Molecular diagnosis of severe acute respiratory syndrome: the state of the art.
Identifieur interne : 002623 ( PubMed/Checkpoint ); précédent : 002622; suivant : 002624Molecular diagnosis of severe acute respiratory syndrome: the state of the art.
Auteurs : James B. Mahony [Canada] ; Susan RichardsonSource :
- The Journal of molecular diagnostics : JMD [ 1525-1578 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), ARN viral (isolement et purification), Animaux, Humains, Prélèvement biologique, RT-PCR, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Trousses de réactifs pour diagnostic, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ARN viral.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Virus du SRAS.
- isolement et purification : ARN viral, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Animaux, Humains, Prélèvement biologique, RT-PCR, Trousses de réactifs pour diagnostic.
English descriptors
- KwdEn :
- Animals, Humans, RNA, Viral (analysis), RNA, Viral (genetics), RNA, Viral (isolation & purification), Reagent Kits, Diagnostic, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Specimen Handling.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Humans, Reagent Kits, Diagnostic, Reverse Transcriptase Polymerase Chain Reaction, Specimen Handling.
Abstract
Severe acute respiratory syndrome (SARS) first appeared in Guangdong Province, China, in November 2002. Although virus isolation and serology were useful early in the SARS outbreak for diagnosing new cases, these tests are not generally useful because virus culture requires a BSL-3 laboratory and seroconversion is often delayed until 2 to 3 weeks after infection. The first qualitative reverse transcriptase-polymerase chain reaction tests for SARS-coronavirus (CoV) were sensitive and capable of detecting 1 to 10 genome equivalents. These assays were quickly supplemented with quantitative real-time assays that helped elucidate the natural history of SARS, particularly the initial presence of low viral loads in the upper respiratory tract and high viral loads in the lower respiratory tract. The unique natural history of SARS-CoV infection dictates the testing of both respiratory and nonrespiratory specimens, the testing of multiple specimens from the same patient, and sending out positives to be confirmed by a reference laboratory. Commercially available reverse transcriptase-polymerase chain reaction tests for SARS have recently appeared; however, meaningful evaluations of these assays have not yet been performed and their true performance has not been determined. These and other issues related to diagnosis of SARS-CoV infection are discussed in this review.
DOI: 10.1016/S1525-1578(10)60587-9
PubMed: 16258152
Affiliations:
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Mahony</name><affiliation wicri:level="1"><nlm:affiliation>Regional Virology Laboratory, St. Joseph's Hospital, 50 Charlton Ave. East, Hamilton, ON L8N 4A6, Canada. mahonyj@mcmaster.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Regional Virology Laboratory, St. Joseph's Hospital, 50 Charlton Ave. East, Hamilton, ON L8N 4A6</wicri:regionArea><wicri:noRegion>ON L8N 4A6</wicri:noRegion></affiliation></author><author><name sortKey="Richardson, Susan" sort="Richardson, Susan" uniqKey="Richardson S" first="Susan" last="Richardson">Susan Richardson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16258152</idno><idno type="pmid">16258152</idno><idno type="doi">10.1016/S1525-1578(10)60587-9</idno><idno type="wicri:Area/PubMed/Corpus">002471</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002471</idno><idno type="wicri:Area/PubMed/Curation">002471</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002471</idno><idno type="wicri:Area/PubMed/Checkpoint">002623</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002623</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular diagnosis of severe acute respiratory syndrome: the state of the art.</title><author><name sortKey="Mahony, James B" sort="Mahony, James B" uniqKey="Mahony J" first="James B" last="Mahony">James B. Mahony</name><affiliation wicri:level="1"><nlm:affiliation>Regional Virology Laboratory, St. Joseph's Hospital, 50 Charlton Ave. East, Hamilton, ON L8N 4A6, Canada. mahonyj@mcmaster.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Regional Virology Laboratory, St. Joseph's Hospital, 50 Charlton Ave. East, Hamilton, ON L8N 4A6</wicri:regionArea><wicri:noRegion>ON L8N 4A6</wicri:noRegion></affiliation></author><author><name sortKey="Richardson, Susan" sort="Richardson, Susan" uniqKey="Richardson S" first="Susan" last="Richardson">Susan Richardson</name></author></analytic><series><title level="j">The Journal of molecular diagnostics : JMD</title><idno type="ISSN">1525-1578</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Humans</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (isolation & purification)</term><term>Reagent Kits, Diagnostic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Specimen Handling</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>ARN viral (génétique)</term><term>ARN viral (isolement et purification)</term><term>Animaux</term><term>Humains</term><term>Prélèvement biologique</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Trousses de réactifs pour diagnostic</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Reagent Kits, Diagnostic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Specimen Handling</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Prélèvement biologique</term><term>RT-PCR</term><term>Trousses de réactifs pour diagnostic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) first appeared in Guangdong Province, China, in November 2002. Although virus isolation and serology were useful early in the SARS outbreak for diagnosing new cases, these tests are not generally useful because virus culture requires a BSL-3 laboratory and seroconversion is often delayed until 2 to 3 weeks after infection. The first qualitative reverse transcriptase-polymerase chain reaction tests for SARS-coronavirus (CoV) were sensitive and capable of detecting 1 to 10 genome equivalents. These assays were quickly supplemented with quantitative real-time assays that helped elucidate the natural history of SARS, particularly the initial presence of low viral loads in the upper respiratory tract and high viral loads in the lower respiratory tract. The unique natural history of SARS-CoV infection dictates the testing of both respiratory and nonrespiratory specimens, the testing of multiple specimens from the same patient, and sending out positives to be confirmed by a reference laboratory. Commercially available reverse transcriptase-polymerase chain reaction tests for SARS have recently appeared; however, meaningful evaluations of these assays have not yet been performed and their true performance has not been determined. These and other issues related to diagnosis of SARS-CoV infection are discussed in this review.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16258152</PMID><DateCompleted><Year>2006</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1525-1578</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>5</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The Journal of molecular diagnostics : JMD</Title><ISOAbbreviation>J Mol Diagn</ISOAbbreviation></Journal><ArticleTitle>Molecular diagnosis of severe acute respiratory syndrome: the state of the art.</ArticleTitle><Pagination><MedlinePgn>551-9</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) first appeared in Guangdong Province, China, in November 2002. Although virus isolation and serology were useful early in the SARS outbreak for diagnosing new cases, these tests are not generally useful because virus culture requires a BSL-3 laboratory and seroconversion is often delayed until 2 to 3 weeks after infection. The first qualitative reverse transcriptase-polymerase chain reaction tests for SARS-coronavirus (CoV) were sensitive and capable of detecting 1 to 10 genome equivalents. These assays were quickly supplemented with quantitative real-time assays that helped elucidate the natural history of SARS, particularly the initial presence of low viral loads in the upper respiratory tract and high viral loads in the lower respiratory tract. The unique natural history of SARS-CoV infection dictates the testing of both respiratory and nonrespiratory specimens, the testing of multiple specimens from the same patient, and sending out positives to be confirmed by a reference laboratory. Commercially available reverse transcriptase-polymerase chain reaction tests for SARS have recently appeared; however, meaningful evaluations of these assays have not yet been performed and their true performance has not been determined. 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Handling</DescriptorName></MeshHeading></MeshHeadingList><NumberOfReferences>34</NumberOfReferences></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>11</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>11</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16258152</ArticleId><ArticleId IdType="pii">S1525-1578(10)60587-9</ArticleId><ArticleId IdType="pmc">PMC1867551</ArticleId><ArticleId IdType="doi">10.1016/S1525-1578(10)60587-9</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Sex Transm Infect. 2002 Aug;78(4):232-4</Citation><ArticleIdList><ArticleId 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