Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.
Identifieur interne : 002D66 ( PubMed/Checkpoint ); précédent : 002D65; suivant : 002D67Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.
Auteurs : Jyh-Lyh Juang [Taïwan] ; Tsan-Chi Chen ; Shih Sheng Jiang ; Chao A. Hsiung ; Wei-Chen Chen ; Guang-Wu Chen ; Shiang-Ming Lin ; Jih-Hui Lin ; Shu-Chun Chiu ; Yiu-Kay LaiSource :
- Laboratory investigation; a journal of technical methods and pathology [ 0023-6837 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN complémentaire (), ARN viral (analyse), Amorces ADN (), Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Séquençage par oligonucléotides en batterie (), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ARN viral.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- ARN complémentaire, Amorces ADN, Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- DNA Primers (chemistry), Humans, Oligonucleotide Array Sequence Analysis (methods), RNA, Complementary (chemistry), RNA, Viral (analysis), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : DNA Primers, RNA, Complementary.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Oligonucleotide Array Sequence Analysis.
- virology : Severe Acute Respiratory Syndrome.
- Humans, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.
DOI: 10.1038/labinvest.3700136
PubMed: 15195120
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:15195120Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.</title><author><name sortKey="Juang, Jyh Lyh" sort="Juang, Jyh Lyh" uniqKey="Juang J" first="Jyh-Lyh" last="Juang">Jyh-Lyh Juang</name><affiliation wicri:level="1"><nlm:affiliation>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan. juang@nhri.org.tw</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Tsan Chi" sort="Chen, Tsan Chi" uniqKey="Chen T" first="Tsan-Chi" last="Chen">Tsan-Chi Chen</name></author><author><name sortKey="Jiang, Shih Sheng" sort="Jiang, Shih Sheng" uniqKey="Jiang S" first="Shih Sheng" last="Jiang">Shih Sheng Jiang</name></author><author><name sortKey="Hsiung, Chao A" sort="Hsiung, Chao A" uniqKey="Hsiung C" first="Chao A" last="Hsiung">Chao A. Hsiung</name></author><author><name sortKey="Chen, Wei Chen" sort="Chen, Wei Chen" uniqKey="Chen W" first="Wei-Chen" last="Chen">Wei-Chen Chen</name></author><author><name sortKey="Chen, Guang Wu" sort="Chen, Guang Wu" uniqKey="Chen G" first="Guang-Wu" last="Chen">Guang-Wu Chen</name></author><author><name sortKey="Lin, Shiang Ming" sort="Lin, Shiang Ming" uniqKey="Lin S" first="Shiang-Ming" last="Lin">Shiang-Ming Lin</name></author><author><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name></author><author><name sortKey="Chiu, Shu Chun" sort="Chiu, Shu Chun" uniqKey="Chiu S" first="Shu-Chun" last="Chiu">Shu-Chun Chiu</name></author><author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15195120</idno><idno type="pmid">15195120</idno><idno type="doi">10.1038/labinvest.3700136</idno><idno type="wicri:Area/PubMed/Corpus">002D35</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D35</idno><idno type="wicri:Area/PubMed/Curation">002D35</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D35</idno><idno type="wicri:Area/PubMed/Checkpoint">002D66</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.</title><author><name sortKey="Juang, Jyh Lyh" sort="Juang, Jyh Lyh" uniqKey="Juang J" first="Jyh-Lyh" last="Juang">Jyh-Lyh Juang</name><affiliation wicri:level="1"><nlm:affiliation>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan. juang@nhri.org.tw</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Tsan Chi" sort="Chen, Tsan Chi" uniqKey="Chen T" first="Tsan-Chi" last="Chen">Tsan-Chi Chen</name></author><author><name sortKey="Jiang, Shih Sheng" sort="Jiang, Shih Sheng" uniqKey="Jiang S" first="Shih Sheng" last="Jiang">Shih Sheng Jiang</name></author><author><name sortKey="Hsiung, Chao A" sort="Hsiung, Chao A" uniqKey="Hsiung C" first="Chao A" last="Hsiung">Chao A. Hsiung</name></author><author><name sortKey="Chen, Wei Chen" sort="Chen, Wei Chen" uniqKey="Chen W" first="Wei-Chen" last="Chen">Wei-Chen Chen</name></author><author><name sortKey="Chen, Guang Wu" sort="Chen, Guang Wu" uniqKey="Chen G" first="Guang-Wu" last="Chen">Guang-Wu Chen</name></author><author><name sortKey="Lin, Shiang Ming" sort="Lin, Shiang Ming" uniqKey="Lin S" first="Shiang-Ming" last="Lin">Shiang-Ming Lin</name></author><author><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name></author><author><name sortKey="Chiu, Shu Chun" sort="Chiu, Shu Chun" uniqKey="Chiu S" first="Shu-Chun" last="Chiu">Shu-Chun Chiu</name></author><author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name></author></analytic><series><title level="j">Laboratory investigation; a journal of technical methods and pathology</title><idno type="ISSN">0023-6837</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Primers (chemistry)</term><term>Humans</term><term>Oligonucleotide Array Sequence Analysis (methods)</term><term>RNA, Complementary (chemistry)</term><term>RNA, Viral (analysis)</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN complémentaire ()</term><term>ARN viral (analyse)</term><term>Amorces ADN ()</term><term>Humains</term><term>RT-PCR</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquençage par oligonucléotides en batterie ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term><term>RNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Oligonucleotide Array Sequence Analysis</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN complémentaire</term><term>Amorces ADN</term><term>Humains</term><term>RT-PCR</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15195120</PMID><DateCompleted><Year>2004</Year><Month>09</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0023-6837</ISSN><JournalIssue CitedMedium="Print"><Volume>84</Volume><Issue>9</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Laboratory investigation; a journal of technical methods and pathology</Title><ISOAbbreviation>Lab. Invest.</ISOAbbreviation></Journal><ArticleTitle>Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.</ArticleTitle><Pagination><MedlinePgn>1085-91</MedlinePgn></Pagination><Abstract><AbstractText>An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Juang</LastName><ForeName>Jyh-Lyh</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei, Taiwan. juang@nhri.org.tw</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Tsan-Chi</ForeName><Initials>TC</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Shih Sheng</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Hsiung</LastName><ForeName>Chao A</ForeName><Initials>CA</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Wei-Chen</ForeName><Initials>WC</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Guang-Wu</ForeName><Initials>GW</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Shiang-Ming</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Jih-Hui</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Chiu</LastName><ForeName>Shu-Chun</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Yiu-Kay</ForeName><Initials>YK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Lab Invest</MedlineTA><NlmUniqueID>0376617</NlmUniqueID><ISSNLinking>0023-6837</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018075">RNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018075" MajorTopicYN="N">RNA, Complementary</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="Y">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>9</Month><Day>29</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>6</Month><Day>15</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15195120</ArticleId><ArticleId IdType="doi">10.1038/labinvest.3700136</ArticleId><ArticleId IdType="pii">3700136</ArticleId><ArticleId IdType="pmc">PMC7100734</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Lancet. 2003 May 24;361(9371):1767-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12781535</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Di Yi Jun Yi Da Xue Xue Bao. 2003 Jul;23(7):637-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12865207</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 1998 May;36(5):1388-91</Citation><ArticleIdList><ArticleId IdType="pubmed">9574711</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 1998 Nov;36(11):3149-54</Citation><ArticleIdList><ArticleId IdType="pubmed">9774555</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Microbiol Rev. 2000 Oct;13(4):559-70</Citation><ArticleIdList><ArticleId IdType="pubmed">11023957</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 1998 Jun;36(6):1544-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9620374</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jun;24(6):484-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12848915</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2002 Jan 30;82(1-2):65-71</Citation><ArticleIdList><ArticleId IdType="pubmed">11885953</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2004 Feb;10(2):304-10</Citation><ArticleIdList><ArticleId IdType="pubmed">15030702</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Trends Mol Med. 2003 Aug;9(8):323-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12928031</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Infect Dis. 2004 Jan 15;38(2):293-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14699465</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 1998 Oct;36(10):2990-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9738055</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 2001 Feb;39(2):696-704</Citation><ArticleIdList><ArticleId IdType="pubmed">11158130</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chen, Guang Wu" sort="Chen, Guang Wu" uniqKey="Chen G" first="Guang-Wu" last="Chen">Guang-Wu Chen</name><name sortKey="Chen, Tsan Chi" sort="Chen, Tsan Chi" uniqKey="Chen T" first="Tsan-Chi" last="Chen">Tsan-Chi Chen</name><name sortKey="Chen, Wei Chen" sort="Chen, Wei Chen" uniqKey="Chen W" first="Wei-Chen" last="Chen">Wei-Chen Chen</name><name sortKey="Chiu, Shu Chun" sort="Chiu, Shu Chun" uniqKey="Chiu S" first="Shu-Chun" last="Chiu">Shu-Chun Chiu</name><name sortKey="Hsiung, Chao A" sort="Hsiung, Chao A" uniqKey="Hsiung C" first="Chao A" last="Hsiung">Chao A. Hsiung</name><name sortKey="Jiang, Shih Sheng" sort="Jiang, Shih Sheng" uniqKey="Jiang S" first="Shih Sheng" last="Jiang">Shih Sheng Jiang</name><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><name sortKey="Lin, Shiang Ming" sort="Lin, Shiang Ming" uniqKey="Lin S" first="Shiang-Ming" last="Lin">Shiang-Ming Lin</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Juang, Jyh Lyh" sort="Juang, Jyh Lyh" uniqKey="Juang J" first="Jyh-Lyh" last="Juang">Jyh-Lyh Juang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D66 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002D66 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:15195120
|texte= Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:15195120" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |