Molecular cloning, expression, and purification of SARS-CoV nsp13
Identifieur interne : 001296 ( Pmc/Checkpoint ); précédent : 001295; suivant : 001297Molecular cloning, expression, and purification of SARS-CoV nsp13
Auteurs : Zheng Fan [République populaire de Chine] ; Kunpeng Peng [République populaire de Chine] ; Xinyu Tan [République populaire de Chine] ; Bin Yin [République populaire de Chine] ; Xiuhua Dong [République populaire de Chine] ; Feichan Qiu [République populaire de Chine] ; Yan Shen [République populaire de Chine] ; Heng Wang [République populaire de Chine] ; Jiangang Yuan [République populaire de Chine] ; Boqin Qiang [République populaire de Chine] ; Xiaozhong Peng [République populaire de Chine]Source :
- Protein Expression and Purification [ 1046-5928 ] ; 2005.
Abstract
The SARS-nsp13 protein was identified as an mRNA cap1 methyltransferase. In this study, the nsp13 gene was cloned from the SARS-CoV PUMC02 strain viral RNA by RT-PCR, and inserted into the expression plasmid pET30a(+). The recombinant plasmid pET30a(+)-nsp13 was confirmed by restriction enzymes and sequencing analysis, and transformed into
Url:
DOI: 10.1016/j.pep.2004.08.003
PubMed: 15866708
PubMed Central: 7129550
Affiliations:
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<author><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name>
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<author><name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
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<author><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
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<author><name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name>
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<author><name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
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<author><name sortKey="Qiang, Boqin" sort="Qiang, Boqin" uniqKey="Qiang B" first="Boqin" last="Qiang">Boqin Qiang</name>
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<placeName><settlement type="city">Pékin</settlement>
</placeName>
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<author><name sortKey="Peng, Xiaozhong" sort="Peng, Xiaozhong" uniqKey="Peng X" first="Xiaozhong" last="Peng">Xiaozhong Peng</name>
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<series><title level="j">Protein Expression and Purification</title>
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<front><div type="abstract" xml:lang="en"><p>The SARS-nsp13 protein was identified as an mRNA cap1 methyltransferase. In this study, the nsp13 gene was cloned from the SARS-CoV PUMC02 strain viral RNA by RT-PCR, and inserted into the expression plasmid pET30a(+). The recombinant plasmid pET30a(+)-nsp13 was confirmed by restriction enzymes and sequencing analysis, and transformed into <italic>Escherichia coli</italic>
BL21(DE3). The His-tag-fused protein was expressed by induction of 0.5 mM IPTG and purified by a single Ni<sup>2+</sup>
affinity chromatography. The protein was validated by western blot and MS analysis. A large quantity of the nsp13 protein obtained with this method may be useful for further study of its structure and function.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name>
</author>
<author><name sortKey="Erdman, D" uniqKey="Erdman D">D. Erdman</name>
</author>
<author><name sortKey="Goldsmith, C S" uniqKey="Goldsmith C">C.S. Goldsmith</name>
</author>
<author><name sortKey="Zaki, S R" uniqKey="Zaki S">S.R. Zaki</name>
</author>
<author><name sortKey="Peret, T" uniqKey="Peret T">T. Peret</name>
</author>
<author><name sortKey="Emery, S" uniqKey="Emery S">S. Emery</name>
</author>
<author><name sortKey="Tong, S" uniqKey="Tong S">S. Tong</name>
</author>
<author><name sortKey="Urbani, C" uniqKey="Urbani C">C. Urbani</name>
</author>
<author><name sortKey="Comer, J A" uniqKey="Comer J">J.A. Comer</name>
</author>
<author><name sortKey="Lim, W" uniqKey="Lim W">W. Lim</name>
</author>
<author><name sortKey="Rollin, P E" uniqKey="Rollin P">P.E. Rollin</name>
</author>
<author><name sortKey="Dowell, S F" uniqKey="Dowell S">S.F. Dowell</name>
</author>
<author><name sortKey="Ling, A E" uniqKey="Ling A">A.E. Ling</name>
</author>
<author><name sortKey="Humphrey, C D" uniqKey="Humphrey C">C.D. Humphrey</name>
</author>
<author><name sortKey="Shieh, W J" uniqKey="Shieh W">W.J. Shieh</name>
</author>
<author><name sortKey="Guarner, J" uniqKey="Guarner J">J. Guarner</name>
</author>
<author><name sortKey="Paddock, C D" uniqKey="Paddock C">C.D. Paddock</name>
</author>
<author><name sortKey="Rota, P" uniqKey="Rota P">P. Rota</name>
</author>
<author><name sortKey="Fields, B" uniqKey="Fields B">B. Fields</name>
</author>
<author><name sortKey="Derisi, J" uniqKey="Derisi J">J. DeRisi</name>
</author>
<author><name sortKey="Yang, J Y" uniqKey="Yang J">J.Y. Yang</name>
</author>
<author><name sortKey="Cox, N" uniqKey="Cox N">N. Cox</name>
</author>
<author><name sortKey="Hughes, J M" uniqKey="Hughes J">J.M. Hughes</name>
</author>
<author><name sortKey="Leduc, J W" uniqKey="Leduc J">J.W. LeDuc</name>
</author>
<author><name sortKey="Bellini, W J" uniqKey="Bellini W">W.J. Bellini</name>
</author>
<author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name>
</author>
<author><name sortKey="Vander Werf, S" uniqKey="Vander Werf S">S. vander Werf</name>
</author>
<author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. Brodt</name>
</author>
<author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name>
</author>
<author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H. Rabenau</name>
</author>
<author><name sortKey="Panning, M" uniqKey="Panning M">M. Panning</name>
</author>
<author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L. Kolesnikova</name>
</author>
<author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name>
</author>
<author><name sortKey="Berger, A" uniqKey="Berger A">A. Berger</name>
</author>
<author><name sortKey="Burguiere, A M" uniqKey="Burguiere A">A.M. Burguiere</name>
</author>
<author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J. Cinatl</name>
</author>
<author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M. Eickmann</name>
</author>
<author><name sortKey="Escriou, N" uniqKey="Escriou N">N. Escriou</name>
</author>
<author><name sortKey="Grywna, K" uniqKey="Grywna K">K. Grywna</name>
</author>
<author><name sortKey="Kramme, S" uniqKey="Kramme S">S. Kramme</name>
</author>
<author><name sortKey="Manuguerra, J C" uniqKey="Manuguerra J">J.C. Manuguerra</name>
</author>
<author><name sortKey="Muller, S" uniqKey="Muller S">S. Muller</name>
</author>
<author><name sortKey="Rickerts, V" uniqKey="Rickerts V">V. Rickerts</name>
</author>
<author><name sortKey="Sturmer, M" uniqKey="Sturmer M">M. Sturmer</name>
</author>
<author><name sortKey="Vieth, S" uniqKey="Vieth S">S. Vieth</name>
</author>
<author><name sortKey="Klenk, H D" uniqKey="Klenk H">H.D. Klenk</name>
</author>
<author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name>
</author>
<author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H. Schmitz</name>
</author>
<author><name sortKey="Doerr, H W" uniqKey="Doerr H">H.W. Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poutanen, S M" uniqKey="Poutanen S">S.M. Poutanen</name>
</author>
<author><name sortKey="Low, D E" uniqKey="Low D">D.E. Low</name>
</author>
<author><name sortKey="Henry, B" uniqKey="Henry B">B. Henry</name>
</author>
<author><name sortKey="Finkelstein, S" uniqKey="Finkelstein S">S. Finkelstein</name>
</author>
<author><name sortKey="Rose, D" uniqKey="Rose D">D. Rose</name>
</author>
<author><name sortKey="Green, K" uniqKey="Green K">K. Green</name>
</author>
<author><name sortKey="Tellier, R" uniqKey="Tellier R">R. Tellier</name>
</author>
<author><name sortKey="Draker, R" uniqKey="Draker R">R. Draker</name>
</author>
<author><name sortKey="Adachi, D" uniqKey="Adachi D">D. Adachi</name>
</author>
<author><name sortKey="Ayers, M" uniqKey="Ayers M">M. Ayers</name>
</author>
<author><name sortKey="Chan, A K" uniqKey="Chan A">A.K. Chan</name>
</author>
<author><name sortKey="Skowronski, D M" uniqKey="Skowronski D">D.M. Skowronski</name>
</author>
<author><name sortKey="Salit, I" uniqKey="Salit I">I. Salit</name>
</author>
<author><name sortKey="Simor, A E" uniqKey="Simor A">A.E. Simor</name>
</author>
<author><name sortKey="Slutsky, A S" uniqKey="Slutsky A">A.S. Slutsky</name>
</author>
<author><name sortKey="Doyle, P W" uniqKey="Doyle P">P.W. Doyle</name>
</author>
<author><name sortKey="Krajden, M" uniqKey="Krajden M">M. Krajden</name>
</author>
<author><name sortKey="Petric, M" uniqKey="Petric M">M. Petric</name>
</author>
<author><name sortKey="Brunham, R C" uniqKey="Brunham R">R.C. Brunham</name>
</author>
<author><name sortKey="Mcgeer, A J" uniqKey="Mcgeer A">A.J. McGeer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name>
</author>
<author><name sortKey="Oberste, M S" uniqKey="Oberste M">M.S. Oberste</name>
</author>
<author><name sortKey="Monroe, S S" uniqKey="Monroe S">S.S. Monroe</name>
</author>
<author><name sortKey="Nix, W A" uniqKey="Nix W">W.A. Nix</name>
</author>
<author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R. Campagnoli</name>
</author>
<author><name sortKey="Icenogle, J P" uniqKey="Icenogle J">J.P. Icenogle</name>
</author>
<author><name sortKey="Penaranda, S" uniqKey="Penaranda S">S. Penaranda</name>
</author>
<author><name sortKey="Bankamp, B" uniqKey="Bankamp B">B. Bankamp</name>
</author>
<author><name sortKey="Maher, K" uniqKey="Maher K">K. Maher</name>
</author>
<author><name sortKey="Chen, M H" uniqKey="Chen M">M.H. Chen</name>
</author>
<author><name sortKey="Tong, S" uniqKey="Tong S">S. Tong</name>
</author>
<author><name sortKey="Tamin, A" uniqKey="Tamin A">A. Tamin</name>
</author>
<author><name sortKey="Lowe, L" uniqKey="Lowe L">L. Lowe</name>
</author>
<author><name sortKey="Frace, M" uniqKey="Frace M">M. Frace</name>
</author>
<author><name sortKey="Derisi, J L" uniqKey="Derisi J">J.L. DeRisi</name>
</author>
<author><name sortKey="Chen, Q" uniqKey="Chen Q">Q. Chen</name>
</author>
<author><name sortKey="Wang, D" uniqKey="Wang D">D. Wang</name>
</author>
<author><name sortKey="Erdman, D D" uniqKey="Erdman D">D.D. Erdman</name>
</author>
<author><name sortKey="Peret, T C" uniqKey="Peret T">T.C. Peret</name>
</author>
<author><name sortKey="Burns, C" uniqKey="Burns C">C. Burns</name>
</author>
<author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name>
</author>
<author><name sortKey="Rollin, P E" uniqKey="Rollin P">P.E. Rollin</name>
</author>
<author><name sortKey="Sanchez, A" uniqKey="Sanchez A">A. Sanchez</name>
</author>
<author><name sortKey="Liffick, S" uniqKey="Liffick S">S. Liffick</name>
</author>
<author><name sortKey="Holloway, B" uniqKey="Holloway B">B. Holloway</name>
</author>
<author><name sortKey="Limor, J" uniqKey="Limor J">J. Limor</name>
</author>
<author><name sortKey="Mccaustland, K" uniqKey="Mccaustland K">K. McCaustland</name>
</author>
<author><name sortKey="Olsen Rasmussen, M" uniqKey="Olsen Rasmussen M">M. Olsen-Rasmussen</name>
</author>
<author><name sortKey="Fouchier, R" uniqKey="Fouchier R">R. Fouchier</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name>
</author>
<author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name>
</author>
<author><name sortKey="Pallansch, M A" uniqKey="Pallansch M">M.A. Pallansch</name>
</author>
<author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name>
</author>
<author><name sortKey="Bellini, W J" uniqKey="Bellini W">W.J. Bellini</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name>
</author>
<author><name sortKey="Jones, S J" uniqKey="Jones S">S.J. Jones</name>
</author>
<author><name sortKey="Astell, C R" uniqKey="Astell C">C.R. Astell</name>
</author>
<author><name sortKey="Holt, R A" uniqKey="Holt R">R.A. Holt</name>
</author>
<author><name sortKey="Brooks Wilson, A" uniqKey="Brooks Wilson A">A. Brooks-Wilson</name>
</author>
<author><name sortKey="Butterfield, Y S" uniqKey="Butterfield Y">Y.S. Butterfield</name>
</author>
<author><name sortKey="Khattra, J" uniqKey="Khattra J">J. Khattra</name>
</author>
<author><name sortKey="Asano, J K" uniqKey="Asano J">J.K. Asano</name>
</author>
<author><name sortKey="Barber, S A" uniqKey="Barber S">S.A. Barber</name>
</author>
<author><name sortKey="Chan, S Y" uniqKey="Chan S">S.Y. Chan</name>
</author>
<author><name sortKey="Cloutier, A" uniqKey="Cloutier A">A. Cloutier</name>
</author>
<author><name sortKey="Coughlin, S M" uniqKey="Coughlin S">S.M. Coughlin</name>
</author>
<author><name sortKey="Freeman, D" uniqKey="Freeman D">D. Freeman</name>
</author>
<author><name sortKey="Girn, N" uniqKey="Girn N">N. Girn</name>
</author>
<author><name sortKey="Griffith, O L" uniqKey="Griffith O">O.L. Griffith</name>
</author>
<author><name sortKey="Leach, S R" uniqKey="Leach S">S.R. Leach</name>
</author>
<author><name sortKey="Mayo, M" uniqKey="Mayo M">M. Mayo</name>
</author>
<author><name sortKey="Mcdonald, H" uniqKey="Mcdonald H">H. McDonald</name>
</author>
<author><name sortKey="Montgomery, S B" uniqKey="Montgomery S">S.B. Montgomery</name>
</author>
<author><name sortKey="Pandoh, P K" uniqKey="Pandoh P">P.K. Pandoh</name>
</author>
<author><name sortKey="Petrescu, A S" uniqKey="Petrescu A">A.S. Petrescu</name>
</author>
<author><name sortKey="Robertson, A G" uniqKey="Robertson A">A.G. Robertson</name>
</author>
<author><name sortKey="Schein, J E" uniqKey="Schein J">J.E. Schein</name>
</author>
<author><name sortKey="Siddiqui, A" uniqKey="Siddiqui A">A. Siddiqui</name>
</author>
<author><name sortKey="Smailus, D E" uniqKey="Smailus D">D.E. Smailus</name>
</author>
<author><name sortKey="Stott, J M" uniqKey="Stott J">J.M. Stott</name>
</author>
<author><name sortKey="Yang, G S" uniqKey="Yang G">G.S. Yang</name>
</author>
<author><name sortKey="Plummer, F" uniqKey="Plummer F">F. Plummer</name>
</author>
<author><name sortKey="Andonov, A" uniqKey="Andonov A">A. Andonov</name>
</author>
<author><name sortKey="Artsob, H" uniqKey="Artsob H">H. Artsob</name>
</author>
<author><name sortKey="Bastien, N" uniqKey="Bastien N">N. Bastien</name>
</author>
<author><name sortKey="Bernard, K" uniqKey="Bernard K">K. Bernard</name>
</author>
<author><name sortKey="Booth, T F" uniqKey="Booth T">T.F. Booth</name>
</author>
<author><name sortKey="Bowness, D" uniqKey="Bowness D">D. Bowness</name>
</author>
<author><name sortKey="Czub, M" uniqKey="Czub M">M. Czub</name>
</author>
<author><name sortKey="Drebot, M" uniqKey="Drebot M">M. Drebot</name>
</author>
<author><name sortKey="Fernando, L" uniqKey="Fernando L">L. Fernando</name>
</author>
<author><name sortKey="Flick, R" uniqKey="Flick R">R. Flick</name>
</author>
<author><name sortKey="Garbutt, M" uniqKey="Garbutt M">M. Garbutt</name>
</author>
<author><name sortKey="Gray, M" uniqKey="Gray M">M. Gray</name>
</author>
<author><name sortKey="Grolla, A" uniqKey="Grolla A">A. Grolla</name>
</author>
<author><name sortKey="Jones, S" uniqKey="Jones S">S. Jones</name>
</author>
<author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name>
</author>
<author><name sortKey="Meyers, A" uniqKey="Meyers A">A. Meyers</name>
</author>
<author><name sortKey="Kabani, A" uniqKey="Kabani A">A. Kabani</name>
</author>
<author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name>
</author>
<author><name sortKey="Normand, S" uniqKey="Normand S">S. Normand</name>
</author>
<author><name sortKey="Stroher, U" uniqKey="Stroher U">U. Stroher</name>
</author>
<author><name sortKey="Tipples, G A" uniqKey="Tipples G">G.A. Tipples</name>
</author>
<author><name sortKey="Tyler, S" uniqKey="Tyler S">S. Tyler</name>
</author>
<author><name sortKey="Vogrig, R" uniqKey="Vogrig R">R. Vogrig</name>
</author>
<author><name sortKey="Ward, D" uniqKey="Ward D">D. Ward</name>
</author>
<author><name sortKey="Watson, B" uniqKey="Watson B">B. Watson</name>
</author>
<author><name sortKey="Brunham, R C" uniqKey="Brunham R">R.C. Brunham</name>
</author>
<author><name sortKey="Krajden, M" uniqKey="Krajden M">M. Krajden</name>
</author>
<author><name sortKey="Petric, M" uniqKey="Petric M">M. Petric</name>
</author>
<author><name sortKey="Skowronski, D M" uniqKey="Skowronski D">D.M. Skowronski</name>
</author>
<author><name sortKey="Upton, C" uniqKey="Upton C">C. Upton</name>
</author>
<author><name sortKey="Roper, R L" uniqKey="Roper R">R.L. Roper</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Von Grotthuss, M" uniqKey="Von Grotthuss M">M. von Grotthuss</name>
</author>
<author><name sortKey="Wyrwicz, L S" uniqKey="Wyrwicz L">L.S. Wyrwicz</name>
</author>
<author><name sortKey="Rychlewski, L" uniqKey="Rychlewski L">L. Rychlewski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shuman, S" uniqKey="Shuman S">S. Shuman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bisaillon, M" uniqKey="Bisaillon M">M. Bisaillon</name>
</author>
<author><name sortKey="Lemay, G" uniqKey="Lemay G">G. Lemay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furuichi, Y" uniqKey="Furuichi Y">Y. Furuichi</name>
</author>
<author><name sortKey="Shatkin, A J" uniqKey="Shatkin A">A.J. Shatkin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Testa, D" uniqKey="Testa D">D. Testa</name>
</author>
<author><name sortKey="Banerjee, A K" uniqKey="Banerjee A">A.K. Banerjee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hammond, D C" uniqKey="Hammond D">D.C. Hammond</name>
</author>
<author><name sortKey="Lesnaw, J A" uniqKey="Lesnaw J">J.A. Lesnaw</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J. Sambrook</name>
</author>
<author><name sortKey="Russell, D W" uniqKey="Russell D">D.W. Russell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shevchenko, A" uniqKey="Shevchenko A">A. Shevchenko</name>
</author>
<author><name sortKey="Wilm, M" uniqKey="Wilm M">M. Wilm</name>
</author>
<author><name sortKey="Vorm, O" uniqKey="Vorm O">O. Vorm</name>
</author>
<author><name sortKey="Mann, M" uniqKey="Mann M">M. Mann</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Protein Expr Purif</journal-id>
<journal-id journal-id-type="iso-abbrev">Protein Expr. Purif</journal-id>
<journal-title-group><journal-title>Protein Expression and Purification</journal-title>
</journal-title-group>
<issn pub-type="ppub">1046-5928</issn>
<issn pub-type="epub">1096-0279</issn>
<publisher><publisher-name>Elsevier Inc.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">15866708</article-id>
<article-id pub-id-type="pmc">7129550</article-id>
<article-id pub-id-type="publisher-id">S1046-5928(04)00278-5</article-id>
<article-id pub-id-type="doi">10.1016/j.pep.2004.08.003</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Molecular cloning, expression, and purification of SARS-CoV nsp13</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Fan</surname>
<given-names>Zheng</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="fn1" ref-type="fn">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Peng</surname>
<given-names>Kunpeng</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="fn1" ref-type="fn">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tan</surname>
<given-names>Xinyu</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yin</surname>
<given-names>Bin</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Dong</surname>
<given-names>Xiuhua</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qiu</surname>
<given-names>Feichan</given-names>
</name>
<xref rid="aff2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shen</surname>
<given-names>Yan</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Heng</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yuan</surname>
<given-names>Jiangang</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qiang</surname>
<given-names>Boqin</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Peng</surname>
<given-names>Xiaozhong</given-names>
</name>
<email>pengxiaozhong@pumc.edu.cn</email>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, National Human Genome Center, Beijing 100005, China</aff>
<aff id="aff2"><label>b</label>
Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author. Fax: +86 10 65240529 <email>pengxiaozhong@pumc.edu.cn</email>
</corresp>
<fn id="fn1"><label>1</label>
<p>These two authors contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="pmc-release"><day>11</day>
<month>4</month>
<year>2005</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>6</month>
<year>2005</year>
</pub-date>
<pub-date pub-type="epub"><day>11</day>
<month>4</month>
<year>2005</year>
</pub-date>
<volume>41</volume>
<issue>2</issue>
<fpage>235</fpage>
<lpage>240</lpage>
<history><date date-type="received"><day>28</day>
<month>4</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>29</day>
<month>7</month>
<year>2004</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2005 Elsevier Inc. All rights reserved.</copyright-statement>
<copyright-year>2005</copyright-year>
<copyright-holder>Elsevier Inc.</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract><p>The SARS-nsp13 protein was identified as an mRNA cap1 methyltransferase. In this study, the nsp13 gene was cloned from the SARS-CoV PUMC02 strain viral RNA by RT-PCR, and inserted into the expression plasmid pET30a(+). The recombinant plasmid pET30a(+)-nsp13 was confirmed by restriction enzymes and sequencing analysis, and transformed into <italic>Escherichia coli</italic>
BL21(DE3). The His-tag-fused protein was expressed by induction of 0.5 mM IPTG and purified by a single Ni<sup>2+</sup>
affinity chromatography. The protein was validated by western blot and MS analysis. A large quantity of the nsp13 protein obtained with this method may be useful for further study of its structure and function.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd>Severe acute respiratory syndrome (SARS)</kwd>
<kwd>nsp13 (SARS-CoV methyltransferase)</kwd>
<kwd>Molecular cloning</kwd>
<kwd>Expression</kwd>
<kwd>Purification</kwd>
<kwd>Mass spectrometric identification</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name>
</noRegion>
<name sortKey="Dong, Xiuhua" sort="Dong, Xiuhua" uniqKey="Dong X" first="Xiuhua" last="Dong">Xiuhua Dong</name>
<name sortKey="Peng, Kunpeng" sort="Peng, Kunpeng" uniqKey="Peng K" first="Kunpeng" last="Peng">Kunpeng Peng</name>
<name sortKey="Peng, Xiaozhong" sort="Peng, Xiaozhong" uniqKey="Peng X" first="Xiaozhong" last="Peng">Xiaozhong Peng</name>
<name sortKey="Qiang, Boqin" sort="Qiang, Boqin" uniqKey="Qiang B" first="Boqin" last="Qiang">Boqin Qiang</name>
<name sortKey="Qiu, Feichan" sort="Qiu, Feichan" uniqKey="Qiu F" first="Feichan" last="Qiu">Feichan Qiu</name>
<name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
<name sortKey="Tan, Xinyu" sort="Tan, Xinyu" uniqKey="Tan X" first="Xinyu" last="Tan">Xinyu Tan</name>
<name sortKey="Wang, Heng" sort="Wang, Heng" uniqKey="Wang H" first="Heng" last="Wang">Heng Wang</name>
<name sortKey="Yin, Bin" sort="Yin, Bin" uniqKey="Yin B" first="Bin" last="Yin">Bin Yin</name>
<name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
</country>
</tree>
</affiliations>
</record>
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