Protein transfer enhances cellular immune responses to DNA vaccination against SARS-CoV.
Identifieur interne : 001811 ( PubMed/Checkpoint ); précédent : 001810; suivant : 001812Protein transfer enhances cellular immune responses to DNA vaccination against SARS-CoV.
Auteurs : Youmin Kang [République populaire de Chine] ; Aoshuang Chen ; Bin Wang ; Guoxing ZhengSource :
- Viral immunology [ 1557-8976 ] ; 2009.
Descripteurs français
- KwdFr :
- Acide palmitique (administration et posologie), Activation des lymphocytes, Adjuvants immunologiques (administration et posologie), Animaux, Anticorps antiviraux (biosynthèse), Cellules cultivées (immunologie), Cellules présentatrices d'antigène (immunologie), Cytotoxicité immunologique, Fragments Fc des immunoglobulines (immunologie), Humains, Hypersensibilité retardée (immunologie), Immunité cellulaire, Immunoconjugués (administration et posologie), Immunoconjugués (immunologie), Immunoglobuline G (immunologie), Ligand de 4-1BB (administration et posologie), Ligand de 4-1BB (immunologie), Lymphocytes T cytotoxiques (immunologie), Protéine A staphylococcique (administration et posologie), Protéine A staphylococcique (immunologie), Protéines de fusion recombinantes (immunologie), Protéines nucléocapside (immunologie), Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Vaccination, Vaccins à ADN (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Acide palmitique, Adjuvants immunologiques, Immunoconjugués, Ligand de 4-1BB, Protéine A staphylococcique.
- biosynthèse : Anticorps antiviraux.
- immunologie : Cellules cultivées, Cellules présentatrices d'antigène, Fragments Fc des immunoglobulines, Hypersensibilité retardée, Immunoconjugués, Immunoglobuline G, Ligand de 4-1BB, Lymphocytes T cytotoxiques, Protéine A staphylococcique, Protéines de fusion recombinantes, Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- Activation des lymphocytes, Animaux, Cytotoxicité immunologique, Humains, Immunité cellulaire, Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Vaccination.
English descriptors
- KwdEn :
- 4-1BB Ligand (administration & dosage), 4-1BB Ligand (immunology), Adjuvants, Immunologic (administration & dosage), Animals, Antibodies, Viral (biosynthesis), Antigen-Presenting Cells (immunology), Cells, Cultured (immunology), Cytotoxicity, Immunologic, Humans, Hypersensitivity, Delayed (immunology), Immunity, Cellular, Immunoconjugates (administration & dosage), Immunoconjugates (immunology), Immunoglobulin Fc Fragments (immunology), Immunoglobulin G (immunology), Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Nucleocapsid Proteins (immunology), Palmitic Acid (administration & dosage), Recombinant Fusion Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Staphylococcal Protein A (administration & dosage), Staphylococcal Protein A (immunology), T-Lymphocytes, Cytotoxic (immunology), Vaccination, Vaccines, DNA (immunology).
- MESH :
- chemical , administration & dosage : 4-1BB Ligand, Adjuvants, Immunologic, Immunoconjugates, Palmitic Acid, Staphylococcal Protein A.
- chemical , biosynthesis : Antibodies, Viral.
- chemical , immunology : 4-1BB Ligand, Immunoconjugates, Immunoglobulin Fc Fragments, Immunoglobulin G, Nucleocapsid Proteins, Recombinant Fusion Proteins, Staphylococcal Protein A, Vaccines, DNA.
- immunology : Antigen-Presenting Cells, Cells, Cultured, Hypersensitivity, Delayed, SARS Virus, T-Lymphocytes, Cytotoxic.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cytotoxicity, Immunologic, Humans, Immunity, Cellular, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Vaccination.
Abstract
The current DNA vaccine formulations are not optimal for stimulation of CD8(+) T cells, which are required for clearing virally-infected cells. Here we show that CD8(+) T cell-stimulating activity can be effectively augmented by combining DNA vaccination with protein transfer. C57BL/6 mice were injected intramuscularly with an anti-SARS-CoV DNA vaccine admixed with a lipid-derived conjugate of 4-1BBL, a potential CD8(+) T-cell co-stimulator. The inclusion of the lipidated co-stimulator greatly enhanced cellular immune responses, especially the CTL response, induced by the DNA vaccine. The adjuvant effect of 4-1BBL was lipidation-dependent, indicating that it functions as a cell membrane-anchored co-stimulator. Results of our study suggest, for the first time, that muscle cells may be modified in situ, at the DNA injection site, into APC-like cells to allow direct priming of CD8(+) T cells and thereby improve the efficacy of DNA vaccines.
DOI: 10.1089/vim.2009.0048
PubMed: 19951178
Affiliations:
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pubmed:19951178Le document en format XML
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<term>Antigen-Presenting Cells (immunology)</term>
<term>Cells, Cultured (immunology)</term>
<term>Cytotoxicity, Immunologic</term>
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<term>Immunity, Cellular</term>
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<term>Immunoconjugates (immunology)</term>
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<term>Mice, Inbred C57BL</term>
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<term>Cellules présentatrices d'antigène (immunologie)</term>
<term>Cytotoxicité immunologique</term>
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<term>Cytotoxicity, Immunologic</term>
<term>Humans</term>
<term>Immunity, Cellular</term>
<term>Lymphocyte Activation</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
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<term>Vaccination</term>
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<term>Cytotoxicité immunologique</term>
<term>Humains</term>
<term>Immunité cellulaire</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Souris de lignée C57BL</term>
<term>Syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">The current DNA vaccine formulations are not optimal for stimulation of CD8(+) T cells, which are required for clearing virally-infected cells. Here we show that CD8(+) T cell-stimulating activity can be effectively augmented by combining DNA vaccination with protein transfer. C57BL/6 mice were injected intramuscularly with an anti-SARS-CoV DNA vaccine admixed with a lipid-derived conjugate of 4-1BBL, a potential CD8(+) T-cell co-stimulator. The inclusion of the lipidated co-stimulator greatly enhanced cellular immune responses, especially the CTL response, induced by the DNA vaccine. The adjuvant effect of 4-1BBL was lipidation-dependent, indicating that it functions as a cell membrane-anchored co-stimulator. Results of our study suggest, for the first time, that muscle cells may be modified in situ, at the DNA injection site, into APC-like cells to allow direct priming of CD8(+) T cells and thereby improve the efficacy of DNA vaccines.</div>
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<Abstract><AbstractText>The current DNA vaccine formulations are not optimal for stimulation of CD8(+) T cells, which are required for clearing virally-infected cells. Here we show that CD8(+) T cell-stimulating activity can be effectively augmented by combining DNA vaccination with protein transfer. C57BL/6 mice were injected intramuscularly with an anti-SARS-CoV DNA vaccine admixed with a lipid-derived conjugate of 4-1BBL, a potential CD8(+) T-cell co-stimulator. The inclusion of the lipidated co-stimulator greatly enhanced cellular immune responses, especially the CTL response, induced by the DNA vaccine. The adjuvant effect of 4-1BBL was lipidation-dependent, indicating that it functions as a cell membrane-anchored co-stimulator. Results of our study suggest, for the first time, that muscle cells may be modified in situ, at the DNA injection site, into APC-like cells to allow direct priming of CD8(+) T cells and thereby improve the efficacy of DNA vaccines.</AbstractText>
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<tree><noCountry><name sortKey="Chen, Aoshuang" sort="Chen, Aoshuang" uniqKey="Chen A" first="Aoshuang" last="Chen">Aoshuang Chen</name>
<name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name>
<name sortKey="Zheng, Guoxing" sort="Zheng, Guoxing" uniqKey="Zheng G" first="Guoxing" last="Zheng">Guoxing Zheng</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Kang, Youmin" sort="Kang, Youmin" uniqKey="Kang Y" first="Youmin" last="Kang">Youmin Kang</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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