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Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells

Identifieur interne : 001088 ( Pmc/Checkpoint ); précédent : 001087; suivant : 001089

Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells

Auteurs : Sehaam Khan ; Burtram C. Fielding ; Timothy H. P. Tan ; Chih-Fong Chou ; Shuo Shen ; Seng Gee Lim ; Wanjin Hong ; Yee-Joo Tan

Source :

RBID : PMC:7114230

Abstract

The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bΔ124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bΔ124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.


Url:
DOI: 10.1016/j.virusres.2006.06.005
PubMed: 16965829
PubMed Central: 7114230


Affiliations:


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PMC:7114230

Le document en format XML

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<p>The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bΔ124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bΔ124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.</p>
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</TEI>
<pmc article-type="research-article">
<pmc-dir>properties open_access</pmc-dir>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Virus Res</journal-id>
<journal-id journal-id-type="iso-abbrev">Virus Res</journal-id>
<journal-title-group>
<journal-title>Virus Research</journal-title>
</journal-title-group>
<issn pub-type="ppub">0168-1702</issn>
<issn pub-type="epub">1872-7492</issn>
<publisher>
<publisher-name>Elsevier B.V.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="pmid">16965829</article-id>
<article-id pub-id-type="pmc">7114230</article-id>
<article-id pub-id-type="publisher-id">S0168-1702(06)00191-2</article-id>
<article-id pub-id-type="doi">10.1016/j.virusres.2006.06.005</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Article</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Khan</surname>
<given-names>Sehaam</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Fielding</surname>
<given-names>Burtram C.</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Tan</surname>
<given-names>Timothy H.P.</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Chou</surname>
<given-names>Chih-Fong</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Shen</surname>
<given-names>Shuo</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Lim</surname>
<given-names>Seng Gee</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Hong</surname>
<given-names>Wanjin</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Tan</surname>
<given-names>Yee-Joo</given-names>
</name>
<email>mcbtanyj@imcb.a-star.edu.sg</email>
<xref rid="cor1" ref-type="corresp"></xref>
</contrib>
</contrib-group>
<aff>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore</aff>
<author-notes>
<corresp id="cor1">
<label></label>
Corresponding author. Tel.: +65 65869625; fax: +65 67791117.
<email>mcbtanyj@imcb.a-star.edu.sg</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release">
<day>11</day>
<month>9</month>
<year>2006</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub">
<month>12</month>
<year>2006</year>
</pub-date>
<pub-date pub-type="epub">
<day>11</day>
<month>9</month>
<year>2006</year>
</pub-date>
<volume>122</volume>
<issue>1</issue>
<fpage>20</fpage>
<lpage>27</lpage>
<history>
<date date-type="received">
<day>21</day>
<month>3</month>
<year>2006</year>
</date>
<date date-type="rev-recd">
<day>24</day>
<month>5</month>
<year>2006</year>
</date>
<date date-type="accepted">
<day>7</day>
<month>6</month>
<year>2006</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright © 2006 Elsevier B.V. All rights reserved.</copyright-statement>
<copyright-year>2006</copyright-year>
<copyright-holder>Elsevier B.V.</copyright-holder>
<license>
<license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract>
<p>The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bΔ124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6 h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bΔ124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.</p>
</abstract>
<kwd-group>
<title>Keywords</title>
<kwd>Coronavirus</kwd>
<kwd>SARS coronavirus</kwd>
<kwd>Necrosis</kwd>
<kwd>Apoptosis</kwd>
<kwd>3b Protein</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations>
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<noCountry>
<name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name>
<name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C." last="Fielding">Burtram C. Fielding</name>
<name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name>
<name sortKey="Khan, Sehaam" sort="Khan, Sehaam" uniqKey="Khan S" first="Sehaam" last="Khan">Sehaam Khan</name>
<name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name>
<name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name>
<name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name>
<name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
</noCountry>
</tree>
</affiliations>
</record>

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