Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses.
Identifieur interne : 000D85 ( PubMed/Checkpoint ); précédent : 000D84; suivant : 000D86Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses.
Auteurs : Mozhdeh Sultani ; Talat Mokhtari Azad ; Mohammadreza Eshragian ; Azadeh Shadab ; Maryam Naseri ; Owrang Eilami ; Jila YavarianSource :
- Jundishapur journal of microbiology [ 2008-3645 ] ; 2015.
Abstract
It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.
DOI: 10.5812/jjm.19041v2
PubMed: 26468358
Affiliations:
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uniqKey="Eshragian M" first="Mohammadreza" last="Eshragian">Mohammadreza Eshragian</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Shadab, Azadeh" sort="Shadab, Azadeh" uniqKey="Shadab A" first="Azadeh" last="Shadab">Azadeh Shadab</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Naseri, Maryam" sort="Naseri, Maryam" uniqKey="Naseri M" first="Maryam" last="Naseri">Maryam Naseri</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Eilami, Owrang" sort="Eilami, Owrang" uniqKey="Eilami O" first="Owrang" last="Eilami">Owrang Eilami</name><affiliation><nlm:affiliation>Department of Infectious Disease, Yasuj University of Medical Sciences, Yasuj, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Yavarian, Jila" sort="Yavarian, Jila" uniqKey="Yavarian J" first="Jila" last="Yavarian">Jila Yavarian</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26468358</idno><idno type="pmid">26468358</idno><idno type="doi">10.5812/jjm.19041v2</idno><idno 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Talat" sort="Mokhtari Azad, Talat" uniqKey="Mokhtari Azad T" first="Talat" last="Mokhtari Azad">Talat Mokhtari Azad</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Eshragian, Mohammadreza" sort="Eshragian, Mohammadreza" uniqKey="Eshragian M" first="Mohammadreza" last="Eshragian">Mohammadreza Eshragian</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Shadab, Azadeh" sort="Shadab, Azadeh" uniqKey="Shadab A" first="Azadeh" last="Shadab">Azadeh Shadab</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Naseri, Maryam" sort="Naseri, Maryam" uniqKey="Naseri M" first="Maryam" last="Naseri">Maryam Naseri</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Eilami, Owrang" sort="Eilami, Owrang" uniqKey="Eilami O" first="Owrang" last="Eilami">Owrang Eilami</name><affiliation><nlm:affiliation>Department of Infectious Disease, Yasuj University of Medical Sciences, Yasuj, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Yavarian, Jila" sort="Yavarian, Jila" uniqKey="Yavarian J" first="Jila" last="Yavarian">Jila Yavarian</name><affiliation><nlm:affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author></analytic><series><title level="j">Jundishapur journal of microbiology</title><idno type="ISSN">2008-3645</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26468358</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2008-3645</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Jundishapur journal of microbiology</Title><ISOAbbreviation>Jundishapur J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses.</ArticleTitle><Pagination><MedlinePgn>e19041</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5812/jjm.19041v2</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR Green real-time polymerase chain reaction (PCR).</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR Green multiplex real-time PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Application of the multiplex SYBR Green real-time PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012).</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Multiplex SYBR Green real-time PCR is a rapid and relatively inexpensive method for detection of respiratory viruses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sultani</LastName><ForeName>Mozhdeh</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mokhtari Azad</LastName><ForeName>Talat</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eshragian</LastName><ForeName>Mohammadreza</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shadab</LastName><ForeName>Azadeh</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naseri</LastName><ForeName>Maryam</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eilami</LastName><ForeName>Owrang</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Infectious Disease, Yasuj University of Medical Sciences, Yasuj, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yavarian</LastName><ForeName>Jila</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Iran</Country><MedlineTA>Jundishapur J Microbiol</MedlineTA><NlmUniqueID>101515122</NlmUniqueID><ISSNLinking>2008-3645</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Real-Time PCR</Keyword><Keyword MajorTopicYN="N">Respiratory Syncytial Viruses</Keyword><Keyword MajorTopicYN="N">SYBR 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IdType="pubmed">20554810</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Eilami, Owrang" sort="Eilami, Owrang" uniqKey="Eilami O" first="Owrang" last="Eilami">Owrang Eilami</name><name sortKey="Eshragian, Mohammadreza" sort="Eshragian, Mohammadreza" uniqKey="Eshragian M" first="Mohammadreza" last="Eshragian">Mohammadreza Eshragian</name><name sortKey="Mokhtari Azad, Talat" sort="Mokhtari Azad, Talat" uniqKey="Mokhtari Azad T" first="Talat" last="Mokhtari Azad">Talat Mokhtari Azad</name><name sortKey="Naseri, Maryam" sort="Naseri, Maryam" uniqKey="Naseri M" first="Maryam" last="Naseri">Maryam Naseri</name><name sortKey="Shadab, Azadeh" sort="Shadab, Azadeh" uniqKey="Shadab A" first="Azadeh" last="Shadab">Azadeh Shadab</name><name sortKey="Sultani, Mozhdeh" sort="Sultani, Mozhdeh" uniqKey="Sultani M" first="Mozhdeh" last="Sultani">Mozhdeh Sultani</name><name sortKey="Yavarian, Jila" sort="Yavarian, Jila" uniqKey="Yavarian J" first="Jila" last="Yavarian">Jila Yavarian</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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