[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].
Identifieur interne : 001939 ( PubMed/Corpus ); précédent : 001938; suivant : 001940[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].
Auteurs : Yun Liu ; Ai-Hua Liu ; Peng Deng ; Xiang-Ling Wu ; Tao Li ; Ya-Wei Liu ; Jia Xu ; Yong JiangSource :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University [ 1673-4254 ] ; 2009.
English descriptors
- KwdEn :
- Escherichia coli (genetics), Escherichia coli (metabolism), Green Fluorescent Proteins (biosynthesis), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), HeLa Cells, Humans, Membrane Fusion (drug effects), Membrane Fusion Proteins (biosynthesis), Membrane Fusion Proteins (isolation & purification), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (isolation & purification), SARS Virus (genetics), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics).
- MESH :
- chemical , biosynthesis : Green Fluorescent Proteins, Membrane Fusion Proteins, Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- drug effects : Membrane Fusion.
- genetics : Escherichia coli, Green Fluorescent Proteins, Membrane Glycoproteins, Recombinant Fusion Proteins, SARS Virus, Viral Envelope Proteins.
- chemical , isolation & purification : Membrane Fusion Proteins, Recombinant Fusion Proteins.
- metabolism : Escherichia coli, Green Fluorescent Proteins.
- HeLa Cells, Humans, Spike Glycoprotein, Coronavirus.
Abstract
To construct the expression plasmid of S2 extracellular domain (S2ED) of SARS-coronavirus (SARS- Cov) spike protein (S protein) and enhanced green fluorescent protein (EGFP) to obtain the fusion protein expressed in prokaryotic cells.
PubMed: 19304506
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pubmed:19304506Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].</title><author><name sortKey="Liu, Yun" sort="Liu, Yun" uniqKey="Liu Y" first="Yun" last="Liu">Yun Liu</name><affiliation><nlm:affiliation>Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou, China. sysliuyun@126.com</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ai Hua" sort="Liu, Ai Hua" uniqKey="Liu A" first="Ai-Hua" last="Liu">Ai-Hua Liu</name></author><author><name sortKey="Deng, Peng" sort="Deng, Peng" uniqKey="Deng P" first="Peng" last="Deng">Peng Deng</name></author><author><name sortKey="Wu, Xiang Ling" sort="Wu, Xiang Ling" uniqKey="Wu X" first="Xiang-Ling" last="Wu">Xiang-Ling Wu</name></author><author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name></author><author><name sortKey="Liu, Ya Wei" sort="Liu, Ya Wei" uniqKey="Liu Y" first="Ya-Wei" last="Liu">Ya-Wei Liu</name></author><author><name sortKey="Xu, Jia" sort="Xu, Jia" uniqKey="Xu J" first="Jia" last="Xu">Jia Xu</name></author><author><name sortKey="Jiang, Yong" sort="Jiang, Yong" uniqKey="Jiang Y" first="Yong" last="Jiang">Yong Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19304506</idno><idno type="pmid">19304506</idno><idno type="wicri:Area/PubMed/Corpus">001939</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001939</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].</title><author><name sortKey="Liu, Yun" sort="Liu, Yun" uniqKey="Liu Y" first="Yun" last="Liu">Yun Liu</name><affiliation><nlm:affiliation>Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou, China. sysliuyun@126.com</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ai Hua" sort="Liu, Ai Hua" uniqKey="Liu A" first="Ai-Hua" last="Liu">Ai-Hua Liu</name></author><author><name sortKey="Deng, Peng" sort="Deng, Peng" uniqKey="Deng P" first="Peng" last="Deng">Peng Deng</name></author><author><name sortKey="Wu, Xiang Ling" sort="Wu, Xiang Ling" uniqKey="Wu X" first="Xiang-Ling" last="Wu">Xiang-Ling Wu</name></author><author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name></author><author><name sortKey="Liu, Ya Wei" sort="Liu, Ya Wei" uniqKey="Liu Y" first="Ya-Wei" last="Liu">Ya-Wei Liu</name></author><author><name sortKey="Xu, Jia" sort="Xu, Jia" uniqKey="Xu J" first="Jia" last="Xu">Jia Xu</name></author><author><name sortKey="Jiang, Yong" sort="Jiang, Yong" uniqKey="Jiang Y" first="Yong" last="Jiang">Yong Jiang</name></author></analytic><series><title level="j">Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><idno type="ISSN">1673-4254</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Green Fluorescent Proteins (biosynthesis)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>HeLa Cells</term><term>Humans</term><term>Membrane Fusion (drug effects)</term><term>Membrane Fusion Proteins (biosynthesis)</term><term>Membrane Fusion Proteins (isolation & purification)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (isolation & purification)</term><term>SARS Virus (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Membrane Fusion Proteins</term><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Membrane Fusion</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Green Fluorescent Proteins</term><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>SARS Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Membrane Fusion Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>HeLa Cells</term><term>Humans</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To construct the expression plasmid of S2 extracellular domain (S2ED) of SARS-coronavirus (SARS- Cov) spike protein (S protein) and enhanced green fluorescent protein (EGFP) to obtain the fusion protein expressed in prokaryotic cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19304506</PMID><DateCompleted><Year>2010</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1673-4254</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</Title><ISOAbbreviation>Nan Fang Yi Ke Da Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].</ArticleTitle><Pagination><MedlinePgn>381-6</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To construct the expression plasmid of S2 extracellular domain (S2ED) of SARS-coronavirus (SARS- Cov) spike protein (S protein) and enhanced green fluorescent protein (EGFP) to obtain the fusion protein expressed in prokaryotic cells.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">S2ED based on bioinformatics prediction and EGFP sequence were amplified by PCR and inserted into pET-14b plasmid. The recombinant protein His-S2ED-EGFP was expressed in E. coli by IPTG induction. After purification by Ni-NTA agarose beads, the soluble fractions of the fusion protein were collected and identified by SDS-PAGE and Western blotting. The fusion of S2ED with Hela cell membranes was observed with fluorescent microscope.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The pET-14b-S2ED-EGFP plasmid was correctly constructed and highly expressed in BL21 (DE3). When incubated with Hela cells, the purified protein could not internalize through membrane fusion.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The expression plasmid containing S2ED of SARS-Cov S protein and EGFP sequence is constructed successfully. Although the recombinant protein obtained has not shown the expected fusion effect with Hela cell membrane, this work may enrich the understanding of the process of membrane fusion mediated by S2 protein and lay the foundation for future study of targeting cell transport system based on cell-specific binding peptide.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou, China. sysliuyun@126.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ai-Hua</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Peng</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiang-Ling</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Tao</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ya-Wei</ForeName><Initials>YW</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jia</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Yong</ForeName><Initials>Y</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Nan Fang Yi Ke Da Xue Xue Bao</MedlineTA><NlmUniqueID>101266132</NlmUniqueID><ISSNLinking>1673-4254</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050576">Membrane Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C485184">enhanced green fluorescent protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006367" MajorTopicYN="N">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008561" MajorTopicYN="N">Membrane Fusion</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050576" MajorTopicYN="N">Membrane Fusion Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19304506</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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