Run-Lei Du</name>
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<author><name sortKey="Liu, Jing Zhi" sort="Liu, Jing Zhi" uniqKey="Liu J" first="Jing-Zhi" last="Liu">Jing-Zhi Liu</name>
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<author><name sortKey="Li, Chuan" sort="Li, Chuan" uniqKey="Li C" first="Chuan" last="Li">Chuan Li</name>
</author>
<author><name sortKey="Zhang, Quan Fu" sort="Zhang, Quan Fu" uniqKey="Zhang Q" first="Quan-Fu" last="Zhang">Quan-Fu Zhang</name>
</author>
<author><name sortKey="Han, Lu Lu" sort="Han, Lu Lu" uniqKey="Han L" first="Lu-Lu" last="Han">Lu-Lu Han</name>
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<author><name sortKey="Yu, Jian Shi" sort="Yu, Jian Shi" uniqKey="Yu J" first="Jian-Shi" last="Yu">Jian-Shi Yu</name>
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<author><name sortKey="Duan, Shu Min" sort="Duan, Shu Min" uniqKey="Duan S" first="Shu-Min" last="Duan">Shu-Min Duan</name>
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<author><name sortKey="Wang, Xiao Fang" sort="Wang, Xiao Fang" uniqKey="Wang X" first="Xiao-Fang" last="Wang">Xiao-Fang Wang</name>
</author>
<author><name sortKey="Wu, Kong Xing" sort="Wu, Kong Xing" uniqKey="Wu K" first="Kong-Xing" last="Wu">Kong-Xing Wu</name>
</author>
<author><name sortKey="Xiong, Zhao Hui" sort="Xiong, Zhao Hui" uniqKey="Xiong Z" first="Zhao-Hui" last="Xiong">Zhao-Hui Xiong</name>
</author>
<author><name sortKey="Jin, Qi" sort="Jin, Qi" uniqKey="Jin Q" first="Qi" last="Jin">Qi Jin</name>
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<author><name sortKey="Li, De Xin" sort="Li, De Xin" uniqKey="Li D" first="De-Xin" last="Li">De-Xin Li</name>
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<series><title level="j">Biomedical and environmental sciences : BES</title>
<idno type="ISSN">0895-3988</idno>
<imprint><date when="2005" type="published">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Viral (immunology)</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Neutralization Tests</term>
<term>Peptide Library</term>
<term>Protein Binding</term>
<term>Protein Engineering</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Matrix Proteins (immunology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Neutralization Tests</term>
<term>Peptide Library</term>
<term>Protein Binding</term>
<term>Protein Engineering</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
</keywords>
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<front><div type="abstract" xml:lang="en">To develop a specific SARS virus-targeted antibody preparation for emergent prophylaxis and treatment of SARS virus infection.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16544518</PMID>
<DateCompleted><Year>2006</Year>
<Month>05</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0895-3988</ISSN>
<JournalIssue CitedMedium="Print"><Volume>18</Volume>
<Issue>6</Issue>
<PubDate><Year>2005</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Biomedical and environmental sciences : BES</Title>
<ISOAbbreviation>Biomed. Environ. Sci.</ISOAbbreviation>
</Journal>
<ArticleTitle>SARS patients-derived human recombinant antibodies to S and M proteins efficiently neutralize SARS-coronavirus infectivity.</ArticleTitle>
<Pagination><MedlinePgn>363-74</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop a specific SARS virus-targeted antibody preparation for emergent prophylaxis and treatment of SARS virus infection.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">By using phage display technology, we constructed a naive antibody library from convalescent SARS patient lymphocytes. To obtain the neutralizing antibody to SARS virus surface proteins, the library panning procedure was performed on purified SARS virions and the specific Fab antibody clones were enriched by four rounds of repeated panning procedure and screened by highthroughput selection. The selected Fab antibodies expressed in the periplasma of E. coli were soluble and further purified and tested for their binding properties and antiviral function to SARS virus. The functional Fab antibodies were converted to full human IgG antibodies with recombinant baculovirus/insect cell systems and their neutralizing activities were further determined.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">After four rounds of the panning, a number of SARS-CoV virus-targeted human recombinant Fab antibodies were isolated from the SARS patient antibody library. Most of these were identified to recognize both natural and recombinant SARS spike (S) proteins, two Fab antibodies were specific for the virus membrane (M) protein, only one bound to SARS-CoV nucleocapsid protein. The SARS-CoV S and M protein-targeted Fab or IgG antibodies showed significant neutralizing activities in cytopathic effect (CPE) inhibition neutralization test, these antibodies were able to completely neutralize the SARS virus and protect the Vero cells from CPE after virus infection. However, the N protein-targeted Fab or IgG antibodies failed to neutralize the virus. In addition, the SARS N protein-targeted human Fab antibody reacted with the denatured N proteins, whereas none of the S and M protein specific neutralizing antibodies did. These results suggested that the S and M protein-specific neutralizing antibodies could recognize conformational epitopes which might be involved in the binding of virions to cellular receptors and the fusion activity of the virus.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The SARS-CoV spike protein and membrane proteins are able to elicite efficient neutralizing antibodies in SARS patients. The neutralizing antibodies we generated in this study may be more promising candidates for prophylaxis and treatment of SARS infection.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liang</LastName>
<ForeName>Mi-Fang</ForeName>
<Initials>MF</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory for Infectious Disease Control and Prevention, Institute for Viral Disease Control and Prevention, Beijing 100052, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Du</LastName>
<ForeName>Run-Lei</ForeName>
<Initials>RL</Initials>
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<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Jing-Zhi</ForeName>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Chuan</ForeName>
<Initials>C</Initials>
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<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Quan-Fu</ForeName>
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<Author ValidYN="Y"><LastName>Han</LastName>
<ForeName>Lu-Lu</ForeName>
<Initials>LL</Initials>
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<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Jian-Shi</ForeName>
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<Author ValidYN="Y"><LastName>Duan</LastName>
<ForeName>Shu-Min</ForeName>
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<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Xiao-Fang</ForeName>
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<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Kong-Xing</ForeName>
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<Author ValidYN="Y"><LastName>Xiong</LastName>
<ForeName>Zhao-Hui</ForeName>
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<Author ValidYN="Y"><LastName>Jin</LastName>
<ForeName>Qi</ForeName>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>De-Xin</ForeName>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Biomed Environ Sci</MedlineTA>
<NlmUniqueID>8909524</NlmUniqueID>
<ISSNLinking>0895-3988</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019151">Peptide Library</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
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</Chemical>
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</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>108502-71-2</RegistryNumber>
<NameOfSubstance UI="C067997">M protein, Coronavirus</NameOfSubstance>
</Chemical>
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<MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009500" MajorTopicYN="N">Neutralization Tests</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019151" MajorTopicYN="N">Peptide Library</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
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<MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName>
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<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014763" MajorTopicYN="N">Viral Matrix Proteins</DescriptorName>
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