[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].
Identifieur interne : 002378 ( PubMed/Corpus ); précédent : 002377; suivant : 002379[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].
Auteurs : Hai-Yan Wei ; Jian-Wei Wang ; Yang-Jing Ou ; Yan-Bin Wang ; Jian-Guo Qu ; Wei-Ming Zhao ; Tao HongSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Binding, Competitive, Blotting, Western, Chlorocebus aethiops, Escherichia coli (genetics), Escherichia coli (metabolism), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, NIH 3T3 Cells, Peptide Fragments (chemistry), Peptide Fragments (genetics), Peptide Fragments (metabolism), Protein Binding, Receptors, Cell Surface (metabolism), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins.
- genetics : Escherichia coli, Membrane Glycoproteins, Peptide Fragments, SARS Virus, Viral Envelope Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- metabolism : Escherichia coli, Membrane Glycoproteins, Peptide Fragments, Receptors, Cell Surface, Recombinant Proteins, SARS Virus, Viral Envelope Proteins.
- Animals, Binding, Competitive, Blotting, Western, Chlorocebus aethiops, Mice, NIH 3T3 Cells, Protein Binding, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).
PubMed: 16415994
Links to Exploration step
pubmed:16415994Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].</title>
<author><name sortKey="Wei, Hai Yan" sort="Wei, Hai Yan" uniqKey="Wei H" first="Hai-Yan" last="Wei">Hai-Yan Wei</name>
<affiliation><nlm:affiliation>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Wang, Jian Wei" sort="Wang, Jian Wei" uniqKey="Wang J" first="Jian-Wei" last="Wang">Jian-Wei Wang</name>
</author>
<author><name sortKey="Ou, Yang Jing" sort="Ou, Yang Jing" uniqKey="Ou Y" first="Yang-Jing" last="Ou">Yang-Jing Ou</name>
</author>
<author><name sortKey="Wang, Yan Bin" sort="Wang, Yan Bin" uniqKey="Wang Y" first="Yan-Bin" last="Wang">Yan-Bin Wang</name>
</author>
<author><name sortKey="Qu, Jian Guo" sort="Qu, Jian Guo" uniqKey="Qu J" first="Jian-Guo" last="Qu">Jian-Guo Qu</name>
</author>
<author><name sortKey="Zhao, Wei Ming" sort="Zhao, Wei Ming" uniqKey="Zhao W" first="Wei-Ming" last="Zhao">Wei-Ming Zhao</name>
</author>
<author><name sortKey="Hong, Tao" sort="Hong, Tao" uniqKey="Hong T" first="Tao" last="Hong">Tao Hong</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].</title>
<author><name sortKey="Wei, Hai Yan" sort="Wei, Hai Yan" uniqKey="Wei H" first="Hai-Yan" last="Wei">Hai-Yan Wei</name>
<affiliation><nlm:affiliation>Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.</nlm:affiliation>
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<author><name sortKey="Wang, Jian Wei" sort="Wang, Jian Wei" uniqKey="Wang J" first="Jian-Wei" last="Wang">Jian-Wei Wang</name>
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<author><name sortKey="Ou, Yang Jing" sort="Ou, Yang Jing" uniqKey="Ou Y" first="Yang-Jing" last="Ou">Yang-Jing Ou</name>
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<author><name sortKey="Wang, Yan Bin" sort="Wang, Yan Bin" uniqKey="Wang Y" first="Yan-Bin" last="Wang">Yan-Bin Wang</name>
</author>
<author><name sortKey="Qu, Jian Guo" sort="Qu, Jian Guo" uniqKey="Qu J" first="Jian-Guo" last="Qu">Jian-Guo Qu</name>
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<author><name sortKey="Zhao, Wei Ming" sort="Zhao, Wei Ming" uniqKey="Zhao W" first="Wei-Ming" last="Zhao">Wei-Ming Zhao</name>
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<author><name sortKey="Hong, Tao" sort="Hong, Tao" uniqKey="Hong T" first="Tao" last="Hong">Tao Hong</name>
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<series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title>
<idno type="ISSN">1003-9279</idno>
<imprint><date when="2005" type="published">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Binding, Competitive</term>
<term>Blotting, Western</term>
<term>Chlorocebus aethiops</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Mice</term>
<term>NIH 3T3 Cells</term>
<term>Peptide Fragments (chemistry)</term>
<term>Peptide Fragments (genetics)</term>
<term>Peptide Fragments (metabolism)</term>
<term>Protein Binding</term>
<term>Receptors, Cell Surface (metabolism)</term>
<term>Recombinant Proteins (isolation & purification)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
<term>Membrane Glycoproteins</term>
<term>Peptide Fragments</term>
<term>Receptors, Cell Surface</term>
<term>Recombinant Proteins</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Binding, Competitive</term>
<term>Blotting, Western</term>
<term>Chlorocebus aethiops</term>
<term>Mice</term>
<term>NIH 3T3 Cells</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
</keywords>
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<front><div type="abstract" xml:lang="en">To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16415994</PMID>
<DateCompleted><Year>2008</Year>
<Month>12</Month>
<Day>22</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN>
<JournalIssue CitedMedium="Print"><Volume>19</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title>
<ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Receptor-binding ability of fragments 260-600 and 397-796 of SARS-associated coronavirus spike protein].</ArticleTitle>
<Pagination><MedlinePgn>353-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">To investigate the interaction between the host cell and the truncated S fragments to identify the receptor-binding domain of the spike (S) protein of SARS-associated coronavirus (SARS-CoV).</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Two different fragments S260-600 and S397-796 of the SARS-CoV S protein were expressed in Escherichia coli (E.coli) using a pET expression vector, respectively. The two recombinant proteins were separately verified by Western blot, purified by nickel-affinity chromatography, and incubated with Vero cells, a susceptible cell line of SARS-CoV infection, for cell binding assay. After the sequential probing with sera from convalescent SARS-patients and FITC-labeled anti-human IgG, the cells were analyzed by flow cytometry. The NIH 3T3 cell, a non-permissive cell line of SARS-CoV infection, was used as controls.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The recombinant proteins S260-600 and S397-796 were efficiently expressed in an insoluble form in E.coli. The appropriate expression of the proteins was confirmed by Western blotting using both SARS patients' sera and anti-6 x histidine antibody. The flow cytometry results showed that the both proteins were able to bind Vero cells, but the binding ability of S260-600 was somewhat stronger than that of S397-796. In contrast, the S260-600 protein did not bind NIH3T3 cells.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Both S260-600 and S397-796 exhibited different receptor binding activity. The S260-600 fragment probably contains the important receptor binding domain and could be a potential candidate for the development of SARS vaccine and anti-SARS therapeutics.</AbstractText>
</Abstract>
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<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Jian-wei</ForeName>
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<Author ValidYN="Y"><LastName>Ou</LastName>
<ForeName>Yang-jing</ForeName>
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<Author ValidYN="Y"><LastName>Wang</LastName>
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<Author ValidYN="Y"><LastName>Qu</LastName>
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<Author ValidYN="Y"><LastName>Zhao</LastName>
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<Author ValidYN="Y"><LastName>Hong</LastName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA>
<NlmUniqueID>9602873</NlmUniqueID>
<ISSNLinking>1003-9279</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance>
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<MeshHeading><DescriptorName UI="D001667" MajorTopicYN="N">Binding, Competitive</DescriptorName>
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<MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
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<MeshHeading><DescriptorName UI="D011956" MajorTopicYN="N">Receptors, Cell Surface</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
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</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
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