Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.
Identifieur interne : 001308 ( PubMed/Corpus ); précédent : 001307; suivant : 001309Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.
Auteurs : Jun Huang ; Yingnan Cao ; Xianzhang Bu ; Changyou WuSource :
- BMC immunology [ 1471-2172 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, Computational Biology, Enzyme-Linked Immunospot Assay, Epitope Mapping (methods), Epitopes, T-Lymphocyte (immunology), Epitopes, T-Lymphocyte (metabolism), Female, Interferon-gamma (metabolism), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Peptide Fragments (immunology), Peptide Fragments (metabolism), Protein Binding, SARS Virus (immunology), Sequence Analysis, Protein, Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , immunology : Epitopes, T-Lymphocyte, Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- chemical , metabolism : Epitopes, T-Lymphocyte, Interferon-gamma, Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- methods : Epitope Mapping.
- Animals, Cells, Cultured, Computational Biology, Enzyme-Linked Immunospot Assay, Female, Mice, Mice, Inbred BALB C, Protein Binding, Sequence Analysis, Protein, Spike Glycoprotein, Coronavirus.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by the novel coronavirus SARS-CoV. The T cell epitopes of the SARS CoV spike protein are well known, but no systematic evaluation of the functional and structural roles of each residue has been reported for these antigenic epitopes. Analysis of the functional importance of side-chains by mutational study may exaggerate the effect by imposing a structural disturbance or an unusual steric, electrostatic or hydrophobic interaction.
DOI: 10.1186/1471-2172-13-50
PubMed: 22963340
Links to Exploration step
pubmed:22963340Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.</title><author><name sortKey="Huang, Jun" sort="Huang, Jun" uniqKey="Huang J" first="Jun" last="Huang">Jun Huang</name><affiliation><nlm:affiliation>Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Yingnan" sort="Cao, Yingnan" uniqKey="Cao Y" first="Yingnan" last="Cao">Yingnan Cao</name></author><author><name sortKey="Bu, Xianzhang" sort="Bu, Xianzhang" uniqKey="Bu X" first="Xianzhang" last="Bu">Xianzhang Bu</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22963340</idno><idno type="pmid">22963340</idno><idno type="doi">10.1186/1471-2172-13-50</idno><idno type="wicri:Area/PubMed/Corpus">001308</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001308</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.</title><author><name sortKey="Huang, Jun" sort="Huang, Jun" uniqKey="Huang J" first="Jun" last="Huang">Jun Huang</name><affiliation><nlm:affiliation>Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cao, Yingnan" sort="Cao, Yingnan" uniqKey="Cao Y" first="Yingnan" last="Cao">Yingnan Cao</name></author><author><name sortKey="Bu, Xianzhang" sort="Bu, Xianzhang" uniqKey="Bu X" first="Xianzhang" last="Bu">Xianzhang Bu</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></analytic><series><title level="j">BMC immunology</title><idno type="eISSN">1471-2172</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Computational Biology</term><term>Enzyme-Linked Immunospot Assay</term><term>Epitope Mapping (methods)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Epitopes, T-Lymphocyte (metabolism)</term><term>Female</term><term>Interferon-gamma (metabolism)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Peptide Fragments (immunology)</term><term>Peptide Fragments (metabolism)</term><term>Protein Binding</term><term>SARS Virus (immunology)</term><term>Sequence Analysis, Protein</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Epitopes, T-Lymphocyte</term><term>Interferon-gamma</term><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Computational Biology</term><term>Enzyme-Linked Immunospot Assay</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Protein Binding</term><term>Sequence Analysis, Protein</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by the novel coronavirus SARS-CoV. The T cell epitopes of the SARS CoV spike protein are well known, but no systematic evaluation of the functional and structural roles of each residue has been reported for these antigenic epitopes. Analysis of the functional importance of side-chains by mutational study may exaggerate the effect by imposing a structural disturbance or an unusual steric, electrostatic or hydrophobic interaction.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22963340</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2172</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><PubDate><Year>2012</Year><Month>Sep</Month><Day>10</Day></PubDate></JournalIssue><Title>BMC immunology</Title><ISOAbbreviation>BMC Immunol.</ISOAbbreviation></Journal><ArticleTitle>Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.</ArticleTitle><Pagination><MedlinePgn>50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2172-13-50</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by the novel coronavirus SARS-CoV. The T cell epitopes of the SARS CoV spike protein are well known, but no systematic evaluation of the functional and structural roles of each residue has been reported for these antigenic epitopes. Analysis of the functional importance of side-chains by mutational study may exaggerate the effect by imposing a structural disturbance or an unusual steric, electrostatic or hydrophobic interaction.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We demonstrated that N50 could induce significant IFN-gamma response from SARS-CoV S DNA immunized mice splenocytes by the means of ELISA, ELISPOT and FACS. Moreover, S366-374 was predicted to be an optimal epitope by bioinformatics tools: ANN, SMM, ARB and BIMAS, and confirmed by IFN-gamma response induced by a series of S358-374-derived peptides. Furthermore, each of S366-374 was replaced by alanine (A), lysine (K) or aspartic acid (D), respectively. ANN was used to estimate the binding affinity of single S366-374 mutants to H-2 Kd. Y367 and L374 were predicated to possess the most important role in peptide binding. Additionally, these one residue mutated peptides were synthesized, and IFN-gamma production induced by G368, V369, A371, T372 and K373 mutated S366-374 were decreased obviously.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We demonstrated that S366-374 is an optimal H-2 Kd CTL epitope in the SARS CoV S protein. Moreover, Y367, S370, and L374 are anchors in the epitope, while C366, G368, V369, A371, T372, and K373 may directly interact with TCR on the surface of CD8-T cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Yingnan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Bu</LastName><ForeName>Xianzhang</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Changyou</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Immunol</MedlineTA><NlmUniqueID>100966980</NlmUniqueID><ISSNLinking>1471-2172</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018984">Epitopes, T-Lymphocyte</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical><Chemical><RegistryNumber>82115-62-6</RegistryNumber><NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058501" MajorTopicYN="N">Enzyme-Linked Immunospot Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018604" MajorTopicYN="N">Epitope Mapping</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018984" MajorTopicYN="N">Epitopes, T-Lymphocyte</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020539" MajorTopicYN="N">Sequence Analysis, Protein</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013602" MajorTopicYN="N">T-Lymphocytes, Cytotoxic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22963340</ArticleId><ArticleId IdType="pii">1471-2172-13-50</ArticleId><ArticleId IdType="doi">10.1186/1471-2172-13-50</ArticleId><ArticleId IdType="pmc">PMC3575293</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Bioinformation. 2009 Jun 28;3(9):403-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19759816</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomed Microdevices. 2010 Apr;12(2):207-22</Citation><ArticleIdList><ArticleId IdType="pubmed">20033850</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Pathol. 2004 Mar;57(3):260-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14990596</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1996 Jun 1;156(11):4266-73</Citation><ArticleIdList><ArticleId IdType="pubmed">8666797</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2004 Dec 10;325(2):445-52</Citation><ArticleIdList><ArticleId IdType="pubmed">15530413</ArticleId></ArticleIdList></Reference><Reference><Citation>Int Immunol. 2009 Jan;21(1):63-71</Citation><ArticleIdList><ArticleId IdType="pubmed">19050106</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2004 Dec;34(4):436-43</Citation><ArticleIdList><ArticleId IdType="pubmed">15542369</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2004 Apr 1;428(6982):561-4</Citation><ArticleIdList><ArticleId IdType="pubmed">15024391</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2005 Apr 25;335(1):34-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15823604</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1999 Mar 26;274(13):8586-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10085093</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2010;11:568</Citation><ArticleIdList><ArticleId IdType="pubmed">21092157</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2004 Jul 1;104(1):200-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15016646</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1998 Nov 1;161(9):4719-27</Citation><ArticleIdList><ArticleId IdType="pubmed">9794402</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2003 Dec 1;171(11):5683-7</Citation><ArticleIdList><ArticleId IdType="pubmed">14634075</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2006;7:131</Citation><ArticleIdList><ArticleId IdType="pubmed">16533401</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1990 Sep 18;29(37):8509-17</Citation><ArticleIdList><ArticleId IdType="pubmed">2271534</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2009;10:296</Citation><ArticleIdList><ArticleId IdType="pubmed">19765293</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1994 Jan 1;152(1):163-75</Citation><ArticleIdList><ArticleId IdType="pubmed">8254189</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2003 Sep 22;19(14):1765-72</Citation><ArticleIdList><ArticleId IdType="pubmed">14512347</ArticleId></ArticleIdList></Reference><Reference><Citation>Org Lett. 2011 Feb 18;13(4):680-3</Citation><ArticleIdList><ArticleId IdType="pubmed">21235262</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunogenetics. 2005 Jun;57(5):304-14</Citation><ArticleIdList><ArticleId IdType="pubmed">15868141</ArticleId></ArticleIdList></Reference><Reference><Citation>Tissue Antigens. 2003 Nov;62(5):378-84</Citation><ArticleIdList><ArticleId IdType="pubmed">14617044</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2007 Aug 17;282(33):23799-810</Citation><ArticleIdList><ArticleId IdType="pubmed">17540778</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Comput Biol. 2006 Jun 9;2(6):e65</Citation><ArticleIdList><ArticleId IdType="pubmed">16789818</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 1995 May;63(5):1955-9</Citation><ArticleIdList><ArticleId IdType="pubmed">7537251</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2004 Aug 4;20 Suppl 1:i297-302</Citation><ArticleIdList><ArticleId IdType="pubmed">15262812</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 Apr 14;281(15):10618-25</Citation><ArticleIdList><ArticleId IdType="pubmed">16473882</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Chem. 2000 Jun 1;43(11):2135-48</Citation><ArticleIdList><ArticleId IdType="pubmed">10841792</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Immunol. 2008;9:8</Citation><ArticleIdList><ArticleId IdType="pubmed">18366636</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2007 Sep 28;25(39-40):6981-91</Citation><ArticleIdList><ArticleId IdType="pubmed">17709158</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W180-3</Citation><ArticleIdList><ArticleId IdType="pubmed">15980450</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 1995 Apr;13(6):581-91</Citation><ArticleIdList><ArticleId IdType="pubmed">7483779</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2005;6:132</Citation><ArticleIdList><ArticleId IdType="pubmed">15927070</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001308 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 001308 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:22963340
|texte= Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics prediction.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:22963340" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |