SrasV1, PubMed, Checkpoint, bibRecord, 001C39

Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).

Identifieur interne : 001C39 ( PubMed/Checkpoint ); précédent : 001C38; suivant : 001C40

Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).

Auteurs : Zhongping He [République populaire de Chine] ; Hui Zhuang ; Chunhui Zhao ; Qingming Dong ; Guoai Peng ; Dominic E. Dwyer

Source :

Virology journal [ 1743-422X ] ; 2007.

RBID : pubmed:17386116

Descripteurs français

KwdFr :
- ARN viral (analyse), ARN viral (génétique), Adulte, Adulte d'âge moyen, Fèces (virologie), Humains, Pharynx (virologie), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), RT-PCR, Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Sérum (virologie), Virus du SRAS (isolement et purification).
MESH :
- analyse : ARN viral.
- génétique : ARN viral.
- isolement et purification : Virus du SRAS.
- virologie : Fèces, Pharynx, Pneumopathie virale, Syndrome respiratoire aigu sévère, Sérum.
- épidémiologie : Pneumopathie virale, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Humains, RT-PCR.

English descriptors

KwdEn :
- Adult, Feces (virology), Humans, Middle Aged, Pharynx (virology), Pneumonia, Viral (epidemiology), Pneumonia, Viral (virology), RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (isolation & purification), Serum (virology), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology).
MESH :
- chemical , analysis : RNA, Viral.
- epidemiology : Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- chemical , genetics : RNA, Viral.
- isolation & purification : SARS Virus.
- virology : Feces, Pharynx, Pneumonia, Viral, Serum, Severe Acute Respiratory Syndrome.
- Adult, Humans, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.

DOI: 10.1186/1743-422X-4-32
PubMed: 17386116

Affiliations:

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pubmed:17386116

Le document en format XML

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<affiliation wicri:level="3"><nlm:affiliation>Beijing Ditan Hospital, Beijing, People's Republic of China. zhongpinghe@yahoo.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<placeName><settlement type="city">Pékin</settlement>
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<author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
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<author><name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
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<author><name sortKey="Dong, Qingming" sort="Dong, Qingming" uniqKey="Dong Q" first="Qingming" last="Dong">Qingming Dong</name>
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<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
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<author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
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<author><name sortKey="Dong, Qingming" sort="Dong, Qingming" uniqKey="Dong Q" first="Qingming" last="Dong">Qingming Dong</name>
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<author><name sortKey="Peng, Guoai" sort="Peng, Guoai" uniqKey="Peng G" first="Guoai" last="Peng">Guoai Peng</name>
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<term>Feces (virology)</term>
<term>Humans</term>
<term>Middle Aged</term>
<term>Pharynx (virology)</term>
<term>Pneumonia, Viral (epidemiology)</term>
<term>Pneumonia, Viral (virology)</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (isolation & purification)</term>
<term>Serum (virology)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>ARN viral (génétique)</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Fèces (virologie)</term>
<term>Humains</term>
<term>Pharynx (virologie)</term>
<term>Pneumopathie virale (virologie)</term>
<term>Pneumopathie virale (épidémiologie)</term>
<term>RT-PCR</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Syndrome respiratoire aigu sévère (épidémiologie)</term>
<term>Sérum (virologie)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<term>Severe Acute Respiratory Syndrome</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term>
<term>Pharynx</term>
<term>Pneumopathie virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Sérum</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
<term>Pharynx</term>
<term>Pneumonia, Viral</term>
<term>Serum</term>
<term>Severe Acute Respiratory Syndrome</term>
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<term>Adulte d'âge moyen</term>
<term>Humains</term>
<term>RT-PCR</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17386116</PMID>
<DateCompleted><Year>2007</Year>
<Month>06</Month>
<Day>18</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
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<JournalIssue CitedMedium="Internet"><Volume>4</Volume>
<PubDate><Year>2007</Year>
<Month>Mar</Month>
<Day>27</Day>
</PubDate>
</JournalIssue>
<Title>Virology journal</Title>
<ISOAbbreviation>Virol. J.</ISOAbbreviation>
</Journal>
<ArticleTitle>Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).</ArticleTitle>
<Pagination><MedlinePgn>32</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome (SARS) caused a large outbreak of pneumonia in Beijing, China, in 2003. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect and quantify SARS-CoV in 934 sera and self-collected throat washes and fecal samples from 271 patients with laboratory-confirmed SARS managed at a single institution.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">SARS-CoV detection rates in sera were highest in the first 9 days of illness, whereas detection was highest in throat washes 5-14 days after onset of symptoms. The highest SARS-CoV RT-PCR rates (70.4-86.3%) and viral loads (log10 4.5-6.1) were seen in fecal samples collected 2-4 weeks after the onset of clinical illness. Fecal samples were frequently SARS-CoV RT-PCR positive beyond 40 days, and occasional sera still had SARS-CoV detected after 3 weeks of illness.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">In the context of an extensive outbreak with major pressure on hospital resources, patient self-collected samples are an alternative to nasopharyngeal aspirates for laboratory confirmation of SARS-CoV infection.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Zhongping</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Beijing Ditan Hospital, Beijing, People's Republic of China. zhongpinghe@yahoo.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhuang</LastName>
<ForeName>Hui</ForeName>
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<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Chunhui</ForeName>
<Initials>C</Initials>
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<Author ValidYN="Y"><LastName>Dong</LastName>
<ForeName>Qingming</ForeName>
<Initials>Q</Initials>
</Author>
<Author ValidYN="Y"><LastName>Peng</LastName>
<ForeName>Guoai</ForeName>
<Initials>G</Initials>
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<Author ValidYN="Y"><LastName>Dwyer</LastName>
<ForeName>Dominic E</ForeName>
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<Language>eng</Language>
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<name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E" last="Dwyer">Dominic E. Dwyer</name>
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<name sortKey="Zhao, Chunhui" sort="Zhao, Chunhui" uniqKey="Zhao C" first="Chunhui" last="Zhao">Chunhui Zhao</name>
<name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name>
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Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).

Using patient-collected clinical samples and sera to detect and quantify the severe acute respiratory syndrome coronavirus (SARS-CoV).

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Descripteurs français

English descriptors

Abstract

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