[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].
Identifieur interne : 002360 ( PubMed/Checkpoint ); précédent : 002359; suivant : 002361[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].
Auteurs : Qing-Hua Hu [République populaire de Chine] ; Xiao-Li Liu ; Zhi-Xiong Zhuang ; Xiao-Lu Shi ; Yaqing He ; Jianjun Liu ; Bing Wang ; Tao Liu ; Yongyou Zhang ; Qingge Li ; Jin Zhao ; Jianfan He ; Hong Yang ; Shisong Fang ; Dan Zhang ; Jun'An ZhouSource :
- Wei sheng yan jiu = Journal of hygiene research [ 1000-8020 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Cadres ouverts de lecture, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Fèces, Pharynx, Sérum.
- Animaux, Chine, Humains, RT-PCR, Sensibilité et spécificité.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- MESH :
- geographic : China.
- genetics : Open Reading Frames, SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Feces, Pharynx, Serum.
- Animals, Humans, Sensitivity and Specificity.
Abstract
To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.
PubMed: 16229262
Affiliations:
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pubmed:16229262Le document en format XML
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Zhuang</name></author><author><name sortKey="Shi, Xiao Lu" sort="Shi, Xiao Lu" uniqKey="Shi X" first="Xiao-Lu" last="Shi">Xiao-Lu Shi</name></author><author><name sortKey="He, Yaqing" sort="He, Yaqing" uniqKey="He Y" first="Yaqing" last="He">Yaqing He</name></author><author><name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name></author><author><name sortKey="Wang, Bing" sort="Wang, Bing" uniqKey="Wang B" first="Bing" last="Wang">Bing Wang</name></author><author><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name></author><author><name sortKey="Zhang, Yongyou" sort="Zhang, Yongyou" uniqKey="Zhang Y" first="Yongyou" last="Zhang">Yongyou Zhang</name></author><author><name sortKey="Li, Qingge" sort="Li, Qingge" uniqKey="Li Q" first="Qingge" last="Li">Qingge Li</name></author><author><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name></author><author><name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name></author><author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name></author><author><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name></author><author><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name></author><author><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16229262</idno><idno type="pmid">16229262</idno><idno type="wicri:Area/PubMed/Corpus">002494</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002494</idno><idno 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type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liu, Xiao Li" sort="Liu, Xiao Li" uniqKey="Liu X" first="Xiao-Li" last="Liu">Xiao-Li Liu</name></author><author><name sortKey="Zhuang, Zhi Xiong" sort="Zhuang, Zhi Xiong" uniqKey="Zhuang Z" first="Zhi-Xiong" last="Zhuang">Zhi-Xiong Zhuang</name></author><author><name sortKey="Shi, Xiao Lu" sort="Shi, Xiao Lu" uniqKey="Shi X" first="Xiao-Lu" last="Shi">Xiao-Lu Shi</name></author><author><name sortKey="He, Yaqing" sort="He, Yaqing" uniqKey="He Y" first="Yaqing" last="He">Yaqing He</name></author><author><name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name></author><author><name sortKey="Wang, Bing" sort="Wang, Bing" uniqKey="Wang B" first="Bing" last="Wang">Bing Wang</name></author><author><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name></author><author><name sortKey="Zhang, Yongyou" sort="Zhang, Yongyou" uniqKey="Zhang Y" first="Yongyou" last="Zhang">Yongyou Zhang</name></author><author><name sortKey="Li, Qingge" sort="Li, Qingge" uniqKey="Li Q" first="Qingge" last="Li">Qingge Li</name></author><author><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name></author><author><name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name></author><author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name></author><author><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name></author><author><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name></author><author><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name></author></analytic><series><title level="j">Wei sheng yan jiu = Journal of hygiene research</title><idno type="ISSN">1000-8020</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>China</term><term>Feces (virology)</term><term>Humans</term><term>Open Reading Frames (genetics)</term><term>Pharynx (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Serum (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture (génétique)</term><term>Chine</term><term>Fèces (virologie)</term><term>Humains</term><term>Pharynx (virologie)</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Sérum (virologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term><term>Pharynx</term><term>Sérum</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term><term>Pharynx</term><term>Serum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chine</term><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16229262</PMID><DateCompleted><Year>2010</Year><Month>07</Month><Day>29</Day></DateCompleted><DateRevised><Year>2005</Year><Month>10</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-8020</ISSN><JournalIssue CitedMedium="Print"><Volume>34</Volume><Issue>4</Issue><PubDate><Year>2005</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Wei sheng yan jiu = Journal of hygiene research</Title><ISOAbbreviation>Wei Sheng Yan Jiu</ISOAbbreviation></Journal><ArticleTitle>[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].</ArticleTitle><Pagination><MedlinePgn>416-8</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">On the basis of the obtained core sequence of open reading frame 1b of the coronavirus polymerase gene sequences, which was published in GenBank, using modified molecular beacon probe, artifical virus techinique and two different fragments amplification with different fluoresce, one set of primers and probe were designed. Then fluorescent PCR assays for specific and sensitive detection of the SARS virus was established, while the ELISA & the traditional method were used as control. 368 clinical specimens such as the throat swab, serum, feces, and urine from different cases, 52 cell cultures and 50 animal specimens were detected by the molecular beacon-based PCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The sensitivity of real -time PCR was 10 - 100 copies/ml, there was no cross reaction to other respiratory viruses such as influenza virus etc. Of 368 specimens, 20 were positive by using molecular beacon-based fluorescence PCR. The positive rate of SARS case (10/47) were 21.27%, the positive rate of the throat swab of SARS cases (10/23) were 43.87% . Among 52 SARS cell cultures, 29 were positive. The positive rate of SARS cell cultures was 55.77% . Of 50 animal specimens, 23 were positive. The positive rate was 46%. Furthermore, SARS virus RNA was detected in feces and in serum during the acute phase.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The molecular beacon-based PCR is sensitive and specific, it could be applied to the early diagnosis and animal tracking. This molecular beacon-based PCR kit is useful for the different units.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Qing-Hua</ForeName><Initials>QH</Initials><AffiliationInfo><Affiliation>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xiao-Li</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>Zhuang</LastName><ForeName>Zhi-Xiong</ForeName><Initials>ZX</Initials></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Xiao-Lu</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Yaqing</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jianjun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Tao</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yongyou</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Qingge</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Jin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Jianfan</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Shisong</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Dan</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Jun'an</ForeName><Initials>J</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Yan Jiu</MedlineTA><NlmUniqueID>9426367</NlmUniqueID><ISSNLinking>1000-8020</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010614" MajorTopicYN="N">Pharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044967" MajorTopicYN="N">Serum</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16229262</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Shenzhen</li></settlement></list><tree><noCountry><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name><name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name><name sortKey="He, Yaqing" sort="He, Yaqing" uniqKey="He Y" first="Yaqing" last="He">Yaqing He</name><name sortKey="Li, Qingge" sort="Li, Qingge" uniqKey="Li Q" first="Qingge" last="Li">Qingge Li</name><name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name><name sortKey="Liu, Xiao Li" sort="Liu, Xiao Li" uniqKey="Liu X" first="Xiao-Li" last="Liu">Xiao-Li Liu</name><name sortKey="Shi, Xiao Lu" sort="Shi, Xiao Lu" uniqKey="Shi X" first="Xiao-Lu" last="Shi">Xiao-Lu Shi</name><name sortKey="Wang, Bing" sort="Wang, Bing" uniqKey="Wang B" first="Bing" last="Wang">Bing Wang</name><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name><name sortKey="Zhang, Yongyou" sort="Zhang, Yongyou" uniqKey="Zhang Y" first="Yongyou" last="Zhang">Yongyou Zhang</name><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name><name sortKey="Zhuang, Zhi Xiong" sort="Zhuang, Zhi Xiong" uniqKey="Zhuang Z" first="Zhi-Xiong" last="Zhuang">Zhi-Xiong Zhuang</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Hu, Qing Hua" sort="Hu, Qing Hua" uniqKey="Hu Q" first="Qing-Hua" last="Hu">Qing-Hua Hu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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