SrasV1, PubMed, Checkpoint, bibRecord, 002275

Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.

Identifieur interne : 002275 ( PubMed/Checkpoint ); précédent : 002274; suivant : 002276

Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.

Auteurs : Y. Liao [Singapour] ; Q. Yuan ; J. Torres ; J P Tam ; D X Liu

Source :

Virology [ 0042-6822 ] ; 2006.

RBID : pubmed:16507314

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Amino Acid Substitution, Animals, Cell Line, Cell Membrane (metabolism), Cell Membrane Permeability, Cricetinae, DNA Mutational Analysis, Endoplasmic Reticulum (metabolism), Golgi Apparatus (metabolism), HeLa Cells, Humans, Immunohistochemistry, Membrane Microdomains (metabolism), Microscopy, Fluorescence, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Missense, Palmitoyl Coenzyme A (metabolism), Protein Processing, Post-Translational, Protein Structure, Tertiary, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Envelope Proteins (physiology).
MESH :
- chemical , chemistry : Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- chemical , metabolism : Palmitoyl Coenzyme A, Viral Envelope Proteins.
- metabolism : Cell Membrane, Endoplasmic Reticulum, Golgi Apparatus, Membrane Microdomains.
- chemical , physiology : Viral Envelope Proteins.
- Amino Acid Sequence, Amino Acid Substitution, Animals, Cell Line, Cell Membrane Permeability, Cricetinae, DNA Mutational Analysis, HeLa Cells, Humans, Immunohistochemistry, Microscopy, Fluorescence, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Missense, Protein Processing, Post-Translational, Protein Structure, Tertiary.

Abstract

A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.

DOI: 10.1016/j.virol.2006.01.028
PubMed: 16507314

Affiliations:

Singapour

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002326
to stream PubMed, to step Curation: 002326

Links to Exploration step

pubmed:16507314

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.</title>
<author><name sortKey="Liao, Y" sort="Liao, Y" uniqKey="Liao Y" first="Y" last="Liao">Y. Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuan, Q" sort="Yuan, Q" uniqKey="Yuan Q" first="Q" last="Yuan">Q. Yuan</name>
</author>
<author><name sortKey="Torres, J" sort="Torres, J" uniqKey="Torres J" first="J" last="Torres">J. Torres</name>
</author>
<author><name sortKey="Tam, J P" sort="Tam, J P" uniqKey="Tam J" first="J P" last="Tam">J P Tam</name>
</author>
<author><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D X" last="Liu">D X Liu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16507314</idno>
<idno type="pmid">16507314</idno>
<idno type="doi">10.1016/j.virol.2006.01.028</idno>
<idno type="wicri:Area/PubMed/Corpus">002326</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002326</idno>
<idno type="wicri:Area/PubMed/Curation">002326</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002326</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002275</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002275</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.</title>
<author><name sortKey="Liao, Y" sort="Liao, Y" uniqKey="Liao Y" first="Y" last="Liao">Y. Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551</wicri:regionArea>
<wicri:noRegion>Singapore 637551</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuan, Q" sort="Yuan, Q" uniqKey="Yuan Q" first="Q" last="Yuan">Q. Yuan</name>
</author>
<author><name sortKey="Torres, J" sort="Torres, J" uniqKey="Torres J" first="J" last="Torres">J. Torres</name>
</author>
<author><name sortKey="Tam, J P" sort="Tam, J P" uniqKey="Tam J" first="J P" last="Tam">J P Tam</name>
</author>
<author><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D X" last="Liu">D X Liu</name>
</author>
</analytic>
<series><title level="j">Virology</title>
<idno type="ISSN">0042-6822</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Amino Acid Substitution</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Cell Membrane (metabolism)</term>
<term>Cell Membrane Permeability</term>
<term>Cricetinae</term>
<term>DNA Mutational Analysis</term>
<term>Endoplasmic Reticulum (metabolism)</term>
<term>Golgi Apparatus (metabolism)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Membrane Microdomains (metabolism)</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis, Site-Directed</term>
<term>Mutation, Missense</term>
<term>Palmitoyl Coenzyme A (metabolism)</term>
<term>Protein Processing, Post-Translational</term>
<term>Protein Structure, Tertiary</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Envelope Proteins (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de mutations d'ADN</term>
<term>Animaux</term>
<term>Appareil de Golgi (métabolisme)</term>
<term>Cellules HeLa</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Lignée cellulaire</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Microdomaines membranaires (métabolisme)</term>
<term>Microscopie de fluorescence</term>
<term>Mutagenèse dirigée</term>
<term>Mutation faux-sens</term>
<term>Palmitoyl coenzyme A (métabolisme)</term>
<term>Perméabilité de la membrane cellulaire</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Réticulum endoplasmique (métabolisme)</term>
<term>Structure tertiaire des protéines</term>
<term>Substitution d'acide aminé</term>
<term>Séquence d'acides aminés</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Palmitoyl Coenzyme A</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Endoplasmic Reticulum</term>
<term>Golgi Apparatus</term>
<term>Membrane Microdomains</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Appareil de Golgi</term>
<term>Membrane cellulaire</term>
<term>Microdomaines membranaires</term>
<term>Palmitoyl coenzyme A</term>
<term>Protéines de l'enveloppe virale</term>
<term>Réticulum endoplasmique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Amino Acid Substitution</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Cell Membrane Permeability</term>
<term>Cricetinae</term>
<term>DNA Mutational Analysis</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Microscopy, Fluorescence</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis, Site-Directed</term>
<term>Mutation, Missense</term>
<term>Protein Processing, Post-Translational</term>
<term>Protein Structure, Tertiary</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de mutations d'ADN</term>
<term>Animaux</term>
<term>Cellules HeLa</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Lignée cellulaire</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Microscopie de fluorescence</term>
<term>Mutagenèse dirigée</term>
<term>Mutation faux-sens</term>
<term>Perméabilité de la membrane cellulaire</term>
<term>Protéines de l'enveloppe virale</term>
<term>Structure tertiaire des protéines</term>
<term>Substitution d'acide aminé</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16507314</PMID>
<DateCompleted><Year>2006</Year>
<Month>07</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0042-6822</ISSN>
<JournalIssue CitedMedium="Print"><Volume>349</Volume>
<Issue>2</Issue>
<PubDate><Year>2006</Year>
<Month>Jun</Month>
<Day>05</Day>
</PubDate>
</JournalIssue>
<Title>Virology</Title>
<ISOAbbreviation>Virology</ISOAbbreviation>
</Journal>
<ArticleTitle>Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.</ArticleTitle>
<Pagination><MedlinePgn>264-75</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A diverse group of cytolytic animal viruses encodes small, hydrophobic proteins to modify host cell membrane permeability to ions and small molecules during their infection cycles. In this study, we show that expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells. Immunofluorescent staining and cell fractionation studies demonstrate that this protein is an integral membrane protein. It is mainly localized to the ER and the Golgi apparatus. The protein can be translocated to the cell surface and is partially associated with lipid rafts. Further biochemical characterization of the protein reveals that it is posttranslationally modified by palmitoylation on all three cysteine residues. Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the SARS-CoV E protein is associated with its transmembrane domain.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liao</LastName>
<ForeName>Y</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yuan</LastName>
<ForeName>Q</ForeName>
<Initials>Q</Initials>
</Author>
<Author ValidYN="Y"><LastName>Torres</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tam</LastName>
<ForeName>J P</ForeName>
<Initials>JP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>D X</ForeName>
<Initials>DX</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2006</Year>
<Month>02</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Virology</MedlineTA>
<NlmUniqueID>0110674</NlmUniqueID>
<ISSNLinking>0042-6822</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C501689">E protein, SARS coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>1763-10-6</RegistryNumber>
<NameOfSubstance UI="D010171">Palmitoyl Coenzyme A</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002463" MajorTopicYN="Y">Cell Membrane Permeability</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004721" MajorTopicYN="N">Endoplasmic Reticulum</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006056" MajorTopicYN="N">Golgi Apparatus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006367" MajorTopicYN="N">HeLa Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D021962" MajorTopicYN="N">Membrane Microdomains</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020125" MajorTopicYN="N">Mutation, Missense</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010171" MajorTopicYN="N">Palmitoyl Coenzyme A</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011499" MajorTopicYN="N">Protein Processing, Post-Translational</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year>
<Month>11</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2005</Year>
<Month>12</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2006</Year>
<Month>01</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>3</Month>
<Day>2</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>7</Month>
<Day>15</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>3</Month>
<Day>2</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16507314</ArticleId>
<ArticleId IdType="pii">S0042-6822(06)00056-0</ArticleId>
<ArticleId IdType="doi">10.1016/j.virol.2006.01.028</ArticleId>
<ArticleId IdType="pmc">PMC7111751</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2002 Dec;76(23):11845-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12414927</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Mar;79(5):3182-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15709039</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Jan;73(1):251-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9847328</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1997 Mar;71(3):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9032311</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Eur Biophys J. 2005 Feb;34(1):52-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15221235</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Trends Cell Biol. 2003 Jan;13(1):32-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12480338</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1994 Apr 22;269(16):12106-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8163515</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2004 May;78(10):5500-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15113929</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 1996 Nov 25;398(1):12-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8946945</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 1991 Jun;72 ( Pt 6):1461-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2045796</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1988 Dec 15;263(35):18635-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3143715</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 2005 Jul 4;579(17):3607-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15963987</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1998 Dec 5;252(1):218-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9875331</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2001 May 18;276(20):17515-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11278557</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 May;74(10):4634-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10775599</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochemistry. 1998 Sep 29;37(39):13710-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9753459</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Virus Res. 1995;45:61-112</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7793329</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biophys J. 2005 Feb;88(2):1283-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15713601</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1994 Aug 1;202(2):1018-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8030202</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 1993 Dec 27;336(2):243-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8262238</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Mol Biol. 2004 Aug 13;341(3):769-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15288785</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2003 Sep;84(Pt 9):2305-2315</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell. 1992 May 1;69(3):517-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1374685</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2003 Jul 20;312(1):25-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12890618</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1989 Jul 25;264(21):12590-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2545712</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2002 Oct 25;277(43):40434-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12183456</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Dec 3;325(1):374-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15522242</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2004 Dec 5;330(1):322-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15527857</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Feb;76(3):1273-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11773403</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1998 Oct;72(10):7885-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9733825</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13523-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11095714</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1991 Dec;185(2):911-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1962461</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 2003 Sep 18;552(1):28-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12972148</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1999 Feb 5;274(6):3910-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9920947</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1989 Jan;168(1):173-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2535902</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 2003 Sep 18;552(1):7-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12972144</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Dec;75(23):11544-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11689636</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 May;74(9):4319-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10756047</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 May 13;100(10):6104-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12719519</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2005 May;109(2):191-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15763150</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1998 May 10;244(2):343-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9601505</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1996 Sep 20;271(38):23134-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8798506</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2002 May 17;277(20):17789-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11893756</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Singapour</li>
</country>
</list>
<tree><noCountry><name sortKey="Liu, D X" sort="Liu, D X" uniqKey="Liu D" first="D X" last="Liu">D X Liu</name>
<name sortKey="Tam, J P" sort="Tam, J P" uniqKey="Tam J" first="J P" last="Tam">J P Tam</name>
<name sortKey="Torres, J" sort="Torres, J" uniqKey="Torres J" first="J" last="Torres">J. Torres</name>
<name sortKey="Yuan, Q" sort="Yuan, Q" uniqKey="Yuan Q" first="Q" last="Yuan">Q. Yuan</name>
</noCountry>
<country name="Singapour"><noRegion><name sortKey="Liao, Y" sort="Liao, Y" uniqKey="Liao Y" first="Y" last="Liao">Y. Liao</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002275 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002275 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:16507314
   |texte=   Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:16507314" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.

Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki