SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro
Identifieur interne : 000C75 ( Pmc/Checkpoint ); précédent : 000C74; suivant : 000C76SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro
Auteurs : Susan F. Dosch [États-Unis] ; Supriya D. Mahajan [États-Unis] ; Arlene R. Collins [États-Unis]Source :
- Virus Research [ 0168-1702 ] ; 2009.
Abstract
A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (
Url:
DOI: 10.1016/j.virusres.2009.01.005
PubMed: 19185596
PubMed Central: 2699111
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro</title>
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<author><name sortKey="Collins, Arlene R" sort="Collins, Arlene R" uniqKey="Collins A" first="Arlene R." last="Collins">Arlene R. Collins</name>
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<series><title level="j">Virus Research</title>
<idno type="ISSN">0168-1702</idno>
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<front><div type="abstract" xml:lang="en"><p>A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (<italic>p</italic>
= 0.003) compared to mock-treated controls. The increase in IL-8 stimulated by a concentration of 5.6 nM of S was comparable to concentrations seen for S protein binding to ACE2 or to neutralizing monoclonal antibody suggesting a physiological relevance. An NF-κB inhibitor, TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus-infected PBMC. Activation and translocation of NF-κB was shown to occur rapidly following exposure of PBMC or THP-1 cells to S protein using a highly sensitive assay for active nuclear NF-κB p65 transcription factor. The results further suggested that released or secreted S protein could activate blood monocytes through recognition by toll-like receptor (TLR)2 ligand.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Res</journal-id>
<journal-id journal-id-type="iso-abbrev">Virus Res</journal-id>
<journal-title-group><journal-title>Virus Research</journal-title>
</journal-title-group>
<issn pub-type="ppub">0168-1702</issn>
<issn pub-type="epub">1872-7492</issn>
<publisher><publisher-name>Elsevier B.V.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19185596</article-id>
<article-id pub-id-type="pmc">2699111</article-id>
<article-id pub-id-type="publisher-id">S0168-1702(09)00012-4</article-id>
<article-id pub-id-type="doi">10.1016/j.virusres.2009.01.005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Dosch</surname>
<given-names>Susan F.</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mahajan</surname>
<given-names>Supriya D.</given-names>
</name>
<xref rid="aff2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Collins</surname>
<given-names>Arlene R.</given-names>
</name>
<email>acollins@buffalo.edu</email>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</aff>
<aff id="aff2"><label>b</label>
Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author at: Department of Microbiology and Immunology, 138 Farber Hall, State University of New York at Buffalo, Buffalo NY 14214-3000, United States. Tel.: +1 716 829 2161; fax: +1 716 829 2158. <email>acollins@buffalo.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>29</day>
<month>1</month>
<year>2009</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>6</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>29</day>
<month>1</month>
<year>2009</year>
</pub-date>
<volume>142</volume>
<issue>1</issue>
<fpage>19</fpage>
<lpage>27</lpage>
<history><date date-type="received"><day>28</day>
<month>11</month>
<year>2008</year>
</date>
<date date-type="accepted"><day>9</day>
<month>1</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2009 Elsevier B.V. All rights reserved.</copyright-statement>
<copyright-year>2009</copyright-year>
<copyright-holder>Elsevier B.V.</copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract><p>A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (<italic>p</italic>
= 0.003) compared to mock-treated controls. The increase in IL-8 stimulated by a concentration of 5.6 nM of S was comparable to concentrations seen for S protein binding to ACE2 or to neutralizing monoclonal antibody suggesting a physiological relevance. An NF-κB inhibitor, TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus-infected PBMC. Activation and translocation of NF-κB was shown to occur rapidly following exposure of PBMC or THP-1 cells to S protein using a highly sensitive assay for active nuclear NF-κB p65 transcription factor. The results further suggested that released or secreted S protein could activate blood monocytes through recognition by toll-like receptor (TLR)2 ligand.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd>SARS</kwd>
<kwd>Spike protein</kwd>
<kwd>Human monocyte macrophages</kwd>
<kwd>NF-κB activation</kwd>
<kwd>Innate immunity</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>État de New York</li>
</region>
</list>
<tree><country name="États-Unis"><region name="État de New York"><name sortKey="Dosch, Susan F" sort="Dosch, Susan F" uniqKey="Dosch S" first="Susan F." last="Dosch">Susan F. Dosch</name>
</region>
<name sortKey="Collins, Arlene R" sort="Collins, Arlene R" uniqKey="Collins A" first="Arlene R." last="Collins">Arlene R. Collins</name>
<name sortKey="Mahajan, Supriya D" sort="Mahajan, Supriya D" uniqKey="Mahajan S" first="Supriya D." last="Mahajan">Supriya D. Mahajan</name>
</country>
</tree>
</affiliations>
</record>
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