Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.
Identifieur interne : 002D66 ( PubMed/Checkpoint ); précédent : 002D65; suivant : 002D67Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.
Auteurs : Jyh-Lyh Juang [Taïwan] ; Tsan-Chi Chen ; Shih Sheng Jiang ; Chao A. Hsiung ; Wei-Chen Chen ; Guang-Wu Chen ; Shiang-Ming Lin ; Jih-Hui Lin ; Shu-Chun Chiu ; Yiu-Kay LaiSource :
- Laboratory investigation; a journal of technical methods and pathology [ 0023-6837 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN complémentaire (), ARN viral (analyse), Amorces ADN (), Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Séquençage par oligonucléotides en batterie (), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ARN viral.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- ARN complémentaire, Amorces ADN, Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- DNA Primers (chemistry), Humans, Oligonucleotide Array Sequence Analysis (methods), RNA, Complementary (chemistry), RNA, Viral (analysis), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : DNA Primers, RNA, Complementary.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Oligonucleotide Array Sequence Analysis.
- virology : Severe Acute Respiratory Syndrome.
- Humans, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.
DOI: 10.1038/labinvest.3700136
PubMed: 15195120
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:15195120Le document en format XML
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<front><div type="abstract" xml:lang="en">An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.</div>
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