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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine</title><author><name sortKey="Pogrebnyak, Natalia" sort="Pogrebnyak, Natalia" uniqKey="Pogrebnyak N" first="Natalia" last="Pogrebnyak">Natalia Pogrebnyak</name></author><author><name sortKey="Golovkin, Maxim" sort="Golovkin, Maxim" uniqKey="Golovkin M" first="Maxim" last="Golovkin">Maxim Golovkin</name></author><author><name sortKey="Andrianov, Vyacheslav" sort="Andrianov, Vyacheslav" uniqKey="Andrianov V" first="Vyacheslav" last="Andrianov">Vyacheslav Andrianov</name></author><author><name sortKey="Spitsin, Sergei" sort="Spitsin, Sergei" uniqKey="Spitsin S" first="Sergei" last="Spitsin">Sergei Spitsin</name></author><author><name sortKey="Smirnov, Yuriy" sort="Smirnov, Yuriy" uniqKey="Smirnov Y" first="Yuriy" last="Smirnov">Yuriy Smirnov</name></author><author><name sortKey="Egolf, Richard" sort="Egolf, Richard" uniqKey="Egolf R" first="Richard" last="Egolf">Richard Egolf</name></author><author><name sortKey="Koprowski, Hilary" sort="Koprowski, Hilary" uniqKey="Koprowski H" first="Hilary" last="Koprowski">Hilary Koprowski</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15956182</idno><idno type="pmc">1157057</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1157057</idno><idno type="RBID">PMC:1157057</idno><idno type="doi">10.1073/pnas.0503760102</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000745</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000745</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine</title><author><name sortKey="Pogrebnyak, Natalia" sort="Pogrebnyak, Natalia" uniqKey="Pogrebnyak N" first="Natalia" last="Pogrebnyak">Natalia Pogrebnyak</name></author><author><name sortKey="Golovkin, Maxim" sort="Golovkin, Maxim" uniqKey="Golovkin M" first="Maxim" last="Golovkin">Maxim Golovkin</name></author><author><name sortKey="Andrianov, Vyacheslav" sort="Andrianov, Vyacheslav" uniqKey="Andrianov V" first="Vyacheslav" last="Andrianov">Vyacheslav Andrianov</name></author><author><name sortKey="Spitsin, Sergei" sort="Spitsin, Sergei" uniqKey="Spitsin S" first="Sergei" last="Spitsin">Sergei Spitsin</name></author><author><name sortKey="Smirnov, Yuriy" sort="Smirnov, Yuriy" uniqKey="Smirnov Y" first="Yuriy" last="Smirnov">Yuriy Smirnov</name></author><author><name sortKey="Egolf, Richard" sort="Egolf, Richard" uniqKey="Egolf R" first="Richard" last="Egolf">Richard Egolf</name></author><author><name sortKey="Koprowski, Hilary" sort="Koprowski, Hilary" uniqKey="Koprowski H" first="Hilary" last="Koprowski">Hilary Koprowski</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="publisher-id">pnas</journal-id><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15956182</article-id><article-id pub-id-type="pmc">1157057</article-id><article-id pub-id-type="publisher-id">01029062</article-id><article-id pub-id-type="doi">10.1073/pnas.0503760102</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Plant Biology</subject></subj-group></subj-group></article-categories><title-group><article-title>Severe acute respiratory syndrome (SARS) S protein production in plants: Development of recombinant vaccine</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pogrebnyak</surname><given-names>Natalia</given-names></name><xref ref-type="fn" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Golovkin</surname><given-names>Maxim</given-names></name><xref ref-type="fn" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Andrianov</surname><given-names>Vyacheslav</given-names></name></contrib><contrib contrib-type="author"><name><surname>Spitsin</surname><given-names>Sergei</given-names></name></contrib><contrib contrib-type="author"><name><surname>Smirnov</surname><given-names>Yuriy</given-names></name></contrib><contrib contrib-type="author"><name><surname>Egolf</surname><given-names>Richard</given-names></name></contrib><contrib contrib-type="author"><name><surname>Koprowski</surname><given-names>Hilary</given-names></name><xref ref-type="corresp" rid="cor1">†</xref></contrib></contrib-group><aff id="N0x8b5d310.0x8d19740">Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107-6799</aff><author-notes><fn id="cor1"><label>†</label><p> To whom correspondence should be addressed. E-mail: <email>hilary.koprowski@jefferson.edu</email>. </p></fn><fn id="fn1"><label>*</label><p>N.P. and M.G. contributed equally to this work.</p></fn><fn><p>Contributed by Hilary Koprowski, May 10, 2005</p></fn></author-notes><pub-date pub-type="ppub"><day>21</day><month>6</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>14</day><month>6</month><year>2005</year></pub-date><volume>102</volume><issue>25</issue><fpage>9062</fpage><lpage>9067</lpage><copyright-statement>Copyright © 2005, The National Academy of Sciences</copyright-statement><copyright-year>2005</copyright-year><abstract><p>In view of a recent spread of severe acute respiratory syndrome (SARS), there is a high demand for production of a vaccine to prevent this disease. Recent studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments are considered the best candidates for generation of the recombinant vaccine. Toward the development of a safe, effective, and inexpensive vaccine candidate, we have expressed the N-terminal fragment of SARS-CoV S protein (S1) in tomato and low-nicotine tobacco plants. Incorporation of the S1 fragment into plant genomes as well as its transcription was confirmed by PCR and RT-PCR analyses. High levels of expression of recombinant S1 protein were observed in several transgenic lines by Western blot analysis using specific antibodies. Plant-derived antigen was evaluated to induce the systemic and mucosal immune responses in mice. Mice showed significantly increased levels of SARS-CoV-specific IgA after oral ingestion of tomato fruits expressing S1 protein. Sera of mice parenterally primed with tobacco-derived S1 protein revealed the presence of SARS-CoV-specific IgG as detected by Western blot and ELISA analysis.</p></abstract><kwd-group><kwd>immune response</kwd><kwd>plant biotechnology</kwd><kwd>severe acute respiratory syndrome-coronavirus</kwd><kwd>recombinant subunit vaccine</kwd></kwd-group></article-meta><notes><fn-group><fn><p>Abbreviations: SARS, severe acute respiratory syndrome; CoV, coronavirus; S protein, spike protein; SP, signal peptide; g.i., gastric intubation; Km<sup>R</sup>, kanamycin-resistant.</p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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