Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification.
Identifieur interne : 001309 ( PubMed/Corpus ); précédent : 001308; suivant : 001310Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification.
Auteurs : Christine M. Malboeuf ; Xiao Yang ; Patrick Charlebois ; James Qu ; Aaron M. Berlin ; Monica Casali ; Kendra N. Pesko ; Christian L. Boutwell ; John P. Devincenzo ; Gregory D. Ebel ; Todd M. Allen ; Michael C. Zody ; Matthew R. Henn ; Joshua Z. LevinSource :
- Nucleic acids research [ 1362-4962 ] ; 2013.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA, Viral.
- genetics : HIV, Respiratory Syncytial Viruses, West Nile virus.
- Base Sequence, Genome, Viral, Humans, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, RNA.
Abstract
RNA viruses are the causative agents for AIDS, influenza, SARS, and other serious health threats. Development of rapid and broadly applicable methods for complete viral genome sequencing is highly desirable to fully understand all aspects of these infectious agents as well as for surveillance of viral pandemic threats and emerging pathogens. However, traditional viral detection methods rely on prior sequence or antigen knowledge. In this study, we describe sequence-independent amplification for samples containing ultra-low amounts of viral RNA coupled with Illumina sequencing and de novo assembly optimized for viral genomes. With 5 million reads, we capture 96 to 100% of the viral protein coding region of HIV, respiratory syncytial and West Nile viral samples from as little as 100 copies of viral RNA. The methods presented here are scalable to large numbers of samples and capable of generating full or near full length viral genomes from clone and clinical samples with low amounts of viral RNA, without prior sequence information and in the presence of substantial host contamination.
DOI: 10.1093/nar/gks794
PubMed: 22962364
Links to Exploration step
pubmed:22962364Le document en format XML
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<front><div type="abstract" xml:lang="en">RNA viruses are the causative agents for AIDS, influenza, SARS, and other serious health threats. Development of rapid and broadly applicable methods for complete viral genome sequencing is highly desirable to fully understand all aspects of these infectious agents as well as for surveillance of viral pandemic threats and emerging pathogens. However, traditional viral detection methods rely on prior sequence or antigen knowledge. In this study, we describe sequence-independent amplification for samples containing ultra-low amounts of viral RNA coupled with Illumina sequencing and de novo assembly optimized for viral genomes. With 5 million reads, we capture 96 to 100% of the viral protein coding region of HIV, respiratory syncytial and West Nile viral samples from as little as 100 copies of viral RNA. The methods presented here are scalable to large numbers of samples and capable of generating full or near full length viral genomes from clone and clinical samples with low amounts of viral RNA, without prior sequence information and in the presence of substantial host contamination.</div>
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<ReferenceList><Reference><Citation>J Virol. 2008 Sep;82(17):8422-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18562530</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechniques. 2008 Jul;45(1):81-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18611170</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 May;83(9):4642-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19211756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2009;4(5):e5683</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19479085</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Aug 6;460(7256):711-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19661910</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2010 Feb;20(2):265-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20019144</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2010 May;6(5):e1000896</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20463816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Jun;84(12):6218-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20375170</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(5):e10588</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20485673</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2010 Sep;7(9):709-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20711195</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(8):e12303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20808830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Nov;84(22):12087-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20844037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(10):e13564</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21042592</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Comput Biol. 2010;6(12):e1001022</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21187908</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Mar;85(5):2266-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21159860</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2010;11(12):R119</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21143862</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(5):e19838</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21603639</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Prev Res (Phila). 2011 Jun;4(6):803-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21636547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2011 Sep;49(9):3268-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21715589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2011 Sep;21(9):1552-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21690186</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Negl Trop Dis. 2011 Sep;5(9):e1309</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21909448</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2011 Oct;39(18):e120</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21737426</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2011 Nov;77(22):8071-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21948828</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2012 Mar;50(3):857-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22205816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Apr;86(7):3952-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22278255</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2012 May 25;427(1):10-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22365325</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2012;8(3):e1002529</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22412369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Aug;86(16):8546-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22647702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Comput Biol. 2000 Feb-Apr;7(1-2):203-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10890397</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 Jun;76(12):5847-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12021317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2003 Aug;41(8):3661-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12904372</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004;32(5):1792-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15034147</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2004 Jul 10;20(10):1495-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15231541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1986 Aug;59(2):284-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3016298</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1997 Feb 1;25(3):654-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9016609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1998 Jul 15;26(14):3433-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9649630</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pediatr Res. 2004 Dec;56(6):914-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15470202</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2005 Jan;43(1):406-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15635002</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2005 May;43(5):2356-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15872266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2005 Oct;51(10):1973-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16123149</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2007;35(13):e91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17576693</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2007 Aug;17(8):1195-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17600086</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunity. 2007 Sep;27(3):406-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17892849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2008;9:5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18179705</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Apr;82(8):3952-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18256145</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Diagn. 2008 May;10(3):185-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18403608</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trans R Soc Trop Med Hyg. 2008 Aug;102(8):817-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18466940</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2009 Mar 1;199(5):693-701</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19210162</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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