Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Identifieur interne : 000654 ( PubMed/Corpus ); précédent : 000653; suivant : 000655Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Auteurs : Regina Konrad ; Ute Eberle ; Alexandra Dangel ; Bianca Treis ; Anja Berger ; Katja Bengs ; Volker Fingerle ; Bernhard Liebl ; Nikolaus Ackermann ; Andreas SingSource :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
English descriptors
- KwdEn :
- Betacoronavirus (genetics), Betacoronavirus (isolation & purification), Clinical Laboratory Techniques, Coronavirus Infections (diagnosis), Coronavirus Infections (genetics), Germany, Humans, Oligonucleotide Array Sequence Analysis, Pneumonia, Viral (diagnosis), Pneumonia, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity, Time Factors, Viral Envelope Proteins (analysis), Viral Envelope Proteins (genetics), Workflow.
- MESH :
- chemical , analysis : Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- geographic : Germany.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus, Coronavirus Infections, Pneumonia, Viral.
- isolation & purification : Betacoronavirus.
- methods : Real-Time Polymerase Chain Reaction.
- Clinical Laboratory Techniques, Humans, Oligonucleotide Array Sequence Analysis, Sensitivity and Specificity, Time Factors, Workflow.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
Links to Exploration step
pubmed:32156330Le document en format XML
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<term>Oligonucleotide Array Sequence Analysis</term>
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<front><div type="abstract" xml:lang="en">The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</div>
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<Abstract><AbstractText>The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</AbstractText>
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