In vitro susceptibility of 10 clinical isolates of SARS coronavirus to selected antiviral compounds
Identifieur interne : 000890 ( Pmc/Curation ); précédent : 000889; suivant : 000891In vitro susceptibility of 10 clinical isolates of SARS coronavirus to selected antiviral compounds
Auteurs : F. Chen [Hong Kong] ; K. H Chan [Hong Kong] ; Y. Jiang [Hong Kong] ; R. Y. T Kao [Hong Kong] ; H. T Lu [Hong Kong] ; K. W Fan [Hong Kong] ; V. C. C Cheng [Hong Kong] ; W. H. W Tsui [Hong Kong] ; I. F. N Hung [Hong Kong] ; T. S. W Lee [Hong Kong] ; Y. Guan [Hong Kong] ; J. S. M Peiris [Hong Kong] ; K. Y Yuen [Hong Kong]Source :
- Journal of Clinical Virology [ 1386-6532 ] ; 2004.
Abstract
Effective antiviral agents are urgently needed to combat the possible return of severe acute respiratory syndrome (SARS). Commercial antiviral agents and pure chemical compounds extracted from traditional Chinese medicinal herbs were screened against 10 clinical isolates of SARS coronavirus by neutralisation tests with confirmation by plaque reduction assays. Interferon-beta-1a, leukocytic interferon-alpha, ribavirin, lopinavir, rimantadine, baicalin and glycyrrhizin showed antiviral activity. The two interferons were only active if the cell lines were pre-incubated with the drugs 16 h before viral inoculation. Results were confirmed by plaque reduction assays. Antiviral activity varied with the use of different cell lines. Checkerboard assays for synergy were performed showing combinations of interferon beta-1a or leukocytic interferon-alpha with ribavirin are synergistic. Since the clinical and toxicity profiles of these agents are well known, they should be considered either singly or in combination for prophylaxis or treatment of SARS in randomised placebo controlled trials in future epidemics.
Url:
DOI: 10.1016/j.jcv.2004.03.003
PubMed: 15288617
PubMed Central: 7128415
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Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road</wicri:regionArea></affiliation></author><author><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K. H" last="Chan">K. 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T Lu</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road</wicri:regionArea></affiliation></author><author><name sortKey="Fan, K W" sort="Fan, K W" uniqKey="Fan K" first="K. W" last="Fan">K. W Fan</name><affiliation wicri:level="1"><nlm:aff id="AFF1">Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road</wicri:regionArea></affiliation></author><author><name sortKey="Cheng, V C C" sort="Cheng, V C C" uniqKey="Cheng V" first="V. C. C" last="Cheng">V. C. C Cheng</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Tsui, W H W" sort="Tsui, W H W" uniqKey="Tsui W" first="W. H. W" last="Tsui">W. H. W Tsui</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Hung, I F N" sort="Hung, I F N" uniqKey="Hung I" first="I. F. N" last="Hung">I. F. N Hung</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Lee, T S W" sort="Lee, T S W" uniqKey="Lee T" first="T. S. W" last="Lee">T. S. W Lee</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Guan, Y" sort="Guan, Y" uniqKey="Guan Y" first="Y" last="Guan">Y. Guan</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M" last="Peiris">J. S. M Peiris</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K. Y" last="Yuen">K. Y Yuen</name><affiliation wicri:level="1"><nlm:aff id="AFF2">Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</nlm:aff><country xml:lang="fr">Hong Kong</country><wicri:regionArea>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Clinical Virology</title><idno type="ISSN">1386-6532</idno><idno type="eISSN">1873-5967</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Effective antiviral agents are urgently needed to combat the possible return of severe acute respiratory syndrome (SARS). Commercial antiviral agents and pure chemical compounds extracted from traditional Chinese medicinal herbs were screened against 10 clinical isolates of SARS coronavirus by neutralisation tests with confirmation by plaque reduction assays. Interferon-beta-1a, leukocytic interferon-alpha, ribavirin, lopinavir, rimantadine, baicalin and glycyrrhizin showed antiviral activity. The two interferons were only active if the cell lines were pre-incubated with the drugs 16 h before viral inoculation. Results were confirmed by plaque reduction assays. Antiviral activity varied with the use of different cell lines. Checkerboard assays for synergy were performed showing combinations of interferon beta-1a or leukocytic interferon-alpha with ribavirin are synergistic. Since the clinical and toxicity profiles of these agents are well known, they should be considered either singly or in combination for prophylaxis or treatment of SARS in randomised placebo controlled trials in future epidemics.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name></author><author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name></author><author><name sortKey="Bauer, G" uniqKey="Bauer G">G Bauer</name></author><author><name sortKey="Chandra, P" uniqKey="Chandra P">P Chandra</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name></author><author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name></author><author><name sortKey="Bauer, G" uniqKey="Bauer G">G Bauer</name></author><author><name sortKey="Chandra, P" uniqKey="Chandra P">P Chandra</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M Chu</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C Cheng</name></author><author><name sortKey="Hung, I F" uniqKey="Hung I">I.F Hung</name></author><author><name sortKey="Wong, M M" uniqKey="Wong M">M.M Wong</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Chan, K S" uniqKey="Chan K">K.S Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donnelly, C A" uniqKey="Donnelly C">C.A Donnelly</name></author><author><name sortKey="Ghani, A C" uniqKey="Ghani A">A.C Ghani</name></author><author><name sortKey="Leung, G M" uniqKey="Leung G">G.M Leung</name></author><author><name sortKey="Hedley, A J" uniqKey="Hedley A">A.J Hedley</name></author><author><name sortKey="Fraser, C" uniqKey="Fraser C">C Fraser</name></author><author><name sortKey="Riley, S" uniqKey="Riley S">S Riley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Douglas, R M" uniqKey="Douglas R">R.M Douglas</name></author><author><name sortKey="Moore, B W" uniqKey="Moore B">B.W Moore</name></author><author><name sortKey="Miles, H B" uniqKey="Miles H">H.B Miles</name></author><author><name sortKey="Davies, L M" uniqKey="Davies L">L.M Davies</name></author><author><name sortKey="Graham, N M" uniqKey="Graham N">N.M Graham</name></author><author><name sortKey="Ryan, P" uniqKey="Ryan P">P Ryan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eron, L J" uniqKey="Eron L">L.J Eron</name></author><author><name sortKey="Toy, C" uniqKey="Toy C">C Toy</name></author><author><name sortKey="Salsitz, B" uniqKey="Salsitz B">B Salsitz</name></author><author><name sortKey="Scheer, R R" uniqKey="Scheer R">R.R Scheer</name></author><author><name sortKey="Wood, D L" uniqKey="Wood D">D.L Wood</name></author><author><name sortKey="Nadler, P I" uniqKey="Nadler P">P.I Nadler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, S X" uniqKey="Fan S">S.X Fan</name></author><author><name sortKey="Skillman, D R" uniqKey="Skillman D">D.R Skillman</name></author><author><name sortKey="Liao, M J" uniqKey="Liao M">M.J Liao</name></author><author><name sortKey="Testa, D" uniqKey="Testa D">D Testa</name></author><author><name sortKey="Meltzer, M S" uniqKey="Meltzer M">M.S Meltzer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Foster, G R" uniqKey="Foster G">G.R Foster</name></author><author><name sortKey="Rodrigues, O" uniqKey="Rodrigues O">O Rodrigues</name></author><author><name sortKey="Ghouze, F" uniqKey="Ghouze F">F Ghouze</name></author><author><name sortKey="Schulte Frohlinde, E" uniqKey="Schulte Frohlinde E">E Schulte-Frohlinde</name></author><author><name sortKey="Testa, D" uniqKey="Testa D">D Testa</name></author><author><name sortKey="Liao, M J" uniqKey="Liao M">M.J Liao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J Zheng</name></author><author><name sortKey="He, Y Q" uniqKey="He Y">Y.Q He</name></author><author><name sortKey="Liu, X L" uniqKey="Liu X">X.L Liu</name></author><author><name sortKey="Zhuang, Z X" uniqKey="Zhuang Z">Z.X Zhuang</name></author><author><name sortKey="Cheung, C L" uniqKey="Cheung C">C.L Cheung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G Hayden</name></author><author><name sortKey="Gwaltney, J M" uniqKey="Gwaltney J">J.M Gwaltney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Higgins, P G" uniqKey="Higgins P">P.G Higgins</name></author><author><name sortKey="Phillpotts, R J" uniqKey="Phillpotts R">R.J Phillpotts</name></author><author><name sortKey="Scott, G M" uniqKey="Scott G">G.M Scott</name></author><author><name sortKey="Wallace, J" uniqKey="Wallace J">J Wallace</name></author><author><name sortKey="Bernhardt, L L" uniqKey="Bernhardt L">L.L Bernhardt</name></author><author><name sortKey="Tyrrell, D A" uniqKey="Tyrrell D">D.A Tyrrell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Higgins, P G" uniqKey="Higgins P">P.G Higgins</name></author><author><name sortKey="Barrow, G I" uniqKey="Barrow G">G.I Barrow</name></author><author><name sortKey="Tyrrell, D A" uniqKey="Tyrrell D">D.A Tyrrell</name></author><author><name sortKey="Isaacs, D" uniqKey="Isaacs D">D Isaacs</name></author><author><name sortKey="Gauci, C L" uniqKey="Gauci C">C.L Gauci</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karim, A" uniqKey="Karim A">A Karim</name></author><author><name sortKey="Ahmed, S" uniqKey="Ahmed S">S Ahmed</name></author><author><name sortKey="Khan, A" uniqKey="Khan A">A Khan</name></author><author><name sortKey="Steinberg, H" uniqKey="Steinberg H">H Steinberg</name></author><author><name sortKey="Mattana, J" uniqKey="Mattana J">J Mattana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kitamura, K" uniqKey="Kitamura K">K Kitamura</name></author><author><name sortKey="Honda, M" uniqKey="Honda M">M Honda</name></author><author><name sortKey="Yoshizaki, H" uniqKey="Yoshizaki H">H Yoshizaki</name></author><author><name sortKey="Yamamoto, S" uniqKey="Yamamoto S">S Yamamoto</name></author><author><name sortKey="Nakane, H" uniqKey="Nakane H">H Nakane</name></author><author><name sortKey="Fukushima, M" uniqKey="Fukushima M">M Fukushima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lebwohl, M" uniqKey="Lebwohl M">M Lebwohl</name></author><author><name sortKey="Sacks, S" uniqKey="Sacks S">S Sacks</name></author><author><name sortKey="Conant, M" uniqKey="Conant M">M Conant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, B Q" uniqKey="Li B">B.Q Li</name></author><author><name sortKey="Fu, T" uniqKey="Fu T">T Fu</name></author><author><name sortKey="Dongyan, Y" uniqKey="Dongyan Y">Y Dongyan</name></author><author><name sortKey="Mikovits, J A" uniqKey="Mikovits J">J.A Mikovits</name></author><author><name sortKey="Ruscetti, F W" uniqKey="Ruscetti F">F.W Ruscetti</name></author><author><name sortKey="Wang, J M" uniqKey="Wang J">J.M Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, H T" uniqKey="Lu H">H.T Lu</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y Jiang</name></author><author><name sortKey="Chen, F" uniqKey="Chen F">F Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, J D" uniqKey="Meyers J">J.D Meyers</name></author><author><name sortKey="Mcguffin, R W" uniqKey="Mcguffin R">R.W McGuffin</name></author><author><name sortKey="Neiman, P E" uniqKey="Neiman P">P.E Neiman</name></author><author><name sortKey="Singer, J W" uniqKey="Singer J">J.W Singer</name></author><author><name sortKey="Thomas, E D" uniqKey="Thomas E">E.D Thomas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogata, K" uniqKey="Ogata K">K Ogata</name></author><author><name sortKey="Koga, T" uniqKey="Koga T">T Koga</name></author><author><name sortKey="Yagawa, K" uniqKey="Yagawa K">K Yagawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S Peiris</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M Chu</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C Cheng</name></author><author><name sortKey="Chan, K S" uniqKey="Chan K">K.S Chan</name></author><author><name sortKey="Hung, I F" uniqKey="Hung I">I.F Hung</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H Chan</name></author><author><name sortKey="Wong, O K" uniqKey="Wong O">O.K Wong</name></author><author><name sortKey="Yam, W C" uniqKey="Yam W">W.C Yam</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y Yuen</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, R N" uniqKey="Price R">R.N Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raanani, P" uniqKey="Raanani P">P Raanani</name></author><author><name sortKey="Ben Bassat, I" uniqKey="Ben Bassat I">I Ben-Bassat</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Virol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Virol</journal-id><journal-title-group><journal-title>Journal of Clinical Virology</journal-title></journal-title-group><issn pub-type="ppub">1386-6532</issn><issn pub-type="epub">1873-5967</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15288617</article-id><article-id pub-id-type="pmc">7128415</article-id><article-id pub-id-type="publisher-id">S1386-6532(04)00055-1</article-id><article-id pub-id-type="doi">10.1016/j.jcv.2004.03.003</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>In vitro susceptibility of 10 clinical isolates of SARS coronavirus to selected antiviral compounds</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Chen</surname><given-names>F</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>K.H</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Y</given-names></name><xref rid="AFF3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Kao</surname><given-names>R.Y.T</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>H.T</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Fan</surname><given-names>K.W</given-names></name><xref rid="AFF1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Cheng</surname><given-names>V.C.C</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Tsui</surname><given-names>W.H.W</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Hung</surname><given-names>I.F.N</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>T.S.W</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Guan</surname><given-names>Y</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Peiris</surname><given-names>J.S.M</given-names></name><xref rid="AFF2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Yuen</surname><given-names>K.Y</given-names></name><email>kyyuen@hkucc.hku.hk</email><xref rid="AFF2" ref-type="aff">b</xref><xref rid="COR1" ref-type="corresp">∗</xref></contrib></contrib-group><aff id="AFF1"><label>a</label>Centre for Research in Plant Drugs Development, Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong</aff><aff id="AFF2"><label>b</label>Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong</aff><aff id="AFF3"><label>c</label>Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong</aff><author-notes><corresp id="COR1"><label>*</label>Corresponding author. Tel.: +852-28554892; fax: +852-28551241. <email>kyyuen@hkucc.hku.hk</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>18</day><month>5</month><year>2004</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>9</month><year>2004</year></pub-date><pub-date pub-type="epub"><day>18</day><month>5</month><year>2004</year></pub-date><volume>31</volume><issue>1</issue><fpage>69</fpage><lpage>75</lpage><history><date date-type="received"><day>17</day><month>11</month><year>2002</year></date><date date-type="accepted"><day>1</day><month>3</month><year>2004</year></date></history><permissions><copyright-statement>Copyright © 2004 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2004</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>Effective antiviral agents are urgently needed to combat the possible return of severe acute respiratory syndrome (SARS). Commercial antiviral agents and pure chemical compounds extracted from traditional Chinese medicinal herbs were screened against 10 clinical isolates of SARS coronavirus by neutralisation tests with confirmation by plaque reduction assays. Interferon-beta-1a, leukocytic interferon-alpha, ribavirin, lopinavir, rimantadine, baicalin and glycyrrhizin showed antiviral activity. The two interferons were only active if the cell lines were pre-incubated with the drugs 16 h before viral inoculation. Results were confirmed by plaque reduction assays. Antiviral activity varied with the use of different cell lines. Checkerboard assays for synergy were performed showing combinations of interferon beta-1a or leukocytic interferon-alpha with ribavirin are synergistic. Since the clinical and toxicity profiles of these agents are well known, they should be considered either singly or in combination for prophylaxis or treatment of SARS in randomised placebo controlled trials in future epidemics.</p></abstract><kwd-group><title>Keywords</title><kwd>Severe acute respiratory syndrome</kwd><kwd>Antiviral compounds</kwd><kwd>Epidemics</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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