SrasV1, PubMed, Checkpoint, bibRecord, 002017

SARS-associated coronavirus replication in cell lines.

Identifieur interne : 002017 ( PubMed/Checkpoint ); précédent : 002016; suivant : 002018

SARS-associated coronavirus replication in cell lines.

Auteurs : Matthew Kaye [Australie]

Source :

Emerging infectious diseases [ 1080-6040 ] ; 2006.

RBID : pubmed:16494729

Descripteurs français

KwdFr :
- Animaux, Chiens, Effet cytopathogène viral, Haplorhini (virologie), Humains, Lapins, Lignée cellulaire, Réplication virale (physiologie), Sensibilité et spécificité, Souris, Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- génétique : Virus du SRAS.
- physiologie : Réplication virale, Virus du SRAS.
- virologie : Haplorhini.
- Animaux, Chiens, Effet cytopathogène viral, Humains, Lapins, Lignée cellulaire, Sensibilité et spécificité, Souris.

English descriptors

KwdEn :
- Animals, Cell Line, Cytopathogenic Effect, Viral, Dogs, Haplorhini (virology), Humans, Mice, Rabbits, SARS Virus (genetics), SARS Virus (physiology), Sensitivity and Specificity, Virus Replication (physiology).
MESH :
- genetics : SARS Virus.
- physiology : SARS Virus, Virus Replication.
- virology : Haplorhini.
- Animals, Cell Line, Cytopathogenic Effect, Viral, Dogs, Humans, Mice, Rabbits, Sensitivity and Specificity.

Abstract

Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.

DOI: 10.3201/eid1201.050496
PubMed: 16494729

Affiliations:

Australie

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pubmed:16494729

Le document en format XML

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<affiliation wicri:level="1"><nlm:affiliation>Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia. matthew.kaye@mh.org.au</nlm:affiliation>
<country xml:lang="fr">Australie</country>
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<term>Dogs</term>
<term>Haplorhini (virology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Rabbits</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Chiens</term>
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<term>Haplorhini (virologie)</term>
<term>Humains</term>
<term>Lapins</term>
<term>Lignée cellulaire</term>
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</keywords>
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<term>Virus du SRAS</term>
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<term>Cell Line</term>
<term>Cytopathogenic Effect, Viral</term>
<term>Dogs</term>
<term>Humans</term>
<term>Mice</term>
<term>Rabbits</term>
<term>Sensitivity and Specificity</term>
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<term>Chiens</term>
<term>Effet cytopathogène viral</term>
<term>Humains</term>
<term>Lapins</term>
<term>Lignée cellulaire</term>
<term>Sensibilité et spécificité</term>
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<front><div type="abstract" xml:lang="en">Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.</div>
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SARS-associated coronavirus replication in cell lines.

SARS-associated coronavirus replication in cell lines.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki