[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].
Identifieur interne : 002360 ( PubMed/Checkpoint ); précédent : 002359; suivant : 002361[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].
Auteurs : Qing-Hua Hu [République populaire de Chine] ; Xiao-Li Liu ; Zhi-Xiong Zhuang ; Xiao-Lu Shi ; Yaqing He ; Jianjun Liu ; Bing Wang ; Tao Liu ; Yongyou Zhang ; Qingge Li ; Jin Zhao ; Jianfan He ; Hong Yang ; Shisong Fang ; Dan Zhang ; Jun'An ZhouSource :
- Wei sheng yan jiu = Journal of hygiene research [ 1000-8020 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Cadres ouverts de lecture, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Fèces, Pharynx, Sérum.
- Animaux, Chine, Humains, RT-PCR, Sensibilité et spécificité.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- MESH :
- geographic : China.
- genetics : Open Reading Frames, SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Feces, Pharynx, Serum.
- Animals, Humans, Sensitivity and Specificity.
Abstract
To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.
PubMed: 16229262
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16229262Le document en format XML
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<author><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name>
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<author><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name>
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<term>China</term>
<term>Feces (virology)</term>
<term>Humans</term>
<term>Open Reading Frames (genetics)</term>
<term>Pharynx (virology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
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<term>Cadres ouverts de lecture (génétique)</term>
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<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Pharynx (virologie)</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Sérum (virologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term>
<term>SARS Virus</term>
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<term>Virus du SRAS</term>
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<term>Pharynx</term>
<term>Sérum</term>
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<front><div type="abstract" xml:lang="en">To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16229262</PMID>
<DateCompleted><Year>2010</Year>
<Month>07</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2005</Year>
<Month>10</Month>
<Day>18</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-8020</ISSN>
<JournalIssue CitedMedium="Print"><Volume>34</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Wei sheng yan jiu = Journal of hygiene research</Title>
<ISOAbbreviation>Wei Sheng Yan Jiu</ISOAbbreviation>
</Journal>
<ArticleTitle>[Modified molecular beacon-based dual real-time PCR for detection of SARS virus and its application].</ArticleTitle>
<Pagination><MedlinePgn>416-8</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop the modified molecular beacon-based dual fluorescent PCR assays for detection of SARS virus. The assay was applied to the early clinic diagnosis & animal tracking.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">On the basis of the obtained core sequence of open reading frame 1b of the coronavirus polymerase gene sequences, which was published in GenBank, using modified molecular beacon probe, artifical virus techinique and two different fragments amplification with different fluoresce, one set of primers and probe were designed. Then fluorescent PCR assays for specific and sensitive detection of the SARS virus was established, while the ELISA & the traditional method were used as control. 368 clinical specimens such as the throat swab, serum, feces, and urine from different cases, 52 cell cultures and 50 animal specimens were detected by the molecular beacon-based PCR.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The sensitivity of real -time PCR was 10 - 100 copies/ml, there was no cross reaction to other respiratory viruses such as influenza virus etc. Of 368 specimens, 20 were positive by using molecular beacon-based fluorescence PCR. The positive rate of SARS case (10/47) were 21.27%, the positive rate of the throat swab of SARS cases (10/23) were 43.87% . Among 52 SARS cell cultures, 29 were positive. The positive rate of SARS cell cultures was 55.77% . Of 50 animal specimens, 23 were positive. The positive rate was 46%. Furthermore, SARS virus RNA was detected in feces and in serum during the acute phase.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The molecular beacon-based PCR is sensitive and specific, it could be applied to the early diagnosis and animal tracking. This molecular beacon-based PCR kit is useful for the different units.</AbstractText>
</Abstract>
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<name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name>
<name sortKey="Liu, Xiao Li" sort="Liu, Xiao Li" uniqKey="Liu X" first="Xiao-Li" last="Liu">Xiao-Li Liu</name>
<name sortKey="Shi, Xiao Lu" sort="Shi, Xiao Lu" uniqKey="Shi X" first="Xiao-Lu" last="Shi">Xiao-Lu Shi</name>
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<name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name>
<name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name>
<name sortKey="Zhang, Yongyou" sort="Zhang, Yongyou" uniqKey="Zhang Y" first="Yongyou" last="Zhang">Yongyou Zhang</name>
<name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name>
<name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name>
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