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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets</title><author><name sortKey="Hu, Wenqian" sort="Hu, Wenqian" uniqKey="Hu W" first="Wenqian" last="Hu">Wenqian Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Zhihong" sort="Hu, Zhihong" uniqKey="Hu Z" first="Zhihong" last="Hu">Zhihong Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="An, Xuefang" sort="An, Xuefang" uniqKey="An X" first="Xuefang" last="An">Xuefang An</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Lijun" sort="Tang, Lijun" uniqKey="Tang L" first="Lijun" last="Tang">Lijun Tang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hualin" sort="Wang, Hualin" uniqKey="Wang H" first="Hualin" last="Wang">Hualin Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15872219</idno><idno type="pmc">1153763</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153763</idno><idno type="RBID">PMC:1153763</idno><idno type="doi">10.1128/JCM.43.5.2041-2046.2005</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000653</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000653</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets</title><author><name sortKey="Hu, Wenqian" sort="Hu, Wenqian" uniqKey="Hu W" first="Wenqian" last="Hu">Wenqian Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Zhihong" sort="Hu, Zhihong" uniqKey="Hu Z" first="Zhihong" last="Hu">Zhihong Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="An, Xuefang" sort="An, Xuefang" uniqKey="An X" first="Xuefang" last="An">Xuefang An</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tang, Lijun" sort="Tang, Lijun" uniqKey="Tang L" first="Lijun" last="Tang">Lijun Tang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hualin" sort="Wang, Hualin" uniqKey="Wang H" first="Hualin" last="Wang">Hualin Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup> to 10<sup>6</sup> copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-id journal-id-type="publisher-id">jcm</journal-id><journal-title>Journal of Clinical Microbiology</journal-title><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15872219</article-id><article-id pub-id-type="pmc">1153763</article-id><article-id pub-id-type="publisher-id">1736-04</article-id><article-id pub-id-type="doi">10.1128/JCM.43.5.2041-2046.2005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Virology</subject></subj-group></article-categories><title-group><article-title>Development and Evaluation of a Multitarget Real-Time Taqman Reverse Transcription-PCR Assay for Detection of the Severe Acute Respiratory Syndrome-Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Wenqian</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Bai</surname><given-names>Bingke</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Zhihong</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Ze</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>An</surname><given-names>Xuefang</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Lijun</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Jihong</given-names></name><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hualin</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Hanzhong</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1">State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences,<label>1</label> Hubei Center for Diseases Control and Prevention,<label>2</label> Wuhan Center for Diseases Control and Prevention, Wuhan, Hubei, People's Republic of China<label>3</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430072 Hubei, People's Republic of China. Phone: 86-27-87199239. Fax: 86-27-87198072. E-mail: <email>wanghz@pentium.whiov.ac.cn</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>5</month><year>2005</year></pub-date><volume>43</volume><issue>5</issue><fpage>2041</fpage><lpage>2046</lpage><history><date date-type="received"><day>18</day><month>9</month><year>2004</year></date><date date-type="rev-recd"><day>5</day><month>12</month><year>2004</year></date><date date-type="accepted"><day>14</day><month>1</month><year>2005</year></date></history><copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement><copyright-year>2005</copyright-year><abstract><p>Severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is the etiological agent of SARS. It is believed that SARS-CoV originates from wild animals. We have developed a multitarget real-time Taqman reverse transcription-PCR (RT-PCR) assay for the quantitative detection of SARS-CoV. The sequences of the Taqman probes with a minor groove binder and the corresponding primers were based on the sequences of the N gene, open reading frame (ORF) 3, and ORF 8. The overall linear range of this assay was from at least 10<sup>1</sup> to 10<sup>6</sup> copies per reaction, and the detection limit could reach less than 10 copies per reaction. The quantification results for SARS-CoV from cell culture correlated well with those of the RT-PCR by using any two of the three sets of primer and probe used in this assay. However, the results of quantification of SARS-CoV obtained by using a few available throat swab specimens from SARS patients and the N gene as the target were almost 10 times higher than those obtained by using ORF 3 and ORF 8. Using this assay, we also detected an apparently SARS-CoV-related coronavirus in the throat swab specimens from masked palm civets in the west part of Hubei Province, People's Republic of China.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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