SrasV1, PubMed, Corpus, bibRecord, 001C63

[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].

Identifieur interne : 001C63 ( PubMed/Corpus ); précédent : 001C62; suivant : 001C64

[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].

Auteurs : Jian-Qiang Kong ; Wei Wang ; Guan-Hua Du ; Ping Zhu ; Ke-Di Cheng

Source :

Yao xue xue bao = Acta pharmaceutica Sinica [ 0513-4870 ] ; 2007.

RBID : pubmed:18050746

English descriptors

KwdEn :
- Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression Regulation, Viral, Genes, Viral, Genetic Vectors, Genome, Viral, Open Reading Frames, Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (genetics), Temperature, Time, Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Viral Proteins (genetics), Viral Proteins (metabolism).
MESH :
- chemical , genetics : Recombinant Proteins, Viral Matrix Proteins, Viral Proteins.
- genetics : Escherichia coli, SARS Virus.
- metabolism : Escherichia coli, Recombinant Proteins, Viral Matrix Proteins, Viral Proteins.
- Gene Expression Regulation, Viral, Genes, Viral, Genetic Vectors, Genome, Viral, Open Reading Frames, Temperature, Time.

Abstract

According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows: X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.

PubMed: 18050746

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pubmed:18050746

Le document en format XML

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<author><name sortKey="Kong, Jian Qiang" sort="Kong, Jian Qiang" uniqKey="Kong J" first="Jian-Qiang" last="Kong">Jian-Qiang Kong</name>
<affiliation><nlm:affiliation>Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
</author>
<author><name sortKey="Du, Guan Hua" sort="Du, Guan Hua" uniqKey="Du G" first="Guan-Hua" last="Du">Guan-Hua Du</name>
</author>
<author><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name>
</author>
<author><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].</title>
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<affiliation><nlm:affiliation>Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.</nlm:affiliation>
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<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
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<author><name sortKey="Du, Guan Hua" sort="Du, Guan Hua" uniqKey="Du G" first="Guan-Hua" last="Du">Guan-Hua Du</name>
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<author><name sortKey="Zhu, Ping" sort="Zhu, Ping" uniqKey="Zhu P" first="Ping" last="Zhu">Ping Zhu</name>
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<author><name sortKey="Cheng, Ke Di" sort="Cheng, Ke Di" uniqKey="Cheng K" first="Ke-Di" last="Cheng">Ke-Di Cheng</name>
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<series><title level="j">Yao xue xue bao = Acta pharmaceutica Sinica</title>
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<term>Genes, Viral</term>
<term>Genetic Vectors</term>
<term>Genome, Viral</term>
<term>Open Reading Frames</term>
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<term>Recombinant Proteins (metabolism)</term>
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<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Viral</term>
<term>Genes, Viral</term>
<term>Genetic Vectors</term>
<term>Genome, Viral</term>
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<front><div type="abstract" xml:lang="en">According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows: X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.</div>
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<Month>Sep</Month>
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<Title>Yao xue xue bao = Acta pharmaceutica Sinica</Title>
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<ArticleTitle>[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].</ArticleTitle>
<Pagination><MedlinePgn>1000-6</MedlinePgn>
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<Abstract><AbstractText>According to previous studies of SARS-CoV (Severe acute respiratory syndrome coronavirus), a variety of novel accessory genes have been identified in SARS-CoV genome, which were interspersed the structural genes of SARS-CoV and considered to be unique to the SARS-CoV genome. The predicted unknown proteins (PUPs) encoded by the accessory genes might play important roles in the SARS-CoV infection. Three of those genes, called X4, X5 and ORF10, were synthesized and introduced into E. coli to induce expression. SDS-PAGE and Western blotting revealed that the three genes have been expressed in E. coli. The induction of SARS PUPs genes expression in different temperatures, induction times, IPTG concentrations and A values of E. coli cells were performed. The optimal induction condition of SARS-CoV PUPs genes was characterized according to the orthorgonal analysis. The ratio of recombinant proteins of PUPs to total proteins is as follows: X4, 20%; X5, 27.8%; ORF10, 68.5% under the optimum conditions.</AbstractText>
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[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].

[Optimization of expression condition of SARS-CoV PUPs genes in E. coli].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki