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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification</title><author><name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Potter, Simon J" sort="Potter, Simon J" uniqKey="Potter S" first="Simon J." last="Potter">Simon J. Potter</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Lin, Yiguang" sort="Lin, Yiguang" uniqKey="Lin Y" first="Yiguang" last="Lin">Yiguang Lin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L." last="Cunningham">Anthony L. Cunningham</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E." last="Dwyer">Dominic E. Dwyer</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Su, Yuelong" sort="Su, Yuelong" uniqKey="Su Y" first="Yuelong" last="Su">Yuelong Su</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ma, Xuejun" sort="Ma, Xuejun" uniqKey="Ma X" first="Xuejun" last="Ma">Xuejun Ma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hou, Yunde" sort="Hou, Yunde" uniqKey="Hou Y" first="Yunde" last="Hou">Yunde Hou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Saksena, Nitin K" sort="Saksena, Nitin K" uniqKey="Saksena N" first="Nitin K." last="Saksena">Nitin K. Saksena</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15872263</idno><idno type="pmc">1153787</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153787</idno><idno type="RBID">PMC:1153787</idno><idno type="doi">10.1128/JCM.43.5.2339-2344.2005</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000654</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000654</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification</title><author><name sortKey="Wang, Bin" sort="Wang, Bin" uniqKey="Wang B" first="Bin" last="Wang">Bin Wang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Potter, Simon J" sort="Potter, Simon J" uniqKey="Potter S" first="Simon J." last="Potter">Simon J. Potter</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Lin, Yiguang" sort="Lin, Yiguang" uniqKey="Lin Y" first="Yiguang" last="Lin">Yiguang Lin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L." last="Cunningham">Anthony L. Cunningham</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E." last="Dwyer">Dominic E. Dwyer</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Su, Yuelong" sort="Su, Yuelong" uniqKey="Su Y" first="Yuelong" last="Su">Yuelong Su</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Ma, Xuejun" sort="Ma, Xuejun" uniqKey="Ma X" first="Xuejun" last="Ma">Xuejun Ma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hou, Yunde" sort="Hou, Yunde" uniqKey="Hou Y" first="Yunde" last="Hou">Yunde Hou</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Saksena, Nitin K" sort="Saksena, Nitin K" uniqKey="Saksena N" first="Nitin K." last="Saksena">Nitin K. Saksena</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (<italic>n</italic> = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-id journal-id-type="publisher-id">jcm</journal-id><journal-title>Journal of Clinical Microbiology</journal-title><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15872263</article-id><article-id pub-id-type="pmc">1153787</article-id><article-id pub-id-type="publisher-id">1985-04</article-id><article-id pub-id-type="doi">10.1128/JCM.43.5.2339-2344.2005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Virology</subject></subj-group></article-categories><title-group><article-title>Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Bin</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Potter</surname><given-names>Simon J.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Yiguang</given-names></name><xref ref-type="aff" rid="aff1">2</xref></contrib><contrib contrib-type="author"><name><surname>Cunningham</surname><given-names>Anthony L.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Dwyer</surname><given-names>Dominic E.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff1">3</xref></contrib><contrib contrib-type="author"><name><surname>Su</surname><given-names>Yuelong</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Ma</surname><given-names>Xuejun</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Hou</surname><given-names>Yunde</given-names></name><xref ref-type="aff" rid="aff1">4</xref></contrib><contrib contrib-type="author"><name><surname>Saksena</surname><given-names>Nitin K.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1">Centre for Virus Research, Westmead Millennium Institute, The University of Sydney,<label>1</label> Department of Health Sciences, University of Technology,<label>2</label> CIDMLS, ICPMR, Westmead Hospital, Sydney, New South Wales, Australia,<label>3</label> Beijing JDK Bio-technology Institute, National Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China<label>4</label></aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author. Mailing address: Retroviral Genetics Laboratory, Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Darcy Rd., Westmead, Sydney, NSW 2145, Australia. Phone: 61-2-98459107. Fax: 61-2-98459103. E-mail: <email>bin_wang@wmi.usyd.edu.au</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>5</month><year>2005</year></pub-date><volume>43</volume><issue>5</issue><fpage>2339</fpage><lpage>2344</lpage><history><date date-type="received"><day>28</day><month>10</month><year>2004</year></date><date date-type="rev-recd"><day>17</day><month>11</month><year>2004</year></date><date date-type="accepted"><day>5</day><month>1</month><year>2005</year></date></history><copyright-statement>Copyright © 2005, American Society for Microbiology</copyright-statement><copyright-year>2005</copyright-year><abstract><p>The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (<italic>n</italic> = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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