A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Identifieur interne : 001404 ( Pmc/Checkpoint ); précédent : 001403; suivant : 001405A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses
Auteurs : Martin Spruth ; Otfried Kistner ; Helga Savidis-Dacho ; Elisabeth Hitter ; Brian Crowe ; Marijan Gerencer ; Peter Brühl ; Leopold Grillberger ; Manfred Reiter ; Christa Tauer ; Wolfgang Mundt ; P. Noel BarrettSource :
- Vaccine [ 0264-410X ] ; 2005.
Abstract
A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)3 had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.
Url:
DOI: 10.1016/j.vaccine.2005.08.055
PubMed: 16214268
PubMed Central: 7115667
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses</title><author><name sortKey="Spruth, Martin" sort="Spruth, Martin" uniqKey="Spruth M" first="Martin" last="Spruth">Martin Spruth</name></author><author><name sortKey="Kistner, Otfried" sort="Kistner, Otfried" uniqKey="Kistner O" first="Otfried" last="Kistner">Otfried Kistner</name></author><author><name sortKey="Savidis Dacho, Helga" sort="Savidis Dacho, Helga" uniqKey="Savidis Dacho H" first="Helga" last="Savidis-Dacho">Helga Savidis-Dacho</name></author><author><name sortKey="Hitter, Elisabeth" sort="Hitter, Elisabeth" uniqKey="Hitter E" first="Elisabeth" last="Hitter">Elisabeth Hitter</name></author><author><name sortKey="Crowe, Brian" sort="Crowe, Brian" uniqKey="Crowe B" first="Brian" last="Crowe">Brian Crowe</name></author><author><name sortKey="Gerencer, Marijan" sort="Gerencer, Marijan" uniqKey="Gerencer M" first="Marijan" last="Gerencer">Marijan Gerencer</name></author><author><name sortKey="Bruhl, Peter" sort="Bruhl, Peter" uniqKey="Bruhl P" first="Peter" last="Brühl">Peter Brühl</name></author><author><name sortKey="Grillberger, Leopold" sort="Grillberger, Leopold" uniqKey="Grillberger L" first="Leopold" last="Grillberger">Leopold Grillberger</name></author><author><name sortKey="Reiter, Manfred" sort="Reiter, Manfred" uniqKey="Reiter M" first="Manfred" last="Reiter">Manfred Reiter</name></author><author><name sortKey="Tauer, Christa" sort="Tauer, Christa" uniqKey="Tauer C" first="Christa" last="Tauer">Christa Tauer</name></author><author><name sortKey="Mundt, Wolfgang" sort="Mundt, Wolfgang" uniqKey="Mundt W" first="Wolfgang" last="Mundt">Wolfgang Mundt</name></author><author><name sortKey="Barrett, P Noel" sort="Barrett, P Noel" uniqKey="Barrett P" first="P. 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Noel Barrett</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16214268</idno><idno type="pmc">7115667</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115667</idno><idno type="RBID">PMC:7115667</idno><idno type="doi">10.1016/j.vaccine.2005.08.055</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">001526</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001526</idno><idno type="wicri:Area/Pmc/Curation">001526</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001526</idno><idno type="wicri:Area/Pmc/Checkpoint">001404</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001404</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses</title><author><name sortKey="Spruth, Martin" sort="Spruth, Martin" uniqKey="Spruth M" first="Martin" last="Spruth">Martin Spruth</name></author><author><name sortKey="Kistner, Otfried" sort="Kistner, Otfried" uniqKey="Kistner O" first="Otfried" last="Kistner">Otfried Kistner</name></author><author><name sortKey="Savidis Dacho, Helga" sort="Savidis Dacho, Helga" uniqKey="Savidis Dacho H" first="Helga" last="Savidis-Dacho">Helga Savidis-Dacho</name></author><author><name sortKey="Hitter, Elisabeth" sort="Hitter, Elisabeth" uniqKey="Hitter E" first="Elisabeth" last="Hitter">Elisabeth Hitter</name></author><author><name sortKey="Crowe, Brian" sort="Crowe, Brian" uniqKey="Crowe B" first="Brian" last="Crowe">Brian Crowe</name></author><author><name sortKey="Gerencer, Marijan" sort="Gerencer, Marijan" uniqKey="Gerencer M" first="Marijan" last="Gerencer">Marijan Gerencer</name></author><author><name sortKey="Bruhl, Peter" sort="Bruhl, Peter" uniqKey="Bruhl P" first="Peter" last="Brühl">Peter Brühl</name></author><author><name sortKey="Grillberger, Leopold" sort="Grillberger, Leopold" uniqKey="Grillberger L" first="Leopold" last="Grillberger">Leopold Grillberger</name></author><author><name sortKey="Reiter, Manfred" sort="Reiter, Manfred" uniqKey="Reiter M" first="Manfred" last="Reiter">Manfred Reiter</name></author><author><name sortKey="Tauer, Christa" sort="Tauer, Christa" uniqKey="Tauer C" first="Christa" last="Tauer">Christa Tauer</name></author><author><name sortKey="Mundt, Wolfgang" sort="Mundt, Wolfgang" uniqKey="Mundt W" first="Wolfgang" last="Mundt">Wolfgang Mundt</name></author><author><name sortKey="Barrett, P Noel" sort="Barrett, P Noel" uniqKey="Barrett P" first="P. Noel" last="Barrett">P. Noel Barrett</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)<sub>3</sub> had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, J S M" uniqKey="Peiris J">J.S.M. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S.T. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id><journal-id journal-id-type="iso-abbrev">Vaccine</journal-id><journal-title-group><journal-title>Vaccine</journal-title></journal-title-group><issn pub-type="ppub">0264-410X</issn><issn pub-type="epub">1873-2518</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16214268</article-id><article-id pub-id-type="pmc">7115667</article-id><article-id pub-id-type="publisher-id">S0264-410X(05)00867-4</article-id><article-id pub-id-type="doi">10.1016/j.vaccine.2005.08.055</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A double-inactivated whole virus candidate SARS coronavirus vaccine stimulates neutralising and protective antibody responses</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Spruth</surname><given-names>Martin</given-names></name><email>martin_spruth@baxter.com</email><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author"><name><surname>Kistner</surname><given-names>Otfried</given-names></name></contrib><contrib contrib-type="author"><name><surname>Savidis-Dacho</surname><given-names>Helga</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hitter</surname><given-names>Elisabeth</given-names></name></contrib><contrib contrib-type="author"><name><surname>Crowe</surname><given-names>Brian</given-names></name></contrib><contrib contrib-type="author"><name><surname>Gerencer</surname><given-names>Marijan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Brühl</surname><given-names>Peter</given-names></name></contrib><contrib contrib-type="author"><name><surname>Grillberger</surname><given-names>Leopold</given-names></name></contrib><contrib contrib-type="author"><name><surname>Reiter</surname><given-names>Manfred</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tauer</surname><given-names>Christa</given-names></name></contrib><contrib contrib-type="author"><name><surname>Mundt</surname><given-names>Wolfgang</given-names></name></contrib><contrib contrib-type="author"><name><surname>Barrett</surname><given-names>P. Noel</given-names></name></contrib></contrib-group><aff>Baxter Vaccines, Biomedical Research Centre, Uferstr. 15, 2304 Orth/Donau, Austria</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author. Tel.: +43 1 20100 4310; fax: +43 1 20100 4000. <email>martin_spruth@baxter.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>26</day><month>8</month><year>2005</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>30</day><month>1</month><year>2006</year></pub-date><pub-date pub-type="epub"><day>26</day><month>8</month><year>2005</year></pub-date><volume>24</volume><issue>5</issue><fpage>652</fpage><lpage>661</lpage><history><date date-type="received"><day>31</day><month>5</month><year>2005</year></date><date date-type="accepted"><day>5</day><month>8</month><year>2005</year></date></history><permissions><copyright-statement>Copyright © 2005 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2005</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>A double-inactivated, candidate whole virus vaccine against severe acute respiratory syndrome associated coronavirus (SARS-CoV) was developed and manufactured at large scale using fermenter cultures of serum protein free Vero cells. A two step inactivation procedure involving sequential formaldehyde and U.V. inactivation was utilised in order to ensure an extremely high safety margin with respect to residual infectivity. The immunogenicity of this double-inactivated vaccine was characterised in the mouse model. Mice that were immunised twice with the candidate SARS-CoV vaccine developed high antibody titres against the SARS-CoV spike protein and high levels of neutralising antibodies. The use of the adjuvant Al(OH)<sub>3</sub> had only a minor effect on the immunogenicity of the vaccine. In addition, cell mediated immunity as measured by interferon-γ and interleukin-4 stimulation, was elicited by vaccination. Moreover, the vaccine confers protective immunity as demonstrated by prevention of SARS-CoV replication in the respiratory tract of mice after intranasal challenge with SARS-CoV. Protection of mice was correlated to antibody titre against the SARS-CoV S protein and neutralising antibody titre.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS coronavirus</kwd><kwd>Double-inactivated vaccine</kwd><kwd>Neutralisation</kwd><kwd>Protection</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Barrett, P Noel" sort="Barrett, P Noel" uniqKey="Barrett P" first="P. Noel" last="Barrett">P. Noel Barrett</name><name sortKey="Bruhl, Peter" sort="Bruhl, Peter" uniqKey="Bruhl P" first="Peter" last="Brühl">Peter Brühl</name><name sortKey="Crowe, Brian" sort="Crowe, Brian" uniqKey="Crowe B" first="Brian" last="Crowe">Brian Crowe</name><name sortKey="Gerencer, Marijan" sort="Gerencer, Marijan" uniqKey="Gerencer M" first="Marijan" last="Gerencer">Marijan Gerencer</name><name sortKey="Grillberger, Leopold" sort="Grillberger, Leopold" uniqKey="Grillberger L" first="Leopold" last="Grillberger">Leopold Grillberger</name><name sortKey="Hitter, Elisabeth" sort="Hitter, Elisabeth" uniqKey="Hitter E" first="Elisabeth" last="Hitter">Elisabeth Hitter</name><name sortKey="Kistner, Otfried" sort="Kistner, Otfried" uniqKey="Kistner O" first="Otfried" last="Kistner">Otfried Kistner</name><name sortKey="Mundt, Wolfgang" sort="Mundt, Wolfgang" uniqKey="Mundt W" first="Wolfgang" last="Mundt">Wolfgang Mundt</name><name sortKey="Reiter, Manfred" sort="Reiter, Manfred" uniqKey="Reiter M" first="Manfred" last="Reiter">Manfred Reiter</name><name sortKey="Savidis Dacho, Helga" sort="Savidis Dacho, Helga" uniqKey="Savidis Dacho H" first="Helga" last="Savidis-Dacho">Helga Savidis-Dacho</name><name sortKey="Spruth, Martin" sort="Spruth, Martin" uniqKey="Spruth M" first="Martin" last="Spruth">Martin Spruth</name><name sortKey="Tauer, Christa" sort="Tauer, Christa" uniqKey="Tauer C" first="Christa" last="Tauer">Christa Tauer</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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