Immunofluorescence assay for serologic diagnosis of SARS.
Identifieur interne : 002C55 ( PubMed/Checkpoint ); précédent : 002C54; suivant : 002C56Immunofluorescence assay for serologic diagnosis of SARS.
Auteurs : Paul K S. Chan [République populaire de Chine] ; King-Cheung Ng ; Rickjason C W. Chan ; Rebecca K Y. Lam ; Viola C Y. Chow ; Mamie Hui ; Alan Wu ; Nelson Lee ; Florence H Y. Yap ; Frankie W T. Cheng ; Joseph J Y. Sung ; John S. TamSource :
- Emerging infectious diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Anticorps antiviraux (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Hong Kong, Humains, Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (immunologie), Maladies transmissibles émergentes (physiopathologie), Mâle, RT-PCR, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (physiopathologie), Technique d'immunofluorescence indirecte ().
- MESH :
- diagnostic : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- immunologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- isolement et purification : Anticorps antiviraux.
- physiopathologie : Maladies transmissibles émergentes, Syndrome respiratoire aigu sévère.
- Adolescent, Adulte, Adulte d'âge moyen, Enfant, Enfant d'âge préscolaire, Femelle, Hong Kong, Humains, Mâle, RT-PCR, Technique d'immunofluorescence indirecte.
- Wicri :
- geographic : Hong Kong.
English descriptors
- KwdEn :
- Adolescent, Adult, Antibodies, Viral (isolation & purification), Child, Child, Preschool, Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (immunology), Communicable Diseases, Emerging (physiopathology), Female, Fluorescent Antibody Technique, Indirect (methods), Hong Kong, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (physiopathology).
- MESH :
- chemical , isolation & purification : Antibodies, Viral.
- geographic : Hong Kong.
- diagnosis : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- immunology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- methods : Fluorescent Antibody Technique, Indirect.
- physiopathology : Communicable Diseases, Emerging, Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
DOI: 10.3201/eid1003.030493
PubMed: 15109430
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:15109430Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunofluorescence assay for serologic diagnosis of SARS.</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name></author><author><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. Chan</name></author><author><name sortKey="Lam, Rebecca K Y" sort="Lam, Rebecca K Y" uniqKey="Lam R" first="Rebecca K Y" last="Lam">Rebecca K Y. Lam</name></author><author><name sortKey="Chow, Viola C Y" sort="Chow, Viola C Y" uniqKey="Chow V" first="Viola C Y" last="Chow">Viola C Y. Chow</name></author><author><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name></author><author><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name></author><author><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name></author><author><name sortKey="Yap, Florence H Y" sort="Yap, Florence H Y" uniqKey="Yap F" first="Florence H Y" last="Yap">Florence H Y. Yap</name></author><author><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15109430</idno><idno type="pmid">15109430</idno><idno type="doi">10.3201/eid1003.030493</idno><idno type="wicri:Area/PubMed/Corpus">002E33</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E33</idno><idno type="wicri:Area/PubMed/Curation">002E33</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E33</idno><idno type="wicri:Area/PubMed/Checkpoint">002C55</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002C55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunofluorescence assay for serologic diagnosis of SARS.</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name></author><author><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. Chan</name></author><author><name sortKey="Lam, Rebecca K Y" sort="Lam, Rebecca K Y" uniqKey="Lam R" first="Rebecca K Y" last="Lam">Rebecca K Y. Lam</name></author><author><name sortKey="Chow, Viola C Y" sort="Chow, Viola C Y" uniqKey="Chow V" first="Viola C Y" last="Chow">Viola C Y. Chow</name></author><author><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name></author><author><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name></author><author><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name></author><author><name sortKey="Yap, Florence H Y" sort="Yap, Florence H Y" uniqKey="Yap F" first="Florence H Y" last="Yap">Florence H Y. Yap</name></author><author><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="ISSN">1080-6040</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Antibodies, Viral (isolation & purification)</term><term>Child</term><term>Child, Preschool</term><term>Communicable Diseases, Emerging (diagnosis)</term><term>Communicable Diseases, Emerging (immunology)</term><term>Communicable Diseases, Emerging (physiopathology)</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect (methods)</term><term>Hong Kong</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (physiopathology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux (isolement et purification)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Hong Kong</term><term>Humains</term><term>Maladies transmissibles émergentes (diagnostic)</term><term>Maladies transmissibles émergentes (immunologie)</term><term>Maladies transmissibles émergentes (physiopathologie)</term><term>Mâle</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (physiopathologie)</term><term>Technique d'immunofluorescence indirecte ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Hong Kong</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluorescent Antibody Technique, Indirect</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Hong Kong</term><term>Humains</term><term>Mâle</term><term>RT-PCR</term><term>Technique d'immunofluorescence indirecte</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Hong Kong</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15109430</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1080-6040</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Emerging infectious diseases</Title><ISOAbbreviation>Emerging Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Immunofluorescence assay for serologic diagnosis of SARS.</ArticleTitle><Pagination><MedlinePgn>530-2</MedlinePgn></Pagination><Abstract><AbstractText>We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Faculty of Medicine, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, People's Republic of China. paulkschan@cuhk.edu.hk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>King-Cheung</ForeName><Initials>KC</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Rickjason C W</ForeName><Initials>RC</Initials></Author><Author ValidYN="Y"><LastName>Lam</LastName><ForeName>Rebecca K Y</ForeName><Initials>RK</Initials></Author><Author ValidYN="Y"><LastName>Chow</LastName><ForeName>Viola C Y</ForeName><Initials>VC</Initials></Author><Author ValidYN="Y"><LastName>Hui</LastName><ForeName>Mamie</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Alan</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Nelson</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Yap</LastName><ForeName>Florence H Y</ForeName><Initials>FH</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Frankie W T</ForeName><Initials>FW</Initials></Author><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>Joseph J Y</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Tam</LastName><ForeName>John S</ForeName><Initials>JS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Infect Dis</MedlineTA><NlmUniqueID>9508155</NlmUniqueID><ISSNLinking>1080-6040</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021821" MajorTopicYN="N">Communicable Diseases, Emerging</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006723" MajorTopicYN="N" Type="Geographic">Hong Kong</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>4</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>27</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>4</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15109430</ArticleId><ArticleId IdType="pmc">PMC3322771</ArticleId><ArticleId IdType="doi">10.3201/eid1003.030493</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1986-94</Citation><ArticleIdList><ArticleId IdType="pubmed">12682352</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2004 Feb;10(2):167-70</Citation><ArticleIdList><ArticleId IdType="pubmed">15040341</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Chan, Rickjason C W" sort="Chan, Rickjason C W" uniqKey="Chan R" first="Rickjason C W" last="Chan">Rickjason C W. Chan</name><name sortKey="Cheng, Frankie W T" sort="Cheng, Frankie W T" uniqKey="Cheng F" first="Frankie W T" last="Cheng">Frankie W T. Cheng</name><name sortKey="Chow, Viola C Y" sort="Chow, Viola C Y" uniqKey="Chow V" first="Viola C Y" last="Chow">Viola C Y. Chow</name><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name><name sortKey="Lam, Rebecca K Y" sort="Lam, Rebecca K Y" uniqKey="Lam R" first="Rebecca K Y" last="Lam">Rebecca K Y. Lam</name><name sortKey="Lee, Nelson" sort="Lee, Nelson" uniqKey="Lee N" first="Nelson" last="Lee">Nelson Lee</name><name sortKey="Ng, King Cheung" sort="Ng, King Cheung" uniqKey="Ng K" first="King-Cheung" last="Ng">King-Cheung Ng</name><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J Y" last="Sung">Joseph J Y. Sung</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name><name sortKey="Wu, Alan" sort="Wu, Alan" uniqKey="Wu A" first="Alan" last="Wu">Alan Wu</name><name sortKey="Yap, Florence H Y" sort="Yap, Florence H Y" uniqKey="Yap F" first="Florence H Y" last="Yap">Florence H Y. Yap</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C55 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002C55 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:15109430
|texte= Immunofluorescence assay for serologic diagnosis of SARS.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:15109430" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |