Identification of two antigenic epitopes on SARS-CoV spike protein.
Identifieur interne : 002C57 ( PubMed/Checkpoint ); précédent : 002C56; suivant : 002C58Identification of two antigenic epitopes on SARS-CoV spike protein.
Auteurs : Ronghong Hua [République populaire de Chine] ; Yanjun Zhou ; Yunfeng Wang ; Yuzhuo Hua ; Guangzhi TongSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (immunologie), Biologie informatique, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Peptides (génétique), Peptides (immunologie), Poulets, Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Séquence d'acides aminés, Virus du SRAS (immunologie), Épitopes (génétique).
- MESH :
- génétique : Glycoprotéines membranaires, Peptides, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Épitopes.
- immunologie : Anticorps monoclonaux, Glycoprotéines membranaires, Peptides, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Virus du SRAS.
- Animaux, Biologie informatique, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Poulets, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Chickens, Computational Biology, Epitopes (genetics), Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Molecular Sequence Data, Peptides (genetics), Peptides (immunology), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Epitopes, Membrane Glycoproteins, Peptides, Recombinant Fusion Proteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Peptides, Recombinant Fusion Proteins, Viral Envelope Proteins.
- immunology : SARS Virus.
- Amino Acid Sequence, Animals, Chickens, Computational Biology, Humans, Molecular Sequence Data, Spike Glycoprotein, Coronavirus.
Abstract
The spike (S) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. It plays an important role in interaction with receptor and inducing neutralizing antibodies. In the study, six tentative antigenic epitopes (S1 S2 S3 S4 S5 S6) of the spike protein of SARS-CoV were predicted by bio-informatics analysis, and a multi-epitope chimeric gene of S1-S2-S3-S4-S5-S6 was synthesized and fused to downstream GST gene in pGEX-6p-1. The Western blotting demonstrated that SARS patient convalescent serum could recognize the recombinant fusion protein. A number of monoclonal antibodies were developed against the fusion protein. In further, the six predicted epitope genes were individually fused to GST of pGEX-6p-1 and expressed in Escherichia coli BL21, respectively. Among six fusion peptides, S5 reacted with monoclonal antibody D3C5 and S2 reacted with monoclonal antibody D3D1 against spike protein of SARS-CoV. The epitopes recognized by monoclonal antibodies D3C5 and D3D1 are linear, and correspond to 447-458 and 789-799 amino acids of spike protein of SARS-CoV, respectively. Identification of antigenic epitope of spike protein of SARS-CoV could provide the basis for the development of immunity-based prophylactic, therapeutic, and diagnostic techniques for the control of severe acute respiratory syndrome.
DOI: 10.1016/j.bbrc.2004.05.066
PubMed: 15184071
Affiliations:
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Y" first="Yunfeng" last="Wang">Yunfeng Wang</name></author><author><name sortKey="Hua, Yuzhuo" sort="Hua, Yuzhuo" uniqKey="Hua Y" first="Yuzhuo" last="Hua">Yuzhuo Hua</name></author><author><name sortKey="Tong, Guangzhi" sort="Tong, Guangzhi" uniqKey="Tong G" first="Guangzhi" last="Tong">Guangzhi Tong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15184071</idno><idno type="pmid">15184071</idno><idno type="doi">10.1016/j.bbrc.2004.05.066</idno><idno type="wicri:Area/PubMed/Corpus">002D50</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D50</idno><idno type="wicri:Area/PubMed/Curation">002D50</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002D50</idno><idno type="wicri:Area/PubMed/Checkpoint">002C57</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C57</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of two antigenic epitopes on SARS-CoV spike protein.</title><author><name sortKey="Hua, Ronghong" sort="Hua, Ronghong" uniqKey="Hua R" first="Ronghong" last="Hua">Ronghong Hua</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Yanjun" sort="Zhou, Yanjun" uniqKey="Zhou Y" first="Yanjun" last="Zhou">Yanjun Zhou</name></author><author><name sortKey="Wang, Yunfeng" sort="Wang, Yunfeng" uniqKey="Wang Y" first="Yunfeng" last="Wang">Yunfeng Wang</name></author><author><name sortKey="Hua, Yuzhuo" sort="Hua, Yuzhuo" uniqKey="Hua Y" first="Yuzhuo" last="Hua">Yuzhuo Hua</name></author><author><name sortKey="Tong, Guangzhi" sort="Tong, Guangzhi" uniqKey="Tong G" first="Guangzhi" last="Tong">Guangzhi Tong</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Chickens</term><term>Computational Biology</term><term>Epitopes (genetics)</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Peptides (genetics)</term><term>Peptides (immunology)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Biologie informatique</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Peptides (génétique)</term><term>Peptides (immunologie)</term><term>Poulets</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (immunologie)</term><term>Épitopes (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes</term><term>Membrane Glycoproteins</term><term>Peptides</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Peptides</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chickens</term><term>Computational Biology</term><term>Humans</term><term>Molecular Sequence Data</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biologie informatique</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Poulets</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The spike (S) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. It plays an important role in interaction with receptor and inducing neutralizing antibodies. In the study, six tentative antigenic epitopes (S1 S2 S3 S4 S5 S6) of the spike protein of SARS-CoV were predicted by bio-informatics analysis, and a multi-epitope chimeric gene of S1-S2-S3-S4-S5-S6 was synthesized and fused to downstream GST gene in pGEX-6p-1. The Western blotting demonstrated that SARS patient convalescent serum could recognize the recombinant fusion protein. A number of monoclonal antibodies were developed against the fusion protein. In further, the six predicted epitope genes were individually fused to GST of pGEX-6p-1 and expressed in Escherichia coli BL21, respectively. Among six fusion peptides, S5 reacted with monoclonal antibody D3C5 and S2 reacted with monoclonal antibody D3D1 against spike protein of SARS-CoV. The epitopes recognized by monoclonal antibodies D3C5 and D3D1 are linear, and correspond to 447-458 and 789-799 amino acids of spike protein of SARS-CoV, respectively. Identification of antigenic epitope of spike protein of SARS-CoV could provide the basis for the development of immunity-based prophylactic, therapeutic, and diagnostic techniques for the control of severe acute respiratory syndrome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15184071</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>319</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Jul</Month><Day>02</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation></Journal><ArticleTitle>Identification of two antigenic epitopes on SARS-CoV spike protein.</ArticleTitle><Pagination><MedlinePgn>929-35</MedlinePgn></Pagination><Abstract><AbstractText>The spike (S) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. It plays an important role in interaction with receptor and inducing neutralizing antibodies. In the study, six tentative antigenic epitopes (S1 S2 S3 S4 S5 S6) of the spike protein of SARS-CoV were predicted by bio-informatics analysis, and a multi-epitope chimeric gene of S1-S2-S3-S4-S5-S6 was synthesized and fused to downstream GST gene in pGEX-6p-1. The Western blotting demonstrated that SARS patient convalescent serum could recognize the recombinant fusion protein. A number of monoclonal antibodies were developed against the fusion protein. In further, the six predicted epitope genes were individually fused to GST of pGEX-6p-1 and expressed in Escherichia coli BL21, respectively. Among six fusion peptides, S5 reacted with monoclonal antibody D3C5 and S2 reacted with monoclonal antibody D3D1 against spike protein of SARS-CoV. The epitopes recognized by monoclonal antibodies D3C5 and D3D1 are linear, and correspond to 447-458 and 789-799 amino acids of spike protein of SARS-CoV, respectively. Identification of antigenic epitope of spike protein of SARS-CoV could provide the basis for the development of immunity-based prophylactic, therapeutic, and diagnostic techniques for the control of severe acute respiratory syndrome.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Ronghong</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yanjun</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yunfeng</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Yuzhuo</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Guangzhi</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane 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MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="Y">Epitopes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence 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Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2001 Jan 20;279(2):371-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11162792</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Dec 26;312(4):1159-64</Citation><ArticleIdList><ArticleId IdType="pubmed">14651994</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1985 Sep;55(3):836-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2991600</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Chin Sci Bull. 2003;48(10):941-948</Citation><ArticleIdList><ArticleId IdType="pubmed">32214698</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Comput Appl Biosci. 1988 Mar;4(1):181-6</Citation><ArticleIdList><ArticleId IdType="pubmed">2454713</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 Nov 28;302(5650):1504-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14645828</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation><ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1982 May 5;157(1):105-32</Citation><ArticleIdList><ArticleId IdType="pubmed">7108955</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Hua, Yuzhuo" sort="Hua, Yuzhuo" uniqKey="Hua Y" first="Yuzhuo" last="Hua">Yuzhuo Hua</name><name sortKey="Tong, Guangzhi" sort="Tong, Guangzhi" uniqKey="Tong G" first="Guangzhi" last="Tong">Guangzhi Tong</name><name sortKey="Wang, Yunfeng" sort="Wang, Yunfeng" uniqKey="Wang Y" first="Yunfeng" last="Wang">Yunfeng Wang</name><name sortKey="Zhou, Yanjun" sort="Zhou, Yanjun" uniqKey="Zhou Y" first="Yanjun" last="Zhou">Yanjun Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Hua, Ronghong" sort="Hua, Ronghong" uniqKey="Hua R" first="Ronghong" last="Hua">Ronghong Hua</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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