Identification of the Membrane-Active Regions of the Severe Acute Respiratory Syndrome Coronavirus Spike Membrane Glycoprotein Using a 16/18-Mer Peptide Scan: Implications for the Viral Fusion Mechanism
Identifieur interne : 000688 ( Pmc/Corpus ); précédent : 000687; suivant : 000689Identification of the Membrane-Active Regions of the Severe Acute Respiratory Syndrome Coronavirus Spike Membrane Glycoprotein Using a 16/18-Mer Peptide Scan: Implications for the Viral Fusion Mechanism
Auteurs : Jaime Guillén ; Ana J. Pérez-Berná ; Miguel R. Moreno ; José VillalaínSource :
- Journal of Virology [ 0022-538X ] ; 2005.
Abstract
We have identified the membrane-active regions of the severe acute respiratory syndrome coronavirus (SARS CoV) spike glycoprotein by determining the effect on model membrane integrity of a 16/18-mer SARS CoV spike glycoprotein peptide library. By monitoring the effect of this peptide library on membrane leakage in model membranes, we have identified three regions on the SARS CoV spike glycoprotein with membrane-interacting capabilities: region 1, located immediately upstream of heptad repeat 1 (HR1) and suggested to be the fusion peptide; region 2, located between HR1 and HR2, which would be analogous to the loop domain of human immunodeficiency virus type 1; and region 3, which would correspond to the pretransmembrane region. The identification of these membrane-active regions, which are capable of modifying the biophysical properties of phospholipid membranes, supports their direct role in SARS CoV-mediated membrane fusion, as well as facilitating the future development of SARS CoV entry inhibitors.
Url:
DOI: 10.1128/JVI.79.3.1743-1752.2005
PubMed: 15650199
PubMed Central: 544113
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-id journal-id-type="publisher-id">jvi</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15650199</article-id><article-id pub-id-type="pmc">544113</article-id><article-id pub-id-type="publisher-id">1537-04</article-id><article-id pub-id-type="doi">10.1128/JVI.79.3.1743-1752.2005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Virus-Cell Interactions</subject></subj-group></article-categories><title-group><article-title>Identification of the Membrane-Active Regions of the Severe Acute Respiratory Syndrome Coronavirus Spike Membrane Glycoprotein Using a 16/18-Mer Peptide Scan: Implications for the Viral Fusion Mechanism</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Guillén</surname><given-names>Jaime</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Pérez-Berná</surname><given-names>Ana J.</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Moreno</surname><given-names>Miguel R.</given-names></name><xref ref-type="aff" rid="aff0"></xref></contrib><contrib contrib-type="author"><name><surname>Villalaín</surname><given-names>José</given-names></name><xref ref-type="aff" rid="aff0"></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff0">Instituto de Biología Molecular y Celular, Universidad “Miguel Hernández”, Elche-Alicante, Spain</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Instituto de Biología Molecular y Celular, Universidad “Miguel Hernández”, E-03202 Elche-Alicante, Spain. Phone: 34 966 658 762. Fax: 34 966 658 758. E-mail: <email>jvillalain@umh.es</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>2</month><year>2005</year></pub-date><volume>79</volume><issue>3</issue><fpage>1743</fpage><lpage>1752</lpage><history><date date-type="received"><day>30</day><month>6</month><year>2004</year></date><date date-type="accepted"><day>16</day><month>9</month><year>2004</year></date></history><copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement><copyright-year>2005</copyright-year><abstract><p>We have identified the membrane-active regions of the severe acute respiratory syndrome coronavirus (SARS CoV) spike glycoprotein by determining the effect on model membrane integrity of a 16/18-mer SARS CoV spike glycoprotein peptide library. By monitoring the effect of this peptide library on membrane leakage in model membranes, we have identified three regions on the SARS CoV spike glycoprotein with membrane-interacting capabilities: region 1, located immediately upstream of heptad repeat 1 (HR1) and suggested to be the fusion peptide; region 2, located between HR1 and HR2, which would be analogous to the loop domain of human immunodeficiency virus type 1; and region 3, which would correspond to the pretransmembrane region. The identification of these membrane-active regions, which are capable of modifying the biophysical properties of phospholipid membranes, supports their direct role in SARS CoV-mediated membrane fusion, as well as facilitating the future development of SARS CoV entry inhibitors.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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