[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].
Identifieur interne : 002353 ( PubMed/Checkpoint ); précédent : 002352; suivant : 002354[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].
Auteurs : Hai-Yan Wei [République populaire de Chine] ; Jian-Wei Wang ; Yang-Jing Ou ; Yan-Bin Wang ; Jian-Guo Qu ; Wei-Ming Zhao ; Tao HongSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules NIH 3T3, Cellules Vero, Escherichia coli (génétique), Escherichia coli (métabolisme), Fixation compétitive, Fragments peptidiques (), Fragments peptidiques (génétique), Fragments peptidiques (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Liaison aux protéines, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Récepteurs de surface cellulaire (métabolisme), Souris, Technique de Western, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Escherichia coli, Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- isolement et purification : Protéines recombinantes.
- métabolisme : Escherichia coli, Fragments peptidiques, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Récepteurs de surface cellulaire, Virus du SRAS.
- Animaux, Cellules NIH 3T3, Cellules Vero, Fixation compétitive, Fragments peptidiques, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Liaison aux protéines, Protéines de l'enveloppe virale, Souris, Technique de Western.
English descriptors
- KwdEn :
- Animals, Binding, Competitive, Blotting, Western, Chlorocebus aethiops, Escherichia coli (genetics), Escherichia coli (metabolism), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, NIH 3T3 Cells, Peptide Fragments (chemistry), Peptide Fragments (genetics), Peptide Fragments (metabolism), Protein Binding, Receptors, Cell Surface (metabolism), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- genetics : Escherichia coli, Membrane Glycoproteins, Peptide Fragments, SARS Virus, Viral Envelope Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- metabolism : Escherichia coli, Membrane Glycoproteins, Peptide Fragments, Receptors, Cell Surface, Recombinant Proteins, SARS Virus, Viral Envelope Proteins.
- Animals, Binding, Competitive, Blotting, Western, Chlorocebus aethiops, Mice, NIH 3T3 Cells, Protein Binding, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).
PubMed: 16415994
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16415994Le document en format XML
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<author><name sortKey="Ou, Yang Jing" sort="Ou, Yang Jing" uniqKey="Ou Y" first="Yang-Jing" last="Ou">Yang-Jing Ou</name>
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<author><name sortKey="Wang, Yan Bin" sort="Wang, Yan Bin" uniqKey="Wang Y" first="Yan-Bin" last="Wang">Yan-Bin Wang</name>
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<author><name sortKey="Qu, Jian Guo" sort="Qu, Jian Guo" uniqKey="Qu J" first="Jian-Guo" last="Qu">Jian-Guo Qu</name>
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<author><name sortKey="Zhao, Wei Ming" sort="Zhao, Wei Ming" uniqKey="Zhao W" first="Wei-Ming" last="Zhao">Wei-Ming Zhao</name>
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<term>Blotting, Western</term>
<term>Chlorocebus aethiops</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Mice</term>
<term>NIH 3T3 Cells</term>
<term>Peptide Fragments (chemistry)</term>
<term>Peptide Fragments (genetics)</term>
<term>Peptide Fragments (metabolism)</term>
<term>Protein Binding</term>
<term>Receptors, Cell Surface (metabolism)</term>
<term>Recombinant Proteins (isolation & purification)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules NIH 3T3</term>
<term>Cellules Vero</term>
<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Fixation compétitive</term>
<term>Fragments peptidiques ()</term>
<term>Fragments peptidiques (génétique)</term>
<term>Fragments peptidiques (métabolisme)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Liaison aux protéines</term>
<term>Protéines de l'enveloppe virale ()</term>
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<term>Protéines recombinantes (métabolisme)</term>
<term>Récepteurs de surface cellulaire (métabolisme)</term>
<term>Souris</term>
<term>Technique de Western</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term>
<term>Fragments peptidiques</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
<term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>Receptors, Cell Surface</term>
<term>Recombinant Proteins</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term>
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<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Récepteurs de surface cellulaire</term>
<term>Virus du SRAS</term>
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<term>Binding, Competitive</term>
<term>Blotting, Western</term>
<term>Chlorocebus aethiops</term>
<term>Mice</term>
<term>NIH 3T3 Cells</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules NIH 3T3</term>
<term>Cellules Vero</term>
<term>Fixation compétitive</term>
<term>Fragments peptidiques</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Liaison aux protéines</term>
<term>Protéines de l'enveloppe virale</term>
<term>Souris</term>
<term>Technique de Western</term>
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<front><div type="abstract" xml:lang="en">To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).</div>
</front>
</TEI>
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<DateCompleted><Year>2008</Year>
<Month>12</Month>
<Day>22</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN>
<JournalIssue CitedMedium="Print"><Volume>19</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title>
<ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].</ArticleTitle>
<Pagination><MedlinePgn>353-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Two different fragments S260-600 and S397-796 of the SARS-CoV S protein were expressed in Escherichia coli (E.coli) using a pET expression vector, respectively. The two recombinant proteins were separately verified by Western blot, purified by nickel-affinity chromatography, and incubated with Vero cells, a susceptible cell line of SARS-CoV infection, for cell binding assay. After the sequential probing with sera from convalescent SARS-patients and FITC-labeled anti-human IgG, the cells were analyzed by flow cytometry. The NIH 3T3 cell, a non-permissive cell line of SARS-CoV infection, was used as controls.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The recombinant proteins S260-600 and S397-796 were efficiently expressed in an insoluble form in E.coli. The appropriate expression of the proteins was confirmed by Western blotting using both SARS patients' sera and anti-6 x histidine antibody. The flow cytometry results showed that the both proteins were able to bind Vero cells, but the binding ability of S260-600 was somewhat stronger than that of S397-796. In contrast, the S260-600 protein did not bind NIH3T3 cells.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Both S260-600 and S397-796 exhibited different receptor binding activity. The S260-600 fragment probably contains the important receptor binding domain and could be a potential candidate for the development of SARS vaccine and anti-SARS therapeutics.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName>
<ForeName>Hai-yan</ForeName>
<Initials>HY</Initials>
<AffiliationInfo><Affiliation>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.</Affiliation>
</AffiliationInfo>
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<ForeName>Jian-wei</ForeName>
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<ForeName>Yang-jing</ForeName>
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<ForeName>Yan-bin</ForeName>
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<ForeName>Wei-ming</ForeName>
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<ISSNLinking>1003-9279</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
</Chemical>
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<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
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</Chemical>
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<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
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<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
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<MeshHeading><DescriptorName UI="D041681" MajorTopicYN="N">NIH 3T3 Cells</DescriptorName>
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<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
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<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<name sortKey="Ou, Yang Jing" sort="Ou, Yang Jing" uniqKey="Ou Y" first="Yang-Jing" last="Ou">Yang-Jing Ou</name>
<name sortKey="Qu, Jian Guo" sort="Qu, Jian Guo" uniqKey="Qu J" first="Jian-Guo" last="Qu">Jian-Guo Qu</name>
<name sortKey="Wang, Jian Wei" sort="Wang, Jian Wei" uniqKey="Wang J" first="Jian-Wei" last="Wang">Jian-Wei Wang</name>
<name sortKey="Wang, Yan Bin" sort="Wang, Yan Bin" uniqKey="Wang Y" first="Yan-Bin" last="Wang">Yan-Bin Wang</name>
<name sortKey="Zhao, Wei Ming" sort="Zhao, Wei Ming" uniqKey="Zhao W" first="Wei-Ming" last="Zhao">Wei-Ming Zhao</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Wei, Hai Yan" sort="Wei, Hai Yan" uniqKey="Wei H" first="Hai-Yan" last="Wei">Hai-Yan Wei</name>
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