SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro
Identifieur interne : 000C75 ( Pmc/Checkpoint ); précédent : 000C74; suivant : 000C76SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro
Auteurs : Susan F. Dosch [États-Unis] ; Supriya D. Mahajan [États-Unis] ; Arlene R. Collins [États-Unis]Source :
- Virus Research [ 0168-1702 ] ; 2009.
Abstract
A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (
Url:
DOI: 10.1016/j.virusres.2009.01.005
PubMed: 19185596
PubMed Central: 2699111
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:2699111Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro</title><author><name sortKey="Dosch, Susan F" sort="Dosch, Susan F" uniqKey="Dosch S" first="Susan F." last="Dosch">Susan F. Dosch</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Mahajan, Supriya D" sort="Mahajan, Supriya D" uniqKey="Mahajan S" first="Supriya D." last="Mahajan">Supriya D. Mahajan</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Collins, Arlene R" sort="Collins, Arlene R" uniqKey="Collins A" first="Arlene R." last="Collins">Arlene R. Collins</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19185596</idno><idno type="pmc">2699111</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699111</idno><idno type="RBID">PMC:2699111</idno><idno type="doi">10.1016/j.virusres.2009.01.005</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">001594</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001594</idno><idno type="wicri:Area/Pmc/Curation">001594</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001594</idno><idno type="wicri:Area/Pmc/Checkpoint">000C75</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000C75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro</title><author><name sortKey="Dosch, Susan F" sort="Dosch, Susan F" uniqKey="Dosch S" first="Susan F." last="Dosch">Susan F. Dosch</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Mahajan, Supriya D" sort="Mahajan, Supriya D" uniqKey="Mahajan S" first="Supriya D." last="Mahajan">Supriya D. Mahajan</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Collins, Arlene R" sort="Collins, Arlene R" uniqKey="Collins A" first="Arlene R." last="Collins">Arlene R. Collins</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j">Virus Research</title><idno type="ISSN">0168-1702</idno><idno type="eISSN">1872-7492</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (<italic>p</italic> = 0.003) compared to mock-treated controls. The increase in IL-8 stimulated by a concentration of 5.6 nM of S was comparable to concentrations seen for S protein binding to ACE2 or to neutralizing monoclonal antibody suggesting a physiological relevance. An NF-κB inhibitor, TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus-infected PBMC. Activation and translocation of NF-κB was shown to occur rapidly following exposure of PBMC or THP-1 cells to S protein using a highly sensitive assay for active nuclear NF-κB p65 transcription factor. The results further suggested that released or secreted S protein could activate blood monocytes through recognition by toll-like receptor (TLR)2 ligand.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Barton, G M" uniqKey="Barton G">G.M. Barton</name></author><author><name sortKey="Medzhitov, R" uniqKey="Medzhitov R">R. Medzhitov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beniac, D R" uniqKey="Beniac D">D.R. Beniac</name></author><author><name sortKey="Andonov, A" uniqKey="Andonov A">A. Andonov</name></author><author><name sortKey="Grudeski, E" uniqKey="Grudeski E">E. Grudeski</name></author><author><name sortKey="Booth, T F" uniqKey="Booth T">T.F. Booth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Bartelink, W" uniqKey="Bartelink W">W. Bartelink</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cameron, M J" uniqKey="Cameron M">M.J. Cameron</name></author><author><name sortKey="Ran, L" uniqKey="Ran L">L. Ran</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L. Xu</name></author><author><name sortKey="Danesh, A" uniqKey="Danesh A">A. Danesh</name></author><author><name sortKey="Bermejo Martin, J F" uniqKey="Bermejo Martin J">J.F. Bermejo-Martin</name></author><author><name sortKey="Cameron, C M" uniqKey="Cameron C">C.M. Cameron</name></author><author><name sortKey="Muller, M P" uniqKey="Muller M">M.P. Muller</name></author><author><name sortKey="Gold, W L" uniqKey="Gold W">W.L. Gold</name></author><author><name sortKey="Richardson, S E" uniqKey="Richardson S">S.E. Richardson</name></author><author><name sortKey="Poutanen, S M" uniqKey="Poutanen S">S.M. Poutanen</name></author><author><name sortKey="Willey, B M" uniqKey="Willey B">B.M. Willey</name></author><author><name sortKey="Devries, M E" uniqKey="Devries M">M.E. DeVries</name></author><author><name sortKey="Fang, Y" uniqKey="Fang Y">Y. Fang</name></author><author><name sortKey="Seneviratne, C" uniqKey="Seneviratne C">C. Seneviratne</name></author><author><name sortKey="Bosinger, S E" uniqKey="Bosinger S">S.E. Bosinger</name></author><author><name sortKey="Persad, D" uniqKey="Persad D">D. Persad</name></author><author><name sortKey="Wilkinson, P" uniqKey="Wilkinson P">P. Wilkinson</name></author><author><name sortKey="Greller, L D" uniqKey="Greller L">L.D. Greller</name></author><author><name sortKey="Somogyi, R" uniqKey="Somogyi R">R. Somogyi</name></author><author><name sortKey="Humar, A" uniqKey="Humar A">A. Humar</name></author><author><name sortKey="Keshavjee, S" uniqKey="Keshavjee S">S. Keshavjee</name></author><author><name sortKey="Louie, M" uniqKey="Louie M">M. Louie</name></author><author><name sortKey="Loeb, M B" uniqKey="Loeb M">M.B. Loeb</name></author><author><name sortKey="Brunton, J" uniqKey="Brunton J">J. Brunton</name></author><author><name sortKey="Mcgeer, A J" uniqKey="Mcgeer A">A.J. McGeer</name></author><author><name sortKey="Kelvin, D J" uniqKey="Kelvin D">D.J. Kelvin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, Y J" uniqKey="Chang Y">Y.J. Chang</name></author><author><name sortKey="Liu, C Y Y" uniqKey="Liu C">C.Y.-Y. Liu</name></author><author><name sortKey="Chiang, B L" uniqKey="Chiang B">B.L. Chiang</name></author><author><name sortKey="Chao, Y C" uniqKey="Chao Y">Y.C. Chao</name></author><author><name sortKey="Chen, C C" uniqKey="Chen C">C.C. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, J" uniqKey="Chen J">J. Chen</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K. Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheung, C Y" uniqKey="Cheung C">C.Y. Cheung</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author><author><name sortKey="Ng, I H" uniqKey="Ng I">I.H. Ng</name></author><author><name sortKey="Luk, W" uniqKey="Luk W">W. Luk</name></author><author><name sortKey="Sia, S F" uniqKey="Sia S">S.F. Sia</name></author><author><name sortKey="Wu, M H" uniqKey="Wu M">M.H. Wu</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Gordon, S" uniqKey="Gordon S">S. Gordon</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, A R" uniqKey="Collins A">A.R. Collins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, A R" uniqKey="Collins A">A.R. Collins</name></author><author><name sortKey="Grubb, A" uniqKey="Grubb A">A. Grubb</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corver, J" uniqKey="Corver J">J. Corver</name></author><author><name sortKey="Broer, R" uniqKey="Broer R">R. Broer</name></author><author><name sortKey="Van Kasteren, P" uniqKey="Van Kasteren P">P. van Kasteren</name></author><author><name sortKey="Spaan, W" uniqKey="Spaan W">W. Spaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davis, J N" uniqKey="Davis J">J.N. Davis</name></author><author><name sortKey="Kucuk, O" uniqKey="Kucuk O">O. Kucuk</name></author><author><name sortKey="Djuric, Z" uniqKey="Djuric Z">Z. Djuric</name></author><author><name sortKey="Sarkar, F H" uniqKey="Sarkar F">F.H. Sarkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L. Du</name></author><author><name sortKey="Kao, R Y" uniqKey="Kao R">R.Y. Kao</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y. He</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G. Zhao</name></author><author><name sortKey="Wong, C" uniqKey="Wong C">C. Wong</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K.Y. Yuen</name></author><author><name sortKey="Jin, D Y" uniqKey="Jin D">D.Y. Jin</name></author><author><name sortKey="Zheng, B J" uniqKey="Zheng B">B.J. Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elass, E" uniqKey="Elass E">E. Elass</name></author><author><name sortKey="Aubry, L" uniqKey="Aubry L">L. Aubry</name></author><author><name sortKey="Masson, M" uniqKey="Masson M">M. Masson</name></author><author><name sortKey="Denys, A" uniqKey="Denys A">A. Denys</name></author><author><name sortKey="Guerardel, Y" uniqKey="Guerardel Y">Y. Guérardel</name></author><author><name sortKey="Maes, E" uniqKey="Maes E">E. Maes</name></author><author><name sortKey="Legrand, D" uniqKey="Legrand D">D. Legrand</name></author><author><name sortKey="Mazurier, J" uniqKey="Mazurier J">J. Mazurier</name></author><author><name sortKey="Kremer, L" uniqKey="Kremer L">L. Kremer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, J" uniqKey="Fan J">J. Fan</name></author><author><name sortKey="Ye, R D" uniqKey="Ye R">R.D. Ye</name></author><author><name sortKey="Malik, A B" uniqKey="Malik A">A.B. Malik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Finberg, R W" uniqKey="Finberg R">R.W. Finberg</name></author><author><name sortKey="Wang, J P" uniqKey="Wang J">J.P. Wang</name></author><author><name sortKey="Kurt Jones, E A" uniqKey="Kurt Jones E">E.A. Kurt-Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frieman, M" uniqKey="Frieman M">M. Frieman</name></author><author><name sortKey="Heise, M" uniqKey="Heise M">M. Heise</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R. Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Y" uniqKey="He Y">Y. He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y. Zhou</name></author><author><name sortKey="Siddiqui, P" uniqKey="Siddiqui P">P. Siddiqui</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, L" uniqKey="He L">L. He</name></author><author><name sortKey="Ding, Y" uniqKey="Ding Y">Y. Ding</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q. Zhang</name></author><author><name sortKey="Che, X" uniqKey="Che X">X. Che</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y. He</name></author><author><name sortKey="Shen, H" uniqKey="Shen H">H. Shen</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z. Li</name></author><author><name sortKey="Zhao, L" uniqKey="Zhao L">L. Zhao</name></author><author><name sortKey="Geng, J" uniqKey="Geng J">J. Geng</name></author><author><name sortKey="Deng, Y" uniqKey="Deng Y">Y. Deng</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L. Yang</name></author><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Cai, J" uniqKey="Cai J">J. Cai</name></author><author><name sortKey="Qiu, L" uniqKey="Qiu L">L. Qiu</name></author><author><name sortKey="Wen, K" uniqKey="Wen K">K. Wen</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X. Xu</name></author><author><name sortKey="Jiang, S" uniqKey="Jiang S">S. Jiang</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Henkel, T" uniqKey="Henkel T">T. Henkel</name></author><author><name sortKey="Machleidt, T" uniqKey="Machleidt T">T. Machleidt</name></author><author><name sortKey="Alkalay, I" uniqKey="Alkalay I">I. Alkalay</name></author><author><name sortKey="Kronke, M" uniqKey="Kronke M">M. Krönke</name></author><author><name sortKey="Ben Neriah, Y" uniqKey="Ben Neriah Y">Y. Ben-Neriah</name></author><author><name sortKey="Baeuerle, P A" uniqKey="Baeuerle P">P.A. Baeuerle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hillian, A D" uniqKey="Hillian A">A.D. Hillian</name></author><author><name sortKey="Londono, D" uniqKey="Londono D">D. Londono</name></author><author><name sortKey="Dunn, J M" uniqKey="Dunn J">J.M. Dunn</name></author><author><name sortKey="Goddard, K A" uniqKey="Goddard K">K.A. Goddard</name></author><author><name sortKey="Pace, R G" uniqKey="Pace R">R.G. Pace</name></author><author><name sortKey="Knowles, M R" uniqKey="Knowles M">M.R. Knowles</name></author><author><name sortKey="Drumm, M L" uniqKey="Drumm M">M.L. Drumm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hsueh, P R" uniqKey="Hsueh P">P.R. Hsueh</name></author><author><name sortKey="Chen, P J" uniqKey="Chen P">P.J. Chen</name></author><author><name sortKey="Hsiao, C H" uniqKey="Hsiao C">C.H. Hsiao</name></author><author><name sortKey="Yeh, S H" uniqKey="Yeh S">S.H. Yeh</name></author><author><name sortKey="Cheng, W C" uniqKey="Cheng W">W.C. Cheng</name></author><author><name sortKey="Wang, J L" uniqKey="Wang J">J.L. Wang</name></author><author><name sortKey="Chiang, B L" uniqKey="Chiang B">B.L. Chiang</name></author><author><name sortKey="Chang, S C" uniqKey="Chang S">S.C. Chang</name></author><author><name sortKey="Chang, F Y" uniqKey="Chang F">F.Y. Chang</name></author><author><name sortKey="Wong, W W" uniqKey="Wong W">W.W. Wong</name></author><author><name sortKey="Kao, C L" uniqKey="Kao C">C.L. Kao</name></author><author><name sortKey="Yang, P C" uniqKey="Yang P">P.C. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, I C" uniqKey="Huang I">I.C. Huang</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.J. Bosch</name></author><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Lee, K H" uniqKey="Lee K">K.H. Lee</name></author><author><name sortKey="Ghiran, S" uniqKey="Ghiran S">S. Ghiran</name></author><author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N. Vasilieva</name></author><author><name sortKey="Dermody, T S" uniqKey="Dermody T">T.S. Dermody</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author><author><name sortKey="Dormitzer, P R" uniqKey="Dormitzer P">P.R. Dormitzer</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Rottier, P J" uniqKey="Rottier P">P.J. Rottier</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Inoue, Y" uniqKey="Inoue Y">Y. Inoue</name></author><author><name sortKey="Tanaka, N" uniqKey="Tanaka N">N. Tanaka</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y. Tanaka</name></author><author><name sortKey="Inoue, S" uniqKey="Inoue S">S. Inoue</name></author><author><name sortKey="Morita, K" uniqKey="Morita K">K. Morita</name></author><author><name sortKey="Zhuang, M" uniqKey="Zhuang M">M. Zhuang</name></author><author><name sortKey="Hattori, T" uniqKey="Hattori T">T. Hattori</name></author><author><name sortKey="Sugamura, K" uniqKey="Sugamura K">K. Sugamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jovasevic, V" uniqKey="Jovasevic V">V. Jovasevic</name></author><author><name sortKey="Liang, L" uniqKey="Liang L">L. Liang</name></author><author><name sortKey="Roizman, B" uniqKey="Roizman B">B. Roizman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawase, M" uniqKey="Kawase M">M. Kawase</name></author><author><name sortKey="Shirato, K" uniqKey="Shirato K">K. Shirato</name></author><author><name sortKey="Matsuyama, S" uniqKey="Matsuyama S">S. Matsuyama</name></author><author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F. Taguchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, F" uniqKey="Li F">F. Li</name></author><author><name sortKey="Berardi, M" uniqKey="Berardi M">M. Berardi</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Dormitzer, P R" uniqKey="Dormitzer P">P.R. Dormitzer</name></author><author><name sortKey="Harrison, S C" uniqKey="Harrison S">S.C. Harrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, L" uniqKey="Li L">L. Li</name></author><author><name sortKey="Wo, J" uniqKey="Wo J">J. Wo</name></author><author><name sortKey="Shao, J" uniqKey="Shao J">J. Shao</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H. Zhu</name></author><author><name sortKey="Wu, N" uniqKey="Wu N">N. Wu</name></author><author><name sortKey="Li, M" uniqKey="Li M">M. Li</name></author><author><name sortKey="Yao, H" uniqKey="Yao H">H. Yao</name></author><author><name sortKey="Hu, M" uniqKey="Hu M">M. Hu</name></author><author><name sortKey="Dennin, R H" uniqKey="Dennin R">R.H. Dennin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name></author><author><name sortKey="Vasilieva, N" uniqKey="Vasilieva N">N. Vasilieva</name></author><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name></author><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K. Wong</name></author><author><name sortKey="Berne, M A" uniqKey="Berne M">M.A. Berne</name></author><author><name sortKey="Somasundaran, M" uniqKey="Somasundaran M">M. Somasundaran</name></author><author><name sortKey="Sullivan, J L" uniqKey="Sullivan J">J.L. Sullivan</name></author><author><name sortKey="Luzuriaga, K" uniqKey="Luzuriaga K">K. Luzuriaga</name></author><author><name sortKey="Greenough, T C" uniqKey="Greenough T">T.C. Greenough</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q. Li</name></author><author><name sortKey="Verma, I M" uniqKey="Verma I">I.M. Verma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, Z" uniqKey="Luo Z">Z. Luo</name></author><author><name sortKey="Matthews, A M" uniqKey="Matthews A">A.M. Matthews</name></author><author><name sortKey="Weiss, S R" uniqKey="Weiss S">S.R. Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maeda, J" uniqKey="Maeda J">J. Maeda</name></author><author><name sortKey="Repass, J F" uniqKey="Repass J">J.F. Repass</name></author><author><name sortKey="Maeda, A" uniqKey="Maeda A">A. Maeda</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mahajan, S D" uniqKey="Mahajan S">S.D. Mahajan</name></author><author><name sortKey="Schwartz, S A" uniqKey="Schwartz S">S.A. Schwartz</name></author><author><name sortKey="Aalinkeel, R" uniqKey="Aalinkeel R">R. Aalinkeel</name></author><author><name sortKey="Chawda, R P" uniqKey="Chawda R">R.P. Chawda</name></author><author><name sortKey="Sykes, D E" uniqKey="Sykes D">D.E. Sykes</name></author><author><name sortKey="Nair, M P" uniqKey="Nair M">M.P. Nair</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mizzen, L" uniqKey="Mizzen L">L. Mizzen</name></author><author><name sortKey="Daya, M" uniqKey="Daya M">M. Daya</name></author><author><name sortKey="Anderson, R" uniqKey="Anderson R">R. Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pulford, D J" uniqKey="Pulford D">D.J. Pulford</name></author><author><name sortKey="Britton, P" uniqKey="Britton P">P. Britton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reghunathan, R" uniqKey="Reghunathan R">R. Reghunathan</name></author><author><name sortKey="Jayapal, M" uniqKey="Jayapal M">M. Jayapal</name></author><author><name sortKey="Hsu, L Y" uniqKey="Hsu L">L.Y. Hsu</name></author><author><name sortKey="Chng, H H" uniqKey="Chng H">H.H. Chng</name></author><author><name sortKey="Tai, D" uniqKey="Tai D">D. Tai</name></author><author><name sortKey="Leung, B P" uniqKey="Leung B">B.P. Leung</name></author><author><name sortKey="Melendez, A J" uniqKey="Melendez A">A.J. Melendez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rosenau, C" uniqKey="Rosenau C">C. Rosenau</name></author><author><name sortKey="Emery, D" uniqKey="Emery D">D. Emery</name></author><author><name sortKey="Kaboord, B" uniqKey="Kaboord B">B. Kaboord</name></author><author><name sortKey="Qaranfleh, M W" uniqKey="Qaranfleh M">M.W. Qaranfleh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shao, H" uniqKey="Shao H">H. Shao</name></author><author><name sortKey="Lan, D" uniqKey="Lan D">D. Lan</name></author><author><name sortKey="Duan, Z" uniqKey="Duan Z">Z. Duan</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name></author><author><name sortKey="Min, J" uniqKey="Min J">J. Min</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author><author><name sortKey="Huang, J" uniqKey="Huang J">J. Huang</name></author><author><name sortKey="Su, J" uniqKey="Su J">J. Su</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S. Chen</name></author><author><name sortKey="Xu, A" uniqKey="Xu A">A. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, H C" uniqKey="Song H">H.C. Song</name></author><author><name sortKey="Seo, M Y" uniqKey="Seo M">M.Y. Seo</name></author><author><name sortKey="Stadler, K" uniqKey="Stadler K">K. Stadler</name></author><author><name sortKey="Yoo, B J" uniqKey="Yoo B">B.J. Yoo</name></author><author><name sortKey="Choo, Q L" uniqKey="Choo Q">Q.L. Choo</name></author><author><name sortKey="Coates, S R" uniqKey="Coates S">S.R. Coates</name></author><author><name sortKey="Uematsu, Y" uniqKey="Uematsu Y">Y. Uematsu</name></author><author><name sortKey="Harada, T" uniqKey="Harada T">T. Harada</name></author><author><name sortKey="Greer, C E" uniqKey="Greer C">C.E. Greer</name></author><author><name sortKey="Polo, J M" uniqKey="Polo J">J.M. Polo</name></author><author><name sortKey="Pileri, P" uniqKey="Pileri P">P. Pileri</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M. Eickmann</name></author><author><name sortKey="Rappuoli, R" uniqKey="Rappuoli R">R. Rappuoli</name></author><author><name sortKey="Abrignani, S" uniqKey="Abrignani S">S. Abrignani</name></author><author><name sortKey="Houghton, M" uniqKey="Houghton M">M. Houghton</name></author><author><name sortKey="Han, J H" uniqKey="Han J">J.H. Han</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, J" uniqKey="Sui J">J. Sui</name></author><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name></author><author><name sortKey="Murakami, A" uniqKey="Murakami A">A. Murakami</name></author><author><name sortKey="Tamin, A" uniqKey="Tamin A">A. Tamin</name></author><author><name sortKey="Matthews, L J" uniqKey="Matthews L">L.J. Matthews</name></author><author><name sortKey="Wong, S K" uniqKey="Wong S">S.K. Wong</name></author><author><name sortKey="Moore, M J" uniqKey="Moore M">M.J. Moore</name></author><author><name sortKey="Tallarico, A S" uniqKey="Tallarico A">A.S. Tallarico</name></author><author><name sortKey="Olurinde, M" uniqKey="Olurinde M">M. Olurinde</name></author><author><name sortKey="Choe, H" uniqKey="Choe H">H. Choe</name></author><author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name></author><author><name sortKey="Bellini, W J" uniqKey="Bellini W">W.J. Bellini</name></author><author><name sortKey="Farzan, M" uniqKey="Farzan M">M. Farzan</name></author><author><name sortKey="Marasco, W A" uniqKey="Marasco W">W.A. Marasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thorp, E B" uniqKey="Thorp E">E.B. Thorp</name></author><author><name sortKey="Boscarino, J A" uniqKey="Boscarino J">J.A. Boscarino</name></author><author><name sortKey="Logan, H L" uniqKey="Logan H">H.L. Logan</name></author><author><name sortKey="Goletz, J T" uniqKey="Goletz J">J.T. Goletz</name></author><author><name sortKey="Gallagher, T M" uniqKey="Gallagher T">T.M. Gallagher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tseng, C T" uniqKey="Tseng C">C.T. Tseng</name></author><author><name sortKey="Perrone, L A" uniqKey="Perrone L">L.A. Perrone</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H. Zhu</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S. Makino</name></author><author><name sortKey="Peters, C J" uniqKey="Peters C">C.J. Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uematsu, S" uniqKey="Uematsu S">S. Uematsu</name></author><author><name sortKey="Akria, S" uniqKey="Akria S">S. Akria</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Versteeg, G A" uniqKey="Versteeg G">G.A. Versteeg</name></author><author><name sortKey="Van De Nes, P S" uniqKey="Van De Nes P">P.S. van de Nes</name></author><author><name sortKey="Breedenbeek, P J" uniqKey="Breedenbeek P">P.J. Breedenbeek</name></author><author><name sortKey="Spaan, W J M" uniqKey="Spaan W">W.J.M. Spaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Walsh, K B" uniqKey="Walsh K">K.B. Walsh</name></author><author><name sortKey="Lodoen, M B" uniqKey="Lodoen M">M.B. Lodoen</name></author><author><name sortKey="Edwards, R A" uniqKey="Edwards R">R.A. Edwards</name></author><author><name sortKey="Lanier, L L" uniqKey="Lanier L">L.L. Lanier</name></author><author><name sortKey="Lane, T E" uniqKey="Lane T">T.E. Lane</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Ye, Linbai" uniqKey="Ye L">Linbai Ye</name></author><author><name sortKey="Ye, L" uniqKey="Ye L">L. Ye</name></author><author><name sortKey="Li, B" uniqKey="Li B">B. Li</name></author><author><name sortKey="Gao, B" uniqKey="Gao B">B. Gao</name></author><author><name sortKey="Zeng, Y" uniqKey="Zeng Y">Y. Zeng</name></author><author><name sortKey="Kong, L" uniqKey="Kong L">L. Kong</name></author><author><name sortKey="Fang, X" uniqKey="Fang X">X. Fang</name></author><author><name sortKey="Zheng, H" uniqKey="Zheng H">H. Zheng</name></author><author><name sortKey="Wu, Z" uniqKey="Wu Z">Z. Wu</name></author><author><name sortKey="She, Y" uniqKey="She Y">Y. She</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolk, D M" uniqKey="Wolk D">D.M. Wolk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeager, C L" uniqKey="Yeager C">C.L. Yeager</name></author><author><name sortKey="Ashmun, R A" uniqKey="Ashmun R">R.A. Ashmun</name></author><author><name sortKey="Williams, R K" uniqKey="Williams R">R.K. Williams</name></author><author><name sortKey="Cardellichio, C B" uniqKey="Cardellichio C">C.B. Cardellichio</name></author><author><name sortKey="Shapiro, L H" uniqKey="Shapiro L">L.H. Shapiro</name></author><author><name sortKey="Look, A T" uniqKey="Look A">A.T. Look</name></author><author><name sortKey="Holmes, K V" uniqKey="Holmes K">K.V. Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, S Y" uniqKey="Yu S">S.-Y. Yu</name></author><author><name sortKey="Hu, Y W" uniqKey="Hu Y">Y.-W. Hu</name></author><author><name sortKey="Liu, X Y" uniqKey="Liu X">X.-Y. Liu</name></author><author><name sortKey="Xiong, W" uniqKey="Xiong W">W. Xiong</name></author><author><name sortKey="Zhou, Z T" uniqKey="Zhou Z">Z.-T. Zhou</name></author><author><name sortKey="Yuan, Z H" uniqKey="Yuan Z">Z.-H. Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, H" uniqKey="Zhu H">H. Zhu</name></author><author><name sortKey="Dinsdale, D" uniqKey="Dinsdale D">D. Dinsdale</name></author><author><name sortKey="Alnemri, E S" uniqKey="Alnemri E">E.S. Alnemri</name></author><author><name sortKey="Cohen, G M" uniqKey="Cohen G">G.M. Cohen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Res</journal-id><journal-id journal-id-type="iso-abbrev">Virus Res</journal-id><journal-title-group><journal-title>Virus Research</journal-title></journal-title-group><issn pub-type="ppub">0168-1702</issn><issn pub-type="epub">1872-7492</issn><publisher><publisher-name>Elsevier B.V.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19185596</article-id><article-id pub-id-type="pmc">2699111</article-id><article-id pub-id-type="publisher-id">S0168-1702(09)00012-4</article-id><article-id pub-id-type="doi">10.1016/j.virusres.2009.01.005</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dosch</surname><given-names>Susan F.</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Mahajan</surname><given-names>Supriya D.</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Collins</surname><given-names>Arlene R.</given-names></name><email>acollins@buffalo.edu</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Department of Microbiology and Immunology, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</aff><aff id="aff2"><label>b</label>Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo, NY, United States</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author at: Department of Microbiology and Immunology, 138 Farber Hall, State University of New York at Buffalo, Buffalo NY 14214-3000, United States. Tel.: +1 716 829 2161; fax: +1 716 829 2158. <email>acollins@buffalo.edu</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>29</day><month>1</month><year>2009</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>6</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>29</day><month>1</month><year>2009</year></pub-date><volume>142</volume><issue>1</issue><fpage>19</fpage><lpage>27</lpage><history><date date-type="received"><day>28</day><month>11</month><year>2008</year></date><date date-type="accepted"><day>9</day><month>1</month><year>2009</year></date></history><permissions><copyright-statement>Copyright © 2009 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2009</copyright-year><copyright-holder>Elsevier B.V.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>A purified recombinant spike (S) protein was studied for its effect on stimulating human peripheral blood monocyte macrophages (PBMC). We examined inflammatory gene mRNA abundances found in S protein-treated PBMC using gene arrays. We identified differential mRNA abundances of genes with functional properties associated with antiviral (CXCL10) and inflammatory (IL-6 and IL-8) responses. We confirmed cytokine mRNA increases by real-time quantitative(q) RT-PCR or ELISA. We further analyzed the sensitivity and specificity of the prominent IL-8 response. By real-time qRT-PCR, S protein was shown to stimulate IL-8 mRNA accumulation in a dose dependent manner while treatment with E protein did not. Also, titration of S protein-specific production and secretion of IL-8 by ELISA showed that the dose of 5.6 nM of S produced a significant increase in IL-8 (<italic>p</italic> = 0.003) compared to mock-treated controls. The increase in IL-8 stimulated by a concentration of 5.6 nM of S was comparable to concentrations seen for S protein binding to ACE2 or to neutralizing monoclonal antibody suggesting a physiological relevance. An NF-κB inhibitor, TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus-infected PBMC. Activation and translocation of NF-κB was shown to occur rapidly following exposure of PBMC or THP-1 cells to S protein using a highly sensitive assay for active nuclear NF-κB p65 transcription factor. The results further suggested that released or secreted S protein could activate blood monocytes through recognition by toll-like receptor (TLR)2 ligand.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>Spike protein</kwd><kwd>Human monocyte macrophages</kwd><kwd>NF-κB activation</kwd><kwd>Innate immunity</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Dosch, Susan F" sort="Dosch, Susan F" uniqKey="Dosch S" first="Susan F." last="Dosch">Susan F. Dosch</name></region><name sortKey="Collins, Arlene R" sort="Collins, Arlene R" uniqKey="Collins A" first="Arlene R." last="Collins">Arlene R. Collins</name><name sortKey="Mahajan, Supriya D" sort="Mahajan, Supriya D" uniqKey="Mahajan S" first="Supriya D." last="Mahajan">Supriya D. Mahajan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000C75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:2699111
|texte= SARS coronavirus spike protein-induced innate immune response occurs via activation of the NF-κB pathway in human monocyte macrophages in vitro
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:19185596" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |